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Aptamer-Targeted Plasmonic Photothermal Therapy of Cancer

Novel nanoscale bioconjugates combining unique plasmonic photothermal properties of gold nanoparticles (AuNPs) with targeted delivery using cell-specific DNA aptamers have a tremendous potential for medical diagnostics and therapy of many cell-based diseases. In this study, we demonstrate the high a...

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Autores principales: Kolovskaya, Olga S., Zamay, Tatiana N., Belyanina, Irina V., Karlova, Elena, Garanzha, Irina, Aleksandrovsky, Aleksandr S., Kirichenko, Andrey, Dubynina, Anna V., Sokolov, Alexey E., Zamay, Galina S., Glazyrin, Yury E., Zamay, Sergey, Ivanchenko, Tatiana, Chanchikova, Natalia, Tokarev, Nikolay, Shepelevich, Nikolay, Ozerskaya, Anastasia, Badrin, Evgeniy, Belugin, Kirill, Belkin, Simon, Zabluda, Vladimir, Gargaun, Ana, Berezovski, Maxim V., Kichkailo, Anna S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5582647/
https://www.ncbi.nlm.nih.gov/pubmed/29246290
http://dx.doi.org/10.1016/j.omtn.2017.08.007
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author Kolovskaya, Olga S.
Zamay, Tatiana N.
Belyanina, Irina V.
Karlova, Elena
Garanzha, Irina
Aleksandrovsky, Aleksandr S.
Kirichenko, Andrey
Dubynina, Anna V.
Sokolov, Alexey E.
Zamay, Galina S.
Glazyrin, Yury E.
Zamay, Sergey
Ivanchenko, Tatiana
Chanchikova, Natalia
Tokarev, Nikolay
Shepelevich, Nikolay
Ozerskaya, Anastasia
Badrin, Evgeniy
Belugin, Kirill
Belkin, Simon
Zabluda, Vladimir
Gargaun, Ana
Berezovski, Maxim V.
Kichkailo, Anna S.
author_facet Kolovskaya, Olga S.
Zamay, Tatiana N.
Belyanina, Irina V.
Karlova, Elena
Garanzha, Irina
Aleksandrovsky, Aleksandr S.
Kirichenko, Andrey
Dubynina, Anna V.
Sokolov, Alexey E.
Zamay, Galina S.
Glazyrin, Yury E.
Zamay, Sergey
Ivanchenko, Tatiana
Chanchikova, Natalia
Tokarev, Nikolay
Shepelevich, Nikolay
Ozerskaya, Anastasia
Badrin, Evgeniy
Belugin, Kirill
Belkin, Simon
Zabluda, Vladimir
Gargaun, Ana
Berezovski, Maxim V.
Kichkailo, Anna S.
author_sort Kolovskaya, Olga S.
collection PubMed
description Novel nanoscale bioconjugates combining unique plasmonic photothermal properties of gold nanoparticles (AuNPs) with targeted delivery using cell-specific DNA aptamers have a tremendous potential for medical diagnostics and therapy of many cell-based diseases. In this study, we demonstrate the high anti-cancer activity of aptamer-conjugated, 37-nm spherical gold nanoparticles toward Ehrlich carcinoma in tumor-bearing mice after photothermal treatment. The synthetic anti-tumor aptamers bring the nanoparticles precisely to the desired cells and selectively eliminate cancer cells after the subsequent laser treatment. To prove tumor eradication, we used positron emission tomography (PET) utilizing radioactive glucose and computer tomography, followed by histological analysis of cancer tissue. Three injections of aptamer-conjugated AuNPs and 5 min of laser irradiations are enough to make the tumor undetectable by PET. Histological analysis proves PET results and shows lower damage of healthy tissue in addition to a higher treatment efficiency and selectivity of the gold nanoparticles functionalized with aptamers in comparison to control experiments using free unconjugated nanoparticles.
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spelling pubmed-55826472017-10-03 Aptamer-Targeted Plasmonic Photothermal Therapy of Cancer Kolovskaya, Olga S. Zamay, Tatiana N. Belyanina, Irina V. Karlova, Elena Garanzha, Irina Aleksandrovsky, Aleksandr S. Kirichenko, Andrey Dubynina, Anna V. Sokolov, Alexey E. Zamay, Galina S. Glazyrin, Yury E. Zamay, Sergey Ivanchenko, Tatiana Chanchikova, Natalia Tokarev, Nikolay Shepelevich, Nikolay Ozerskaya, Anastasia Badrin, Evgeniy Belugin, Kirill Belkin, Simon Zabluda, Vladimir Gargaun, Ana Berezovski, Maxim V. Kichkailo, Anna S. Mol Ther Nucleic Acids Original Article Novel nanoscale bioconjugates combining unique plasmonic photothermal properties of gold nanoparticles (AuNPs) with targeted delivery using cell-specific DNA aptamers have a tremendous potential for medical diagnostics and therapy of many cell-based diseases. In this study, we demonstrate the high anti-cancer activity of aptamer-conjugated, 37-nm spherical gold nanoparticles toward Ehrlich carcinoma in tumor-bearing mice after photothermal treatment. The synthetic anti-tumor aptamers bring the nanoparticles precisely to the desired cells and selectively eliminate cancer cells after the subsequent laser treatment. To prove tumor eradication, we used positron emission tomography (PET) utilizing radioactive glucose and computer tomography, followed by histological analysis of cancer tissue. Three injections of aptamer-conjugated AuNPs and 5 min of laser irradiations are enough to make the tumor undetectable by PET. Histological analysis proves PET results and shows lower damage of healthy tissue in addition to a higher treatment efficiency and selectivity of the gold nanoparticles functionalized with aptamers in comparison to control experiments using free unconjugated nanoparticles. American Society of Gene & Cell Therapy 2017-08-16 /pmc/articles/PMC5582647/ /pubmed/29246290 http://dx.doi.org/10.1016/j.omtn.2017.08.007 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Article
Kolovskaya, Olga S.
Zamay, Tatiana N.
Belyanina, Irina V.
Karlova, Elena
Garanzha, Irina
Aleksandrovsky, Aleksandr S.
Kirichenko, Andrey
Dubynina, Anna V.
Sokolov, Alexey E.
Zamay, Galina S.
Glazyrin, Yury E.
Zamay, Sergey
Ivanchenko, Tatiana
Chanchikova, Natalia
Tokarev, Nikolay
Shepelevich, Nikolay
Ozerskaya, Anastasia
Badrin, Evgeniy
Belugin, Kirill
Belkin, Simon
Zabluda, Vladimir
Gargaun, Ana
Berezovski, Maxim V.
Kichkailo, Anna S.
Aptamer-Targeted Plasmonic Photothermal Therapy of Cancer
title Aptamer-Targeted Plasmonic Photothermal Therapy of Cancer
title_full Aptamer-Targeted Plasmonic Photothermal Therapy of Cancer
title_fullStr Aptamer-Targeted Plasmonic Photothermal Therapy of Cancer
title_full_unstemmed Aptamer-Targeted Plasmonic Photothermal Therapy of Cancer
title_short Aptamer-Targeted Plasmonic Photothermal Therapy of Cancer
title_sort aptamer-targeted plasmonic photothermal therapy of cancer
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5582647/
https://www.ncbi.nlm.nih.gov/pubmed/29246290
http://dx.doi.org/10.1016/j.omtn.2017.08.007
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