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Insertion of the voltage-sensitive domain into circularly permuted red fluorescent protein as a design for genetically encoded voltage sensor

Visualization of electrical activity in living cells represents an important challenge in context of basic neurophysiological studies. Here we report a new voltage sensitive fluorescent indicator which response could be detected by fluorescence monitoring in a single red channel. To the best of our...

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Detalles Bibliográficos
Autores principales: Kost, Liubov A., Nikitin, Evgeny S., Ivanova, Violetta O., Sung, Uhna, Putintseva, Ekaterina V., Chudakov, Dmitry M., Balaban, Pavel M., Lukyanov, Konstantin A., Bogdanov, Alexey M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5580962/
https://www.ncbi.nlm.nih.gov/pubmed/28863184
http://dx.doi.org/10.1371/journal.pone.0184225