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Effects of increased Kindlin-2 expression in bladder cancer stromal fibroblasts
Kindlin-2 is a focal adhesion protein highly expressed in bladder cancer stromal fibroblasts. We investigated the prognostic significance of Kindlin-2 in bladder cancer stromal fibroblasts and evaluated the effects of Kindlin-2 on the malignant behaviors of tumor cells. Immunohistochemical staining...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5584191/ https://www.ncbi.nlm.nih.gov/pubmed/28881595 http://dx.doi.org/10.18632/oncotarget.17021 |
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author | Wu, Jitao Yu, Cuicui Cai, Li Lu, Youyi Jiang, Lei Liu, Chu Li, Yongwei Feng, Fan Gao, Zhenli Zhu, Zhe Yu, Shengqiang Yuan, Hejia Cui, Yuanshan |
author_facet | Wu, Jitao Yu, Cuicui Cai, Li Lu, Youyi Jiang, Lei Liu, Chu Li, Yongwei Feng, Fan Gao, Zhenli Zhu, Zhe Yu, Shengqiang Yuan, Hejia Cui, Yuanshan |
author_sort | Wu, Jitao |
collection | PubMed |
description | Kindlin-2 is a focal adhesion protein highly expressed in bladder cancer stromal fibroblasts. We investigated the prognostic significance of Kindlin-2 in bladder cancer stromal fibroblasts and evaluated the effects of Kindlin-2 on the malignant behaviors of tumor cells. Immunohistochemical staining of 203 paraffin-embedded bladder cancer tissues showed that Kindlin-2 expression correlated with advanced stage, high grade, and relapse of bladder cancer. Kaplan-Meier survival analysis demonstrated that patients exhibiting high Kindlin-2 expression had shorter survival times than those with low Kindlin-2 expression (p < 0.01). Multivariate analysis revealed that high Kindlin-2 expression leads to poor prognosis in bladder cancer. Using cancer-associated fibroblasts (CAFs) isolated from human bladder cancer tissue, we observed that Kindlin-2 knockdown decreased CAFs activation, resulting in decreased expression of α-smooth muscle actin (α-SMA) and the extracellular matrix protein fibronectin. Kindlin-2 suppression also reduced CAF-induced bladder cancer cell migration and invasion. Moreover, we found that Kindlin-2 activates CAFs and promotes the invasiveness of bladder cancer cells by stimulating TGF-β-induced epithelial-mesenchymal transition. These results support targeting Kindlin-2 and the corresponding activated CAFs in bladder cancer therapy. |
format | Online Article Text |
id | pubmed-5584191 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-55841912017-09-06 Effects of increased Kindlin-2 expression in bladder cancer stromal fibroblasts Wu, Jitao Yu, Cuicui Cai, Li Lu, Youyi Jiang, Lei Liu, Chu Li, Yongwei Feng, Fan Gao, Zhenli Zhu, Zhe Yu, Shengqiang Yuan, Hejia Cui, Yuanshan Oncotarget Research Paper Kindlin-2 is a focal adhesion protein highly expressed in bladder cancer stromal fibroblasts. We investigated the prognostic significance of Kindlin-2 in bladder cancer stromal fibroblasts and evaluated the effects of Kindlin-2 on the malignant behaviors of tumor cells. Immunohistochemical staining of 203 paraffin-embedded bladder cancer tissues showed that Kindlin-2 expression correlated with advanced stage, high grade, and relapse of bladder cancer. Kaplan-Meier survival analysis demonstrated that patients exhibiting high Kindlin-2 expression had shorter survival times than those with low Kindlin-2 expression (p < 0.01). Multivariate analysis revealed that high Kindlin-2 expression leads to poor prognosis in bladder cancer. Using cancer-associated fibroblasts (CAFs) isolated from human bladder cancer tissue, we observed that Kindlin-2 knockdown decreased CAFs activation, resulting in decreased expression of α-smooth muscle actin (α-SMA) and the extracellular matrix protein fibronectin. Kindlin-2 suppression also reduced CAF-induced bladder cancer cell migration and invasion. Moreover, we found that Kindlin-2 activates CAFs and promotes the invasiveness of bladder cancer cells by stimulating TGF-β-induced epithelial-mesenchymal transition. These results support targeting Kindlin-2 and the corresponding activated CAFs in bladder cancer therapy. Impact Journals LLC 2017-04-10 /pmc/articles/PMC5584191/ /pubmed/28881595 http://dx.doi.org/10.18632/oncotarget.17021 Text en Copyright: © 2017 Wu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Wu, Jitao Yu, Cuicui Cai, Li Lu, Youyi Jiang, Lei Liu, Chu Li, Yongwei Feng, Fan Gao, Zhenli Zhu, Zhe Yu, Shengqiang Yuan, Hejia Cui, Yuanshan Effects of increased Kindlin-2 expression in bladder cancer stromal fibroblasts |
title | Effects of increased Kindlin-2 expression in bladder cancer stromal fibroblasts |
title_full | Effects of increased Kindlin-2 expression in bladder cancer stromal fibroblasts |
title_fullStr | Effects of increased Kindlin-2 expression in bladder cancer stromal fibroblasts |
title_full_unstemmed | Effects of increased Kindlin-2 expression in bladder cancer stromal fibroblasts |
title_short | Effects of increased Kindlin-2 expression in bladder cancer stromal fibroblasts |
title_sort | effects of increased kindlin-2 expression in bladder cancer stromal fibroblasts |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5584191/ https://www.ncbi.nlm.nih.gov/pubmed/28881595 http://dx.doi.org/10.18632/oncotarget.17021 |
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