Fourteen polymorphic microsatellite markers for a widespread limestone endemic, Carex eburnea (Cyperaceae: Carex sect. Albae)(1)

PREMISE OF THE STUDY: Microsatellite primers were developed for a widespread limestone endemic sedge, Carex eburnea, to facilitate investigation of the genetic diversity and phylogeography of this taxon and its closest relative, C. mckittrickensis. METHODS AND RESULTS: Forty-eight primer pairs were...

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Autores principales: Gillespie, Emily L., Pauley, Annabella G., Haffner, Megan L., Hay, Nikolai M., Estep, Matt C., Murrell, Zack E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2017
Materias:
Primer Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5584816/
https://www.ncbi.nlm.nih.gov/pubmed/28924512
http://dx.doi.org/10.3732/apps.1700031
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author Gillespie, Emily L.
Pauley, Annabella G.
Haffner, Megan L.
Hay, Nikolai M.
Estep, Matt C.
Murrell, Zack E.
author_facet Gillespie, Emily L.
Pauley, Annabella G.
Haffner, Megan L.
Hay, Nikolai M.
Estep, Matt C.
Murrell, Zack E.
author_sort Gillespie, Emily L.
collection PubMed
description PREMISE OF THE STUDY: Microsatellite primers were developed for a widespread limestone endemic sedge, Carex eburnea, to facilitate investigation of the genetic diversity and phylogeography of this taxon and its closest relative, C. mckittrickensis. METHODS AND RESULTS: Forty-eight primer pairs were designed from Illumina sequence data and screened for suitability. Fourteen of these primer pairs were polymorphic and generated one to seven alleles per locus. Cross-species amplifications were conducted for all four members of Carex sect. Albae. CONCLUSIONS: These primer pairs can be used to assess the genetic diversity and population structure in future studies of C. eburnea and C. mckittrickensis, and likely in other members of Carex sect. Albae.
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spelling pubmed-55848162017-09-18 Fourteen polymorphic microsatellite markers for a widespread limestone endemic, Carex eburnea (Cyperaceae: Carex sect. Albae)(1) Gillespie, Emily L. Pauley, Annabella G. Haffner, Megan L. Hay, Nikolai M. Estep, Matt C. Murrell, Zack E. Appl Plant Sci Primer Note PREMISE OF THE STUDY: Microsatellite primers were developed for a widespread limestone endemic sedge, Carex eburnea, to facilitate investigation of the genetic diversity and phylogeography of this taxon and its closest relative, C. mckittrickensis. METHODS AND RESULTS: Forty-eight primer pairs were designed from Illumina sequence data and screened for suitability. Fourteen of these primer pairs were polymorphic and generated one to seven alleles per locus. Cross-species amplifications were conducted for all four members of Carex sect. Albae. CONCLUSIONS: These primer pairs can be used to assess the genetic diversity and population structure in future studies of C. eburnea and C. mckittrickensis, and likely in other members of Carex sect. Albae. Botanical Society of America 2017-08-21 /pmc/articles/PMC5584816/ /pubmed/28924512 http://dx.doi.org/10.3732/apps.1700031 Text en © 2017 Gillespie et al. Published by the Botanical Society of America https://creativecommons.org/licenses/by-nc-sa/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC-SA 4.0 (https://creativecommons.org/licenses/by-nc-sa/4.0/) ), which permits unrestricted noncommercial use and redistribution provided that the original author and source are credited and the new work is distributed under the same license as the original.
spellingShingle Primer Note
Gillespie, Emily L.
Pauley, Annabella G.
Haffner, Megan L.
Hay, Nikolai M.
Estep, Matt C.
Murrell, Zack E.
Fourteen polymorphic microsatellite markers for a widespread limestone endemic, Carex eburnea (Cyperaceae: Carex sect. Albae)(1)
title Fourteen polymorphic microsatellite markers for a widespread limestone endemic, Carex eburnea (Cyperaceae: Carex sect. Albae)(1)
title_full Fourteen polymorphic microsatellite markers for a widespread limestone endemic, Carex eburnea (Cyperaceae: Carex sect. Albae)(1)
title_fullStr Fourteen polymorphic microsatellite markers for a widespread limestone endemic, Carex eburnea (Cyperaceae: Carex sect. Albae)(1)
title_full_unstemmed Fourteen polymorphic microsatellite markers for a widespread limestone endemic, Carex eburnea (Cyperaceae: Carex sect. Albae)(1)
title_short Fourteen polymorphic microsatellite markers for a widespread limestone endemic, Carex eburnea (Cyperaceae: Carex sect. Albae)(1)
title_sort fourteen polymorphic microsatellite markers for a widespread limestone endemic, carex eburnea (cyperaceae: carex sect. albae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5584816/
https://www.ncbi.nlm.nih.gov/pubmed/28924512
http://dx.doi.org/10.3732/apps.1700031
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