A tunable refractive index matching medium for live imaging cells, tissues and model organisms

In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic mediu...

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Detalles Bibliográficos
Autores principales: Boothe, Tobias, Hilbert, Lennart, Heide, Michael, Berninger, Lea, Huttner, Wieland B, Zaburdaev, Vasily, Vastenhouw, Nadine L, Myers, Eugene W, Drechsel, David N, Rink, Jochen C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Cell Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5582871/
https://www.ncbi.nlm.nih.gov/pubmed/28708059
http://dx.doi.org/10.7554/eLife.27240
Descripción
Sumario:In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus substantially improves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organoids. DOI: http://dx.doi.org/10.7554/eLife.27240.001

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