Cargando…
Heterotrimeric G Protein-Regulated Ca(2+) Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP
Extracellular ATP (eATP) has been reported to be involved in plant growth as a primary messenger in the apoplast. Here, roots of Arabidopsis thaliana seedlings growing in jointed medium bent upon contact with ATP-containing medium to keep away from eATP, showing a marked avoidance response. Roots re...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585194/ https://www.ncbi.nlm.nih.gov/pubmed/28919907 http://dx.doi.org/10.3389/fpls.2017.01522 |
_version_ | 1783261569925251072 |
---|---|
author | Zhu, Ruojia Dong, Xiaoxia Hao, Weiwei Gao, Wei Zhang, Wenzhu Xia, Shuyan Liu, Ting Shang, Zhonglin |
author_facet | Zhu, Ruojia Dong, Xiaoxia Hao, Weiwei Gao, Wei Zhang, Wenzhu Xia, Shuyan Liu, Ting Shang, Zhonglin |
author_sort | Zhu, Ruojia |
collection | PubMed |
description | Extracellular ATP (eATP) has been reported to be involved in plant growth as a primary messenger in the apoplast. Here, roots of Arabidopsis thaliana seedlings growing in jointed medium bent upon contact with ATP-containing medium to keep away from eATP, showing a marked avoidance response. Roots responded similarly to ADP and bz-ATP but did not respond to AMP and GTP. The eATP avoidance response was reduced in loss-of-function mutants of heterotrimeric G protein α subunit (Gα) (gpa1-1 and gpa1-2) and enhanced in Gα-over-expression (OE) lines (wGα and cGα). Ethylenebis(oxyethylenenitrilo) tetraacetic acid (EGTA) and Gd(3+) remarkably suppressed eATP-induced root bending. ATP-stimulated Ca(2+) influx was impaired in Gα null mutants and increased in its OE lines. DR5-GFP and PIN2 were asymmetrically distributed in ATP-stimulated root tips, this effect was strongly suppressed by EGTA and diminished in Gα null mutants. In addition, some eATP-induced genes' expression was also impaired in Gα null mutants. Based on these results, we propose that heterotrimeric Gα-regulated Ca(2+) influx and PIN2 distribution may be key signaling events in eATP sensing and avoidance response in Arabidopsis thaliana roots. |
format | Online Article Text |
id | pubmed-5585194 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-55851942017-09-15 Heterotrimeric G Protein-Regulated Ca(2+) Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP Zhu, Ruojia Dong, Xiaoxia Hao, Weiwei Gao, Wei Zhang, Wenzhu Xia, Shuyan Liu, Ting Shang, Zhonglin Front Plant Sci Plant Science Extracellular ATP (eATP) has been reported to be involved in plant growth as a primary messenger in the apoplast. Here, roots of Arabidopsis thaliana seedlings growing in jointed medium bent upon contact with ATP-containing medium to keep away from eATP, showing a marked avoidance response. Roots responded similarly to ADP and bz-ATP but did not respond to AMP and GTP. The eATP avoidance response was reduced in loss-of-function mutants of heterotrimeric G protein α subunit (Gα) (gpa1-1 and gpa1-2) and enhanced in Gα-over-expression (OE) lines (wGα and cGα). Ethylenebis(oxyethylenenitrilo) tetraacetic acid (EGTA) and Gd(3+) remarkably suppressed eATP-induced root bending. ATP-stimulated Ca(2+) influx was impaired in Gα null mutants and increased in its OE lines. DR5-GFP and PIN2 were asymmetrically distributed in ATP-stimulated root tips, this effect was strongly suppressed by EGTA and diminished in Gα null mutants. In addition, some eATP-induced genes' expression was also impaired in Gα null mutants. Based on these results, we propose that heterotrimeric Gα-regulated Ca(2+) influx and PIN2 distribution may be key signaling events in eATP sensing and avoidance response in Arabidopsis thaliana roots. Frontiers Media S.A. 2017-09-01 /pmc/articles/PMC5585194/ /pubmed/28919907 http://dx.doi.org/10.3389/fpls.2017.01522 Text en Copyright © 2017 Zhu, Dong, Hao, Gao, Zhang, Xia, Liu and Shang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Zhu, Ruojia Dong, Xiaoxia Hao, Weiwei Gao, Wei Zhang, Wenzhu Xia, Shuyan Liu, Ting Shang, Zhonglin Heterotrimeric G Protein-Regulated Ca(2+) Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP |
title | Heterotrimeric G Protein-Regulated Ca(2+) Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP |
title_full | Heterotrimeric G Protein-Regulated Ca(2+) Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP |
title_fullStr | Heterotrimeric G Protein-Regulated Ca(2+) Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP |
title_full_unstemmed | Heterotrimeric G Protein-Regulated Ca(2+) Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP |
title_short | Heterotrimeric G Protein-Regulated Ca(2+) Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP |
title_sort | heterotrimeric g protein-regulated ca(2+) influx and pin2 asymmetric distribution are involved in arabidopsis thaliana roots' avoidance response to extracellular atp |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585194/ https://www.ncbi.nlm.nih.gov/pubmed/28919907 http://dx.doi.org/10.3389/fpls.2017.01522 |
work_keys_str_mv | AT zhuruojia heterotrimericgproteinregulatedca2influxandpin2asymmetricdistributionareinvolvedinarabidopsisthalianarootsavoidanceresponsetoextracellularatp AT dongxiaoxia heterotrimericgproteinregulatedca2influxandpin2asymmetricdistributionareinvolvedinarabidopsisthalianarootsavoidanceresponsetoextracellularatp AT haoweiwei heterotrimericgproteinregulatedca2influxandpin2asymmetricdistributionareinvolvedinarabidopsisthalianarootsavoidanceresponsetoextracellularatp AT gaowei heterotrimericgproteinregulatedca2influxandpin2asymmetricdistributionareinvolvedinarabidopsisthalianarootsavoidanceresponsetoextracellularatp AT zhangwenzhu heterotrimericgproteinregulatedca2influxandpin2asymmetricdistributionareinvolvedinarabidopsisthalianarootsavoidanceresponsetoextracellularatp AT xiashuyan heterotrimericgproteinregulatedca2influxandpin2asymmetricdistributionareinvolvedinarabidopsisthalianarootsavoidanceresponsetoextracellularatp AT liuting heterotrimericgproteinregulatedca2influxandpin2asymmetricdistributionareinvolvedinarabidopsisthalianarootsavoidanceresponsetoextracellularatp AT shangzhonglin heterotrimericgproteinregulatedca2influxandpin2asymmetricdistributionareinvolvedinarabidopsisthalianarootsavoidanceresponsetoextracellularatp |