Cargando…

Can an inadequate cervical cytology sample in ThinPrep be converted to a satisfactory sample by processing it with a SurePath preparation?

BACKGROUND: The Norwegian Cervical Cancer Screening Program recommends screening every 3 years for women between 25 and 69 years of age. There is a large difference in the percentage of unsatisfactory samples between laboratories that use different brands of liquid-based cytology. We wished to exami...

Descripción completa

Detalles Bibliográficos
Autores principales: Sørbye, Sveinung Wergeland, Pedersen, Mette Kristin, Ekeberg, Bente, Williams, Merete E. Johansen, Sauer, Torill, Chen, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5583701/
https://www.ncbi.nlm.nih.gov/pubmed/28900466
http://dx.doi.org/10.4103/cytojournal.cytojournal_34_16
_version_ 1783261367572103168
author Sørbye, Sveinung Wergeland
Pedersen, Mette Kristin
Ekeberg, Bente
Williams, Merete E. Johansen
Sauer, Torill
Chen, Ying
author_facet Sørbye, Sveinung Wergeland
Pedersen, Mette Kristin
Ekeberg, Bente
Williams, Merete E. Johansen
Sauer, Torill
Chen, Ying
author_sort Sørbye, Sveinung Wergeland
collection PubMed
description BACKGROUND: The Norwegian Cervical Cancer Screening Program recommends screening every 3 years for women between 25 and 69 years of age. There is a large difference in the percentage of unsatisfactory samples between laboratories that use different brands of liquid-based cytology. We wished to examine if inadequate ThinPrep samples could be satisfactory by processing them with the SurePath protocol. MATERIALS AND METHODS: A total of 187 inadequate ThinPrep specimens from the Department of Clinical Pathology at University Hospital of North Norway were sent to Akershus University Hospital for conversion to SurePath medium. Ninety-one (48.7%) were processed through the automated “gynecologic” application for cervix cytology samples, and 96 (51.3%) were processed with the “nongynecological” automatic program. RESULTS: Out of 187 samples that had been unsatisfactory by ThinPrep, 93 (49.7%) were satisfactory after being converted to SurePath. The rate of satisfactory cytology was 36.6% and 62.5% for samples run through the “gynecology” program and “nongynecology” program, respectively. Of the 93 samples that became satisfactory after conversion from ThinPrep to SurePath, 80 (86.0%) were screened as normal while 13 samples (14.0%) were given an abnormal diagnosis, which included 5 atypical squamous cells of undetermined significance, 5 low-grade squamous intraepithelial lesion, 2 atypical glandular cells not otherwise specified, and 1 atypical squamous cells cannot exclude high-grade squamous intraepithelial lesion. A total of 2.1% (4/187) of the women got a diagnosis of cervical intraepithelial neoplasia 2 or higher at a later follow-up. CONCLUSIONS: Converting cytology samples from ThinPrep to SurePath processing can reduce the number of unsatisfactory samples. The samples should be run through the “nongynecology” program to ensure an adequate number of cells.
format Online
Article
Text
id pubmed-5583701
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-55837012017-09-12 Can an inadequate cervical cytology sample in ThinPrep be converted to a satisfactory sample by processing it with a SurePath preparation? Sørbye, Sveinung Wergeland Pedersen, Mette Kristin Ekeberg, Bente Williams, Merete E. Johansen Sauer, Torill Chen, Ying Cytojournal Research Article BACKGROUND: The Norwegian Cervical Cancer Screening Program recommends screening every 3 years for women between 25 and 69 years of age. There is a large difference in the percentage of unsatisfactory samples between laboratories that use different brands of liquid-based cytology. We wished to examine if inadequate ThinPrep samples could be satisfactory by processing them with the SurePath protocol. MATERIALS AND METHODS: A total of 187 inadequate ThinPrep specimens from the Department of Clinical Pathology at University Hospital of North Norway were sent to Akershus University Hospital for conversion to SurePath medium. Ninety-one (48.7%) were processed through the automated “gynecologic” application for cervix cytology samples, and 96 (51.3%) were processed with the “nongynecological” automatic program. RESULTS: Out of 187 samples that had been unsatisfactory by ThinPrep, 93 (49.7%) were satisfactory after being converted to SurePath. The rate of satisfactory cytology was 36.6% and 62.5% for samples run through the “gynecology” program and “nongynecology” program, respectively. Of the 93 samples that became satisfactory after conversion from ThinPrep to SurePath, 80 (86.0%) were screened as normal while 13 samples (14.0%) were given an abnormal diagnosis, which included 5 atypical squamous cells of undetermined significance, 5 low-grade squamous intraepithelial lesion, 2 atypical glandular cells not otherwise specified, and 1 atypical squamous cells cannot exclude high-grade squamous intraepithelial lesion. A total of 2.1% (4/187) of the women got a diagnosis of cervical intraepithelial neoplasia 2 or higher at a later follow-up. CONCLUSIONS: Converting cytology samples from ThinPrep to SurePath processing can reduce the number of unsatisfactory samples. The samples should be run through the “nongynecology” program to ensure an adequate number of cells. Medknow Publications & Media Pvt Ltd 2017-08-22 /pmc/articles/PMC5583701/ /pubmed/28900466 http://dx.doi.org/10.4103/cytojournal.cytojournal_34_16 Text en Copyright: © 2017 Sørbye, et al.; Licensee Cytopathology Foundation Inc. http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Research Article
Sørbye, Sveinung Wergeland
Pedersen, Mette Kristin
Ekeberg, Bente
Williams, Merete E. Johansen
Sauer, Torill
Chen, Ying
Can an inadequate cervical cytology sample in ThinPrep be converted to a satisfactory sample by processing it with a SurePath preparation?
title Can an inadequate cervical cytology sample in ThinPrep be converted to a satisfactory sample by processing it with a SurePath preparation?
title_full Can an inadequate cervical cytology sample in ThinPrep be converted to a satisfactory sample by processing it with a SurePath preparation?
title_fullStr Can an inadequate cervical cytology sample in ThinPrep be converted to a satisfactory sample by processing it with a SurePath preparation?
title_full_unstemmed Can an inadequate cervical cytology sample in ThinPrep be converted to a satisfactory sample by processing it with a SurePath preparation?
title_short Can an inadequate cervical cytology sample in ThinPrep be converted to a satisfactory sample by processing it with a SurePath preparation?
title_sort can an inadequate cervical cytology sample in thinprep be converted to a satisfactory sample by processing it with a surepath preparation?
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5583701/
https://www.ncbi.nlm.nih.gov/pubmed/28900466
http://dx.doi.org/10.4103/cytojournal.cytojournal_34_16
work_keys_str_mv AT sørbyesveinungwergeland cananinadequatecervicalcytologysampleinthinprepbeconvertedtoasatisfactorysamplebyprocessingitwithasurepathpreparation
AT pedersenmettekristin cananinadequatecervicalcytologysampleinthinprepbeconvertedtoasatisfactorysamplebyprocessingitwithasurepathpreparation
AT ekebergbente cananinadequatecervicalcytologysampleinthinprepbeconvertedtoasatisfactorysamplebyprocessingitwithasurepathpreparation
AT williamsmereteejohansen cananinadequatecervicalcytologysampleinthinprepbeconvertedtoasatisfactorysamplebyprocessingitwithasurepathpreparation
AT sauertorill cananinadequatecervicalcytologysampleinthinprepbeconvertedtoasatisfactorysamplebyprocessingitwithasurepathpreparation
AT chenying cananinadequatecervicalcytologysampleinthinprepbeconvertedtoasatisfactorysamplebyprocessingitwithasurepathpreparation