A tunable refractive index matching medium for live imaging cells, tissues and model organisms

In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic mediu...

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Autores principales: Boothe, Tobias, Hilbert, Lennart, Heide, Michael, Berninger, Lea, Huttner, Wieland B, Zaburdaev, Vasily, Vastenhouw, Nadine L, Myers, Eugene W, Drechsel, David N, Rink, Jochen C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Cell Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5582871/
https://www.ncbi.nlm.nih.gov/pubmed/28708059
http://dx.doi.org/10.7554/eLife.27240
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author Boothe, Tobias
Hilbert, Lennart
Heide, Michael
Berninger, Lea
Huttner, Wieland B
Zaburdaev, Vasily
Vastenhouw, Nadine L
Myers, Eugene W
Drechsel, David N
Rink, Jochen C
author_facet Boothe, Tobias
Hilbert, Lennart
Heide, Michael
Berninger, Lea
Huttner, Wieland B
Zaburdaev, Vasily
Vastenhouw, Nadine L
Myers, Eugene W
Drechsel, David N
Rink, Jochen C
author_sort Boothe, Tobias
collection PubMed
description In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus substantially improves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organoids. DOI: http://dx.doi.org/10.7554/eLife.27240.001
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spelling pubmed-55828712017-09-06 A tunable refractive index matching medium for live imaging cells, tissues and model organisms Boothe, Tobias Hilbert, Lennart Heide, Michael Berninger, Lea Huttner, Wieland B Zaburdaev, Vasily Vastenhouw, Nadine L Myers, Eugene W Drechsel, David N Rink, Jochen C eLife Cell Biology In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus substantially improves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organoids. DOI: http://dx.doi.org/10.7554/eLife.27240.001 eLife Sciences Publications, Ltd 2017-07-14 /pmc/articles/PMC5582871/ /pubmed/28708059 http://dx.doi.org/10.7554/eLife.27240 Text en © 2017, Boothe et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Boothe, Tobias
Hilbert, Lennart
Heide, Michael
Berninger, Lea
Huttner, Wieland B
Zaburdaev, Vasily
Vastenhouw, Nadine L
Myers, Eugene W
Drechsel, David N
Rink, Jochen C
A tunable refractive index matching medium for live imaging cells, tissues and model organisms
title A tunable refractive index matching medium for live imaging cells, tissues and model organisms
title_full A tunable refractive index matching medium for live imaging cells, tissues and model organisms
title_fullStr A tunable refractive index matching medium for live imaging cells, tissues and model organisms
title_full_unstemmed A tunable refractive index matching medium for live imaging cells, tissues and model organisms
title_short A tunable refractive index matching medium for live imaging cells, tissues and model organisms
title_sort tunable refractive index matching medium for live imaging cells, tissues and model organisms
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5582871/
https://www.ncbi.nlm.nih.gov/pubmed/28708059
http://dx.doi.org/10.7554/eLife.27240
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