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Development and validation of an impedance biosensor for point-of-care detection of vascular cell adhesion molecule-1 toward lupus diagnostics

AIM: Systemic lupus erythematosus is an autoimmune disease that requires chronic monitoring. In this study, we demonstrate a proof-of-concept study of a highly attractive noninvasive strategy for monitoring systemic lupus erythematosus through biomarker quantification. RESULTS: This sensor technolog...

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Autores principales: Selvam, Anjan Panneer, Wangzhou, Andi, Jacobs, Michael, Wu, Tianfu, Mohan, Chandra, Prasad, Shalini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5583674/
https://www.ncbi.nlm.nih.gov/pubmed/28884017
http://dx.doi.org/10.4155/fsoa-2017-0047
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author Selvam, Anjan Panneer
Wangzhou, Andi
Jacobs, Michael
Wu, Tianfu
Mohan, Chandra
Prasad, Shalini
author_facet Selvam, Anjan Panneer
Wangzhou, Andi
Jacobs, Michael
Wu, Tianfu
Mohan, Chandra
Prasad, Shalini
author_sort Selvam, Anjan Panneer
collection PubMed
description AIM: Systemic lupus erythematosus is an autoimmune disease that requires chronic monitoring. In this study, we demonstrate a proof-of-concept study of a highly attractive noninvasive strategy for monitoring systemic lupus erythematosus through biomarker quantification. RESULTS: This sensor technology requires 50 μl of urine to detect and quantify vascular cell adhesion molecule-1 in 15 min. The sensor used nonfaradaic detection to demonstrate performance with and without detection antibody. Binding of immunoassay and target biomarkers were quantified with an impedance electrical immunoassay and correlated with an equivalent circuit. CONCLUSION: The novel sensor technology demonstrates detection in the range of 8 fg/ml to 800 pg/ml and comparative analysis with ELISA platforms was performed for 12 patient urine samples.
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spelling pubmed-55836742017-09-07 Development and validation of an impedance biosensor for point-of-care detection of vascular cell adhesion molecule-1 toward lupus diagnostics Selvam, Anjan Panneer Wangzhou, Andi Jacobs, Michael Wu, Tianfu Mohan, Chandra Prasad, Shalini Future Sci OA Research Article AIM: Systemic lupus erythematosus is an autoimmune disease that requires chronic monitoring. In this study, we demonstrate a proof-of-concept study of a highly attractive noninvasive strategy for monitoring systemic lupus erythematosus through biomarker quantification. RESULTS: This sensor technology requires 50 μl of urine to detect and quantify vascular cell adhesion molecule-1 in 15 min. The sensor used nonfaradaic detection to demonstrate performance with and without detection antibody. Binding of immunoassay and target biomarkers were quantified with an impedance electrical immunoassay and correlated with an equivalent circuit. CONCLUSION: The novel sensor technology demonstrates detection in the range of 8 fg/ml to 800 pg/ml and comparative analysis with ELISA platforms was performed for 12 patient urine samples. Future Science Ltd 2017-07-07 /pmc/articles/PMC5583674/ /pubmed/28884017 http://dx.doi.org/10.4155/fsoa-2017-0047 Text en © Shalini Prasad This work is licensed under a Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/)
spellingShingle Research Article
Selvam, Anjan Panneer
Wangzhou, Andi
Jacobs, Michael
Wu, Tianfu
Mohan, Chandra
Prasad, Shalini
Development and validation of an impedance biosensor for point-of-care detection of vascular cell adhesion molecule-1 toward lupus diagnostics
title Development and validation of an impedance biosensor for point-of-care detection of vascular cell adhesion molecule-1 toward lupus diagnostics
title_full Development and validation of an impedance biosensor for point-of-care detection of vascular cell adhesion molecule-1 toward lupus diagnostics
title_fullStr Development and validation of an impedance biosensor for point-of-care detection of vascular cell adhesion molecule-1 toward lupus diagnostics
title_full_unstemmed Development and validation of an impedance biosensor for point-of-care detection of vascular cell adhesion molecule-1 toward lupus diagnostics
title_short Development and validation of an impedance biosensor for point-of-care detection of vascular cell adhesion molecule-1 toward lupus diagnostics
title_sort development and validation of an impedance biosensor for point-of-care detection of vascular cell adhesion molecule-1 toward lupus diagnostics
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5583674/
https://www.ncbi.nlm.nih.gov/pubmed/28884017
http://dx.doi.org/10.4155/fsoa-2017-0047
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