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In vitro asymbiotic germination for micropropagation of the recalcitrant terrestrial orchid Chloraea crispa (Orchidaceae)(1)

PREMISE OF THE STUDY: Chloraea crispa is a terrestrial Orchidaceae species native to Chile, characterized by a beautiful and showy inflorescence. The species has a great potential for commercial exploitation in the cut flower industry, but it is essential to improve propagation methods to avoid enda...

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Autores principales: Quiroz, Karla, Saavedra, Jessica, Vogel, Hermine, Verdugo, Gabriela, Caligari, Peter D. S., García-Gonzáles, Rolando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5584813/
https://www.ncbi.nlm.nih.gov/pubmed/28924509
http://dx.doi.org/10.3732/apps.1600142
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author Quiroz, Karla
Saavedra, Jessica
Vogel, Hermine
Verdugo, Gabriela
Caligari, Peter D. S.
García-Gonzáles, Rolando
author_facet Quiroz, Karla
Saavedra, Jessica
Vogel, Hermine
Verdugo, Gabriela
Caligari, Peter D. S.
García-Gonzáles, Rolando
author_sort Quiroz, Karla
collection PubMed
description PREMISE OF THE STUDY: Chloraea crispa is a terrestrial Orchidaceae species native to Chile, characterized by a beautiful and showy inflorescence. The species has a great potential for commercial exploitation in the cut flower industry, but it is essential to improve propagation methods to avoid endangering its natural populations. Because this species is hard to propagate using traditional greenhouse techniques, in vitro techniques offer an effective tool for its large-scale production in terms of germination, growth, and propagation. METHODS: The current study evaluated the effect of the culture medium on the asymbiotic germination of C. crispa seeds, as well as the effects of the plant growth regulators 6-benzylaminopurine and indole-3-butyric acid. Different light regimes were also studied. RESULTS: A significant effect was observed for the interaction between culture media and light regime on the morphogenic response of the seeds. The highest rate of embryonic germination was obtained in Van Waes medium supplemented with 0.1 mg·L(−1) of 6-benzylaminopurine. DISCUSSION: For the first time, asymbiotic culture of this species using biotechnology tools has been developed. Plantlets developed very well under in vitro conditions, allowing the possibility to propagate and store genetic material for conservation and domestication purposes.
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spelling pubmed-55848132017-09-18 In vitro asymbiotic germination for micropropagation of the recalcitrant terrestrial orchid Chloraea crispa (Orchidaceae)(1) Quiroz, Karla Saavedra, Jessica Vogel, Hermine Verdugo, Gabriela Caligari, Peter D. S. García-Gonzáles, Rolando Appl Plant Sci Application Article PREMISE OF THE STUDY: Chloraea crispa is a terrestrial Orchidaceae species native to Chile, characterized by a beautiful and showy inflorescence. The species has a great potential for commercial exploitation in the cut flower industry, but it is essential to improve propagation methods to avoid endangering its natural populations. Because this species is hard to propagate using traditional greenhouse techniques, in vitro techniques offer an effective tool for its large-scale production in terms of germination, growth, and propagation. METHODS: The current study evaluated the effect of the culture medium on the asymbiotic germination of C. crispa seeds, as well as the effects of the plant growth regulators 6-benzylaminopurine and indole-3-butyric acid. Different light regimes were also studied. RESULTS: A significant effect was observed for the interaction between culture media and light regime on the morphogenic response of the seeds. The highest rate of embryonic germination was obtained in Van Waes medium supplemented with 0.1 mg·L(−1) of 6-benzylaminopurine. DISCUSSION: For the first time, asymbiotic culture of this species using biotechnology tools has been developed. Plantlets developed very well under in vitro conditions, allowing the possibility to propagate and store genetic material for conservation and domestication purposes. Botanical Society of America 2017-08-29 /pmc/articles/PMC5584813/ /pubmed/28924509 http://dx.doi.org/10.3732/apps.1600142 Text en © 2017 Quiroz et al. Published by the Botanical Society of America https://creativecommons.org/licenses/by-nc-sa/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC-SA 4.0 (https://creativecommons.org/licenses/by-nc-sa/4.0/) ), which permits unrestricted noncommercial use and redistribution provided that the original author and source are credited and the new work is distributed under the same license as the original.
spellingShingle Application Article
Quiroz, Karla
Saavedra, Jessica
Vogel, Hermine
Verdugo, Gabriela
Caligari, Peter D. S.
García-Gonzáles, Rolando
In vitro asymbiotic germination for micropropagation of the recalcitrant terrestrial orchid Chloraea crispa (Orchidaceae)(1)
title In vitro asymbiotic germination for micropropagation of the recalcitrant terrestrial orchid Chloraea crispa (Orchidaceae)(1)
title_full In vitro asymbiotic germination for micropropagation of the recalcitrant terrestrial orchid Chloraea crispa (Orchidaceae)(1)
title_fullStr In vitro asymbiotic germination for micropropagation of the recalcitrant terrestrial orchid Chloraea crispa (Orchidaceae)(1)
title_full_unstemmed In vitro asymbiotic germination for micropropagation of the recalcitrant terrestrial orchid Chloraea crispa (Orchidaceae)(1)
title_short In vitro asymbiotic germination for micropropagation of the recalcitrant terrestrial orchid Chloraea crispa (Orchidaceae)(1)
title_sort in vitro asymbiotic germination for micropropagation of the recalcitrant terrestrial orchid chloraea crispa (orchidaceae)(1)
topic Application Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5584813/
https://www.ncbi.nlm.nih.gov/pubmed/28924509
http://dx.doi.org/10.3732/apps.1600142
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