Cargando…
Altered erythropoiesis and decreased number of erythrocytes in children with neuroblastoma
Neuroblastoma (NB) is a pediatric tumor presenting at diagnosis either as localized or metastatic disease, which mainly involves the bone marrow (BM). The physical occupancy of BM space by metastatic NB cells has been held responsible for impairment of BM function. Here, we investigated whether loca...
Autores principales: | , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5581103/ https://www.ncbi.nlm.nih.gov/pubmed/28881804 http://dx.doi.org/10.18632/oncotarget.18285 |
_version_ | 1783260999628881920 |
---|---|
author | Morandi, Fabio Barco, Sebastiano Stigliani, Sara Croce, Michela Persico, Luca Lagazio, Corrado Scuderi, Francesca Belli, Maria Luisa Montera, Mariapina Cangemi, Giuliana Pozzi, Sarah Rigo, Valentina Scaruffi, Paola Amoroso, Loredana Erminio, Giovanni Pistoia, Vito Ferrini, Silvano Corrias, Maria Valeria |
author_facet | Morandi, Fabio Barco, Sebastiano Stigliani, Sara Croce, Michela Persico, Luca Lagazio, Corrado Scuderi, Francesca Belli, Maria Luisa Montera, Mariapina Cangemi, Giuliana Pozzi, Sarah Rigo, Valentina Scaruffi, Paola Amoroso, Loredana Erminio, Giovanni Pistoia, Vito Ferrini, Silvano Corrias, Maria Valeria |
author_sort | Morandi, Fabio |
collection | PubMed |
description | Neuroblastoma (NB) is a pediatric tumor presenting at diagnosis either as localized or metastatic disease, which mainly involves the bone marrow (BM). The physical occupancy of BM space by metastatic NB cells has been held responsible for impairment of BM function. Here, we investigated whether localized or metastatic NB may alter hematopoietic lineages’ maturation and release of mature cells in the periphery, through gene expression profiling, analysis of BM smears, cell blood count and flow cytometry analysis. Gene ontology and disease-associated analysis of the genes significantly under-expressed in BM resident cells from children with localized and metastatic NB, as compared to healthy children, indicated anemia, blood group antigens, and heme and porphyrin biosynthesis as major functional annotation clusters. Accordingly, in children with NB there was a selective impairment of erythrocyte maturation at the ortho-chromic stage that resulted in reduced erythrocyte count in the periphery, regardless of the presence of metastatic cells in the BM. By considering all NB patients, low erythrocyte count at diagnosis associated with worse survival. Moreover, in the subset of metastatic patients, low erythrocyte count, hemoglobin and hematocrit and high red cell distribution width at follow-up also associated with worse outcome. These observations provide an alternative model to the tenet that infiltrating cells inhibit BM functions due to physical occupancy of space and may open a new area of research in NB to understand the mechanism(s) responsible for such selective impairment. |
format | Online Article Text |
id | pubmed-5581103 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-55811032017-09-06 Altered erythropoiesis and decreased number of erythrocytes in children with neuroblastoma Morandi, Fabio Barco, Sebastiano Stigliani, Sara Croce, Michela Persico, Luca Lagazio, Corrado Scuderi, Francesca Belli, Maria Luisa Montera, Mariapina Cangemi, Giuliana Pozzi, Sarah Rigo, Valentina Scaruffi, Paola Amoroso, Loredana Erminio, Giovanni Pistoia, Vito Ferrini, Silvano Corrias, Maria Valeria Oncotarget Research Paper Neuroblastoma (NB) is a pediatric tumor presenting at diagnosis either as localized or metastatic disease, which mainly involves the bone marrow (BM). The physical occupancy of BM space by metastatic NB cells has been held responsible for impairment of BM function. Here, we investigated whether localized or metastatic NB may alter hematopoietic lineages’ maturation and release of mature cells in the periphery, through gene expression profiling, analysis of BM smears, cell blood count and flow cytometry analysis. Gene ontology and disease-associated analysis of the genes significantly under-expressed in BM resident cells from children with localized and metastatic NB, as compared to healthy children, indicated anemia, blood group antigens, and heme and porphyrin biosynthesis as major functional annotation clusters. Accordingly, in children with NB there was a selective impairment of erythrocyte maturation at the ortho-chromic stage that resulted in reduced erythrocyte count in the periphery, regardless of the presence of metastatic cells in the BM. By considering all NB patients, low erythrocyte count at diagnosis associated with worse survival. Moreover, in the subset of metastatic patients, low erythrocyte count, hemoglobin and hematocrit and high red cell distribution width at follow-up also associated with worse outcome. These observations provide an alternative model to the tenet that infiltrating cells inhibit BM functions due to physical occupancy of space and may open a new area of research in NB to understand the mechanism(s) responsible for such selective impairment. Impact Journals LLC 2017-05-30 /pmc/articles/PMC5581103/ /pubmed/28881804 http://dx.doi.org/10.18632/oncotarget.18285 Text en Copyright: © 2017 Morandi et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Research Paper Morandi, Fabio Barco, Sebastiano Stigliani, Sara Croce, Michela Persico, Luca Lagazio, Corrado Scuderi, Francesca Belli, Maria Luisa Montera, Mariapina Cangemi, Giuliana Pozzi, Sarah Rigo, Valentina Scaruffi, Paola Amoroso, Loredana Erminio, Giovanni Pistoia, Vito Ferrini, Silvano Corrias, Maria Valeria Altered erythropoiesis and decreased number of erythrocytes in children with neuroblastoma |
title | Altered erythropoiesis and decreased number of erythrocytes in children with neuroblastoma |
title_full | Altered erythropoiesis and decreased number of erythrocytes in children with neuroblastoma |
title_fullStr | Altered erythropoiesis and decreased number of erythrocytes in children with neuroblastoma |
title_full_unstemmed | Altered erythropoiesis and decreased number of erythrocytes in children with neuroblastoma |
title_short | Altered erythropoiesis and decreased number of erythrocytes in children with neuroblastoma |
title_sort | altered erythropoiesis and decreased number of erythrocytes in children with neuroblastoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5581103/ https://www.ncbi.nlm.nih.gov/pubmed/28881804 http://dx.doi.org/10.18632/oncotarget.18285 |
work_keys_str_mv | AT morandifabio alterederythropoiesisanddecreasednumberoferythrocytesinchildrenwithneuroblastoma AT barcosebastiano alterederythropoiesisanddecreasednumberoferythrocytesinchildrenwithneuroblastoma AT stiglianisara alterederythropoiesisanddecreasednumberoferythrocytesinchildrenwithneuroblastoma AT crocemichela alterederythropoiesisanddecreasednumberoferythrocytesinchildrenwithneuroblastoma AT persicoluca alterederythropoiesisanddecreasednumberoferythrocytesinchildrenwithneuroblastoma AT lagaziocorrado alterederythropoiesisanddecreasednumberoferythrocytesinchildrenwithneuroblastoma AT scuderifrancesca alterederythropoiesisanddecreasednumberoferythrocytesinchildrenwithneuroblastoma AT bellimarialuisa alterederythropoiesisanddecreasednumberoferythrocytesinchildrenwithneuroblastoma AT monteramariapina alterederythropoiesisanddecreasednumberoferythrocytesinchildrenwithneuroblastoma AT cangemigiuliana alterederythropoiesisanddecreasednumberoferythrocytesinchildrenwithneuroblastoma AT pozzisarah alterederythropoiesisanddecreasednumberoferythrocytesinchildrenwithneuroblastoma AT rigovalentina alterederythropoiesisanddecreasednumberoferythrocytesinchildrenwithneuroblastoma AT scaruffipaola alterederythropoiesisanddecreasednumberoferythrocytesinchildrenwithneuroblastoma AT amorosoloredana alterederythropoiesisanddecreasednumberoferythrocytesinchildrenwithneuroblastoma AT erminiogiovanni alterederythropoiesisanddecreasednumberoferythrocytesinchildrenwithneuroblastoma AT pistoiavito alterederythropoiesisanddecreasednumberoferythrocytesinchildrenwithneuroblastoma AT ferrinisilvano alterederythropoiesisanddecreasednumberoferythrocytesinchildrenwithneuroblastoma AT corriasmariavaleria alterederythropoiesisanddecreasednumberoferythrocytesinchildrenwithneuroblastoma |