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The Protective Effect of L-arginine in Cisplatin-induced Nephrotoxicity in Streptozotocin-induced Diabetic Rats

BACKGROUND: Cisplatin (CP) is accompanied with a nephrotoxicity. L-arginine (LA) plays an important role in the regulation of renal function. The present study was designed to investigate the protective role of LA supplementation in CP-induced nephrotoxicity in a diabetic rat's model. MATERIALS...

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Autores principales: Gharibi, Fatemeh, Soltani, Nepton, Maleki, Maryam, Talebi, Ardeshir, Nasiri, Masoumeh, Shirdavani, Soheyla, Nematbakhsh, Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5583628/
https://www.ncbi.nlm.nih.gov/pubmed/28900611
http://dx.doi.org/10.4103/2277-9175.212928
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author Gharibi, Fatemeh
Soltani, Nepton
Maleki, Maryam
Talebi, Ardeshir
Nasiri, Masoumeh
Shirdavani, Soheyla
Nematbakhsh, Mehdi
author_facet Gharibi, Fatemeh
Soltani, Nepton
Maleki, Maryam
Talebi, Ardeshir
Nasiri, Masoumeh
Shirdavani, Soheyla
Nematbakhsh, Mehdi
author_sort Gharibi, Fatemeh
collection PubMed
description BACKGROUND: Cisplatin (CP) is accompanied with a nephrotoxicity. L-arginine (LA) plays an important role in the regulation of renal function. The present study was designed to investigate the protective role of LA supplementation in CP-induced nephrotoxicity in a diabetic rat's model. MATERIALS AND METHODS: Sixteen adult female and male Wistar rats were used and they received a single dose of streptozotocin (STZ) (60 mg/kg i.p.). Diabetic female and male rats were arranged as groups 1–5 and groups 6–10, respectively. Groups 1 and 6 (LA groups) received LA alone. Groups 2 and 7 (CP groups) received CP alone. Groups 3 and 8 (CP + LA [PT] groups) received LA as prophylaxis and then treated with LA and CP. Groups 4 and 9 (CP + LA [T] groups) were treated with LA and CP simultaneously. Groups 5 and 10 (CP + LA [P] groups) received LA as prophylaxis and then treated with CP. RESULTS: The serum creatinine (Cr) level of males in Groups 8 and 9 was significantly increased when compared with LA and CP (P < 0.05), whereas no differences were observed in Cr level in female groups. Blood urea nitrogen/Cr ratio and kidney weight were reduced in all CP-receiving male rats. Such observation was not seen in female rats. Different results related to weight loss were obtained between male and female animals. The kidney tissue damage score in CP + LA (PT) male group was significantly greater than CP group (P < 0.05). CONCLUSION: Our findings indicate that administration of LA in female and male rats has no protective effect on the severity of nephrotoxicity induced by CP in diabetic rats.
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spelling pubmed-55836282017-09-12 The Protective Effect of L-arginine in Cisplatin-induced Nephrotoxicity in Streptozotocin-induced Diabetic Rats Gharibi, Fatemeh Soltani, Nepton Maleki, Maryam Talebi, Ardeshir Nasiri, Masoumeh Shirdavani, Soheyla Nematbakhsh, Mehdi Adv Biomed Res Original Article BACKGROUND: Cisplatin (CP) is accompanied with a nephrotoxicity. L-arginine (LA) plays an important role in the regulation of renal function. The present study was designed to investigate the protective role of LA supplementation in CP-induced nephrotoxicity in a diabetic rat's model. MATERIALS AND METHODS: Sixteen adult female and male Wistar rats were used and they received a single dose of streptozotocin (STZ) (60 mg/kg i.p.). Diabetic female and male rats were arranged as groups 1–5 and groups 6–10, respectively. Groups 1 and 6 (LA groups) received LA alone. Groups 2 and 7 (CP groups) received CP alone. Groups 3 and 8 (CP + LA [PT] groups) received LA as prophylaxis and then treated with LA and CP. Groups 4 and 9 (CP + LA [T] groups) were treated with LA and CP simultaneously. Groups 5 and 10 (CP + LA [P] groups) received LA as prophylaxis and then treated with CP. RESULTS: The serum creatinine (Cr) level of males in Groups 8 and 9 was significantly increased when compared with LA and CP (P < 0.05), whereas no differences were observed in Cr level in female groups. Blood urea nitrogen/Cr ratio and kidney weight were reduced in all CP-receiving male rats. Such observation was not seen in female rats. Different results related to weight loss were obtained between male and female animals. The kidney tissue damage score in CP + LA (PT) male group was significantly greater than CP group (P < 0.05). CONCLUSION: Our findings indicate that administration of LA in female and male rats has no protective effect on the severity of nephrotoxicity induced by CP in diabetic rats. Medknow Publications & Media Pvt Ltd 2017-08-14 /pmc/articles/PMC5583628/ /pubmed/28900611 http://dx.doi.org/10.4103/2277-9175.212928 Text en Copyright: © 2017 Advanced Biomedical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Gharibi, Fatemeh
Soltani, Nepton
Maleki, Maryam
Talebi, Ardeshir
Nasiri, Masoumeh
Shirdavani, Soheyla
Nematbakhsh, Mehdi
The Protective Effect of L-arginine in Cisplatin-induced Nephrotoxicity in Streptozotocin-induced Diabetic Rats
title The Protective Effect of L-arginine in Cisplatin-induced Nephrotoxicity in Streptozotocin-induced Diabetic Rats
title_full The Protective Effect of L-arginine in Cisplatin-induced Nephrotoxicity in Streptozotocin-induced Diabetic Rats
title_fullStr The Protective Effect of L-arginine in Cisplatin-induced Nephrotoxicity in Streptozotocin-induced Diabetic Rats
title_full_unstemmed The Protective Effect of L-arginine in Cisplatin-induced Nephrotoxicity in Streptozotocin-induced Diabetic Rats
title_short The Protective Effect of L-arginine in Cisplatin-induced Nephrotoxicity in Streptozotocin-induced Diabetic Rats
title_sort protective effect of l-arginine in cisplatin-induced nephrotoxicity in streptozotocin-induced diabetic rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5583628/
https://www.ncbi.nlm.nih.gov/pubmed/28900611
http://dx.doi.org/10.4103/2277-9175.212928
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