Preliminary Characterization of MEDLE-2, a Protein Potentially Involved in the Invasion of Cryptosporidium parvum

Cryptosporidium spp. are important causes of diarrhea in humans, ruminants, and other mammals. Comparative genomic analysis indicated that genetically related and host-adapted Cryptosporidium species have different numbers of subtelomeric genes encoding the Cryptosporidium-specific MEDLE family of s...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Baoling, Wu, Haizhen, Li, Na, Su, Jiayuan, Jia, Ruilian, Jiang, Jianlin, Feng, Yaoyu, Xiao, Lihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5583231/
https://www.ncbi.nlm.nih.gov/pubmed/28912761
http://dx.doi.org/10.3389/fmicb.2017.01647
_version_ 1783261282447654912
author Li, Baoling
Wu, Haizhen
Li, Na
Su, Jiayuan
Jia, Ruilian
Jiang, Jianlin
Feng, Yaoyu
Xiao, Lihua
author_facet Li, Baoling
Wu, Haizhen
Li, Na
Su, Jiayuan
Jia, Ruilian
Jiang, Jianlin
Feng, Yaoyu
Xiao, Lihua
author_sort Li, Baoling
collection PubMed
description Cryptosporidium spp. are important causes of diarrhea in humans, ruminants, and other mammals. Comparative genomic analysis indicated that genetically related and host-adapted Cryptosporidium species have different numbers of subtelomeric genes encoding the Cryptosporidium-specific MEDLE family of secreted proteins, which could contribute to differences in host specificity. In this study, a Cryptosporidium parvum-specific member of the protein family MEDLE-2 encoded by cgd5_4590 was cloned and expressed in Escherichia coli. Immunofluorescent staining with antibodies generated from the recombinant protein showed the expression of the protein in sporozoites and development stages. In vitro neutralization assay with the antibodies partially blocked the invasion of sporozoites. These results support the potential involvement of MEDLE-2 in the invasion of host cells.
format Online
Article
Text
id pubmed-5583231
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-55832312017-09-14 Preliminary Characterization of MEDLE-2, a Protein Potentially Involved in the Invasion of Cryptosporidium parvum Li, Baoling Wu, Haizhen Li, Na Su, Jiayuan Jia, Ruilian Jiang, Jianlin Feng, Yaoyu Xiao, Lihua Front Microbiol Microbiology Cryptosporidium spp. are important causes of diarrhea in humans, ruminants, and other mammals. Comparative genomic analysis indicated that genetically related and host-adapted Cryptosporidium species have different numbers of subtelomeric genes encoding the Cryptosporidium-specific MEDLE family of secreted proteins, which could contribute to differences in host specificity. In this study, a Cryptosporidium parvum-specific member of the protein family MEDLE-2 encoded by cgd5_4590 was cloned and expressed in Escherichia coli. Immunofluorescent staining with antibodies generated from the recombinant protein showed the expression of the protein in sporozoites and development stages. In vitro neutralization assay with the antibodies partially blocked the invasion of sporozoites. These results support the potential involvement of MEDLE-2 in the invasion of host cells. Frontiers Media S.A. 2017-08-31 /pmc/articles/PMC5583231/ /pubmed/28912761 http://dx.doi.org/10.3389/fmicb.2017.01647 Text en Copyright © 2017 Li, Wu, Li, Su, Jia, Jiang, Feng and Xiao. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Baoling
Wu, Haizhen
Li, Na
Su, Jiayuan
Jia, Ruilian
Jiang, Jianlin
Feng, Yaoyu
Xiao, Lihua
Preliminary Characterization of MEDLE-2, a Protein Potentially Involved in the Invasion of Cryptosporidium parvum
title Preliminary Characterization of MEDLE-2, a Protein Potentially Involved in the Invasion of Cryptosporidium parvum
title_full Preliminary Characterization of MEDLE-2, a Protein Potentially Involved in the Invasion of Cryptosporidium parvum
title_fullStr Preliminary Characterization of MEDLE-2, a Protein Potentially Involved in the Invasion of Cryptosporidium parvum
title_full_unstemmed Preliminary Characterization of MEDLE-2, a Protein Potentially Involved in the Invasion of Cryptosporidium parvum
title_short Preliminary Characterization of MEDLE-2, a Protein Potentially Involved in the Invasion of Cryptosporidium parvum
title_sort preliminary characterization of medle-2, a protein potentially involved in the invasion of cryptosporidium parvum
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5583231/
https://www.ncbi.nlm.nih.gov/pubmed/28912761
http://dx.doi.org/10.3389/fmicb.2017.01647
work_keys_str_mv AT libaoling preliminarycharacterizationofmedle2aproteinpotentiallyinvolvedintheinvasionofcryptosporidiumparvum
AT wuhaizhen preliminarycharacterizationofmedle2aproteinpotentiallyinvolvedintheinvasionofcryptosporidiumparvum
AT lina preliminarycharacterizationofmedle2aproteinpotentiallyinvolvedintheinvasionofcryptosporidiumparvum
AT sujiayuan preliminarycharacterizationofmedle2aproteinpotentiallyinvolvedintheinvasionofcryptosporidiumparvum
AT jiaruilian preliminarycharacterizationofmedle2aproteinpotentiallyinvolvedintheinvasionofcryptosporidiumparvum
AT jiangjianlin preliminarycharacterizationofmedle2aproteinpotentiallyinvolvedintheinvasionofcryptosporidiumparvum
AT fengyaoyu preliminarycharacterizationofmedle2aproteinpotentiallyinvolvedintheinvasionofcryptosporidiumparvum
AT xiaolihua preliminarycharacterizationofmedle2aproteinpotentiallyinvolvedintheinvasionofcryptosporidiumparvum

Ejemplares similares