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Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction

Cytokinesis in many eukaryotes involves a tension-generating actomyosin-based contractile ring. Many components of actomyosin rings turn over during contraction, although the significance of this turnover has remained enigmatic. Here, using Schizosaccharomyces japonicus, we investigate the role of t...

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Autores principales: Chew, Ting Gang, Huang, Junqi, Palani, Saravanan, Sommese, Ruth, Kamnev, Anton, Hatano, Tomoyuki, Gu, Ying, Oliferenko, Snezhana, Sivaramakrishnan, Sivaraj, Balasubramanian, Mohan K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5584170/
https://www.ncbi.nlm.nih.gov/pubmed/28655757
http://dx.doi.org/10.1083/jcb.201701104
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author Chew, Ting Gang
Huang, Junqi
Palani, Saravanan
Sommese, Ruth
Kamnev, Anton
Hatano, Tomoyuki
Gu, Ying
Oliferenko, Snezhana
Sivaramakrishnan, Sivaraj
Balasubramanian, Mohan K.
author_facet Chew, Ting Gang
Huang, Junqi
Palani, Saravanan
Sommese, Ruth
Kamnev, Anton
Hatano, Tomoyuki
Gu, Ying
Oliferenko, Snezhana
Sivaramakrishnan, Sivaraj
Balasubramanian, Mohan K.
author_sort Chew, Ting Gang
collection PubMed
description Cytokinesis in many eukaryotes involves a tension-generating actomyosin-based contractile ring. Many components of actomyosin rings turn over during contraction, although the significance of this turnover has remained enigmatic. Here, using Schizosaccharomyces japonicus, we investigate the role of turnover of actin and myosin II in its contraction. Actomyosin ring components self-organize into ∼1-µm-spaced clusters instead of undergoing full-ring contraction in the absence of continuous actin polymerization. This effect is reversed when actin filaments are stabilized. We tested the idea that the function of turnover is to ensure actin filament homeostasis in a synthetic system, in which we abolished turnover by fixing rings in cell ghosts with formaldehyde. We found that these rings contracted fully upon exogenous addition of a vertebrate myosin. We conclude that actin turnover is required to maintain actin filament homeostasis during ring contraction and that the requirement for turnover can be bypassed if homeostasis is achieved artificially.
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spelling pubmed-55841702017-09-10 Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction Chew, Ting Gang Huang, Junqi Palani, Saravanan Sommese, Ruth Kamnev, Anton Hatano, Tomoyuki Gu, Ying Oliferenko, Snezhana Sivaramakrishnan, Sivaraj Balasubramanian, Mohan K. J Cell Biol Research Articles Cytokinesis in many eukaryotes involves a tension-generating actomyosin-based contractile ring. Many components of actomyosin rings turn over during contraction, although the significance of this turnover has remained enigmatic. Here, using Schizosaccharomyces japonicus, we investigate the role of turnover of actin and myosin II in its contraction. Actomyosin ring components self-organize into ∼1-µm-spaced clusters instead of undergoing full-ring contraction in the absence of continuous actin polymerization. This effect is reversed when actin filaments are stabilized. We tested the idea that the function of turnover is to ensure actin filament homeostasis in a synthetic system, in which we abolished turnover by fixing rings in cell ghosts with formaldehyde. We found that these rings contracted fully upon exogenous addition of a vertebrate myosin. We conclude that actin turnover is required to maintain actin filament homeostasis during ring contraction and that the requirement for turnover can be bypassed if homeostasis is achieved artificially. The Rockefeller University Press 2017-09-04 /pmc/articles/PMC5584170/ /pubmed/28655757 http://dx.doi.org/10.1083/jcb.201701104 Text en © 2017 Chew et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Chew, Ting Gang
Huang, Junqi
Palani, Saravanan
Sommese, Ruth
Kamnev, Anton
Hatano, Tomoyuki
Gu, Ying
Oliferenko, Snezhana
Sivaramakrishnan, Sivaraj
Balasubramanian, Mohan K.
Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction
title Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction
title_full Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction
title_fullStr Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction
title_full_unstemmed Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction
title_short Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction
title_sort actin turnover maintains actin filament homeostasis during cytokinetic ring contraction
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5584170/
https://www.ncbi.nlm.nih.gov/pubmed/28655757
http://dx.doi.org/10.1083/jcb.201701104
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