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TGFbeta and miRNA regulation in familial and sporadic breast cancer

The term ‘BRCAness’ was introduced to identify sporadic malignant tumors sharing characteristics similar to those germline BRCA-related. Among all mechanisms attributable to BRCA1 expression silencing, a major role has been assigned to microRNAs. MicroRNAs role in familial and sporadic breast cancer...

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Autores principales: Danza, Katia, De Summa, Simona, Pinto, Rosamaria, Pilato, Brunella, Palumbo, Orazio, Carella, Massimo, Popescu, Ondina, Digennaro, Maria, Lacalamita, Rosanna, Tommasi, Stefania
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5584195/
https://www.ncbi.nlm.nih.gov/pubmed/28881597
http://dx.doi.org/10.18632/oncotarget.14899
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author Danza, Katia
De Summa, Simona
Pinto, Rosamaria
Pilato, Brunella
Palumbo, Orazio
Carella, Massimo
Popescu, Ondina
Digennaro, Maria
Lacalamita, Rosanna
Tommasi, Stefania
author_facet Danza, Katia
De Summa, Simona
Pinto, Rosamaria
Pilato, Brunella
Palumbo, Orazio
Carella, Massimo
Popescu, Ondina
Digennaro, Maria
Lacalamita, Rosanna
Tommasi, Stefania
author_sort Danza, Katia
collection PubMed
description The term ‘BRCAness’ was introduced to identify sporadic malignant tumors sharing characteristics similar to those germline BRCA-related. Among all mechanisms attributable to BRCA1 expression silencing, a major role has been assigned to microRNAs. MicroRNAs role in familial and sporadic breast cancer has been explored but few data are available about microRNAs involvement in homologous recombination repair control in these breast cancer subgroups. Our aim was to seek microRNAs associated to pathways underlying DNA repair dysfunction in breast cancer according to a family history of the disease. Affymetrix GeneChip microRNA Arrays were used to perform microRNA expression analysis in familial and sporadic breast cancer. Pathway enrichment analysis and microRNA target prediction was carried out using DIANA miRPath v.3 web-based computational tool and miRWalk v.2 database. We analyzed an external gene expression dataset (E-GEOD-49481), including both familial and sporadic breast cancers. For microRNA validation, an independent set of 19 familial and 10 sporadic breast cancers was used. Microarray analysis identified a signature of 28 deregulated miRNAs. For our validation analyses by real time PCR, we focused on miR-92a-1*, miR-1184 and miR-943 because associated to TGF-β signalling pathway, ATM and BRCA1 genes expression. Our results highlighted alterations in miR-92a-1*, miR-1184 and miR-943 expression levels suggesting their involvement in repair of DNA double-strand breaks through TGF-beta pathway control.
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spelling pubmed-55841952017-09-06 TGFbeta and miRNA regulation in familial and sporadic breast cancer Danza, Katia De Summa, Simona Pinto, Rosamaria Pilato, Brunella Palumbo, Orazio Carella, Massimo Popescu, Ondina Digennaro, Maria Lacalamita, Rosanna Tommasi, Stefania Oncotarget Research Paper The term ‘BRCAness’ was introduced to identify sporadic malignant tumors sharing characteristics similar to those germline BRCA-related. Among all mechanisms attributable to BRCA1 expression silencing, a major role has been assigned to microRNAs. MicroRNAs role in familial and sporadic breast cancer has been explored but few data are available about microRNAs involvement in homologous recombination repair control in these breast cancer subgroups. Our aim was to seek microRNAs associated to pathways underlying DNA repair dysfunction in breast cancer according to a family history of the disease. Affymetrix GeneChip microRNA Arrays were used to perform microRNA expression analysis in familial and sporadic breast cancer. Pathway enrichment analysis and microRNA target prediction was carried out using DIANA miRPath v.3 web-based computational tool and miRWalk v.2 database. We analyzed an external gene expression dataset (E-GEOD-49481), including both familial and sporadic breast cancers. For microRNA validation, an independent set of 19 familial and 10 sporadic breast cancers was used. Microarray analysis identified a signature of 28 deregulated miRNAs. For our validation analyses by real time PCR, we focused on miR-92a-1*, miR-1184 and miR-943 because associated to TGF-β signalling pathway, ATM and BRCA1 genes expression. Our results highlighted alterations in miR-92a-1*, miR-1184 and miR-943 expression levels suggesting their involvement in repair of DNA double-strand breaks through TGF-beta pathway control. Impact Journals LLC 2017-01-30 /pmc/articles/PMC5584195/ /pubmed/28881597 http://dx.doi.org/10.18632/oncotarget.14899 Text en Copyright: © 2017 Danza et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Danza, Katia
De Summa, Simona
Pinto, Rosamaria
Pilato, Brunella
Palumbo, Orazio
Carella, Massimo
Popescu, Ondina
Digennaro, Maria
Lacalamita, Rosanna
Tommasi, Stefania
TGFbeta and miRNA regulation in familial and sporadic breast cancer
title TGFbeta and miRNA regulation in familial and sporadic breast cancer
title_full TGFbeta and miRNA regulation in familial and sporadic breast cancer
title_fullStr TGFbeta and miRNA regulation in familial and sporadic breast cancer
title_full_unstemmed TGFbeta and miRNA regulation in familial and sporadic breast cancer
title_short TGFbeta and miRNA regulation in familial and sporadic breast cancer
title_sort tgfbeta and mirna regulation in familial and sporadic breast cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5584195/
https://www.ncbi.nlm.nih.gov/pubmed/28881597
http://dx.doi.org/10.18632/oncotarget.14899
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