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Molecular and genomic features of Mycobacterium bovis strain 1595 isolated from Korean cattle

The aim of this study was to investigate the molecular characteristics and to conduct a comparative genomic analysis of Mycobacterium (M.) bovis strain 1595 isolated from a native Korean cow. Molecular typing showed that M. bovis 1595 has spoligotype SB0140 with mycobacterial interspersed repetitive...

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Autores principales: Kim, Narae, Jang, Yunho, Kim, Jin Kyoung, Ryoo, Soyoon, Kwon, Ka Hee, Kim, Miso, Kang, Shin Seok, Byeon, Hyeon Seop, Lee, Hee Soo, Lim, Young-Hee, Kim, Jae-Myung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5583421/
https://www.ncbi.nlm.nih.gov/pubmed/28385004
http://dx.doi.org/10.4142/jvs.2017.18.S1.333
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author Kim, Narae
Jang, Yunho
Kim, Jin Kyoung
Ryoo, Soyoon
Kwon, Ka Hee
Kim, Miso
Kang, Shin Seok
Byeon, Hyeon Seop
Lee, Hee Soo
Lim, Young-Hee
Kim, Jae-Myung
author_facet Kim, Narae
Jang, Yunho
Kim, Jin Kyoung
Ryoo, Soyoon
Kwon, Ka Hee
Kim, Miso
Kang, Shin Seok
Byeon, Hyeon Seop
Lee, Hee Soo
Lim, Young-Hee
Kim, Jae-Myung
author_sort Kim, Narae
collection PubMed
description The aim of this study was to investigate the molecular characteristics and to conduct a comparative genomic analysis of Mycobacterium (M.) bovis strain 1595 isolated from a native Korean cow. Molecular typing showed that M. bovis 1595 has spoligotype SB0140 with mycobacterial interspersed repetitive units-variable number of tandem repeats typing of 4-2-5-3-2-7-5-5-4-3-4-3-4-3, representing the most common type of M. bovis in Korea. The complete genome sequence of strain 1595 was determined by single-molecule real-time technology, which showed a genome of 4351712 bp in size with a 65.64% G + C content and 4358 protein-coding genes. Comparative genomic analysis with the genomes of Mycobacterium tuberculosis complex strains revealed that all genomes are similar in size and G + C content. Phylogenetic analysis revealed all strains were within a 0.1% average nucleotide identity value, and MUMmer analysis illustrated that all genomes showed positive collinearity with strain 1595. A sequence comparison based on BLASTP analysis showed that M. bovis AF2122/97 was the strain with the greatest number of completely matched proteins to M. bovis 1595. This genome sequence analysis will serve as a valuable reference for improving understanding of the virulence and epidemiologic traits among M. bovis isolates in Korea.
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spelling pubmed-55834212017-09-05 Molecular and genomic features of Mycobacterium bovis strain 1595 isolated from Korean cattle Kim, Narae Jang, Yunho Kim, Jin Kyoung Ryoo, Soyoon Kwon, Ka Hee Kim, Miso Kang, Shin Seok Byeon, Hyeon Seop Lee, Hee Soo Lim, Young-Hee Kim, Jae-Myung J Vet Sci Original Article The aim of this study was to investigate the molecular characteristics and to conduct a comparative genomic analysis of Mycobacterium (M.) bovis strain 1595 isolated from a native Korean cow. Molecular typing showed that M. bovis 1595 has spoligotype SB0140 with mycobacterial interspersed repetitive units-variable number of tandem repeats typing of 4-2-5-3-2-7-5-5-4-3-4-3-4-3, representing the most common type of M. bovis in Korea. The complete genome sequence of strain 1595 was determined by single-molecule real-time technology, which showed a genome of 4351712 bp in size with a 65.64% G + C content and 4358 protein-coding genes. Comparative genomic analysis with the genomes of Mycobacterium tuberculosis complex strains revealed that all genomes are similar in size and G + C content. Phylogenetic analysis revealed all strains were within a 0.1% average nucleotide identity value, and MUMmer analysis illustrated that all genomes showed positive collinearity with strain 1595. A sequence comparison based on BLASTP analysis showed that M. bovis AF2122/97 was the strain with the greatest number of completely matched proteins to M. bovis 1595. This genome sequence analysis will serve as a valuable reference for improving understanding of the virulence and epidemiologic traits among M. bovis isolates in Korea. The Korean Society of Veterinary Science 2017-08 2017-08-22 /pmc/articles/PMC5583421/ /pubmed/28385004 http://dx.doi.org/10.4142/jvs.2017.18.S1.333 Text en © 2017 The Korean Society of Veterinary Science http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Narae
Jang, Yunho
Kim, Jin Kyoung
Ryoo, Soyoon
Kwon, Ka Hee
Kim, Miso
Kang, Shin Seok
Byeon, Hyeon Seop
Lee, Hee Soo
Lim, Young-Hee
Kim, Jae-Myung
Molecular and genomic features of Mycobacterium bovis strain 1595 isolated from Korean cattle
title Molecular and genomic features of Mycobacterium bovis strain 1595 isolated from Korean cattle
title_full Molecular and genomic features of Mycobacterium bovis strain 1595 isolated from Korean cattle
title_fullStr Molecular and genomic features of Mycobacterium bovis strain 1595 isolated from Korean cattle
title_full_unstemmed Molecular and genomic features of Mycobacterium bovis strain 1595 isolated from Korean cattle
title_short Molecular and genomic features of Mycobacterium bovis strain 1595 isolated from Korean cattle
title_sort molecular and genomic features of mycobacterium bovis strain 1595 isolated from korean cattle
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5583421/
https://www.ncbi.nlm.nih.gov/pubmed/28385004
http://dx.doi.org/10.4142/jvs.2017.18.S1.333
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