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Heterotrimeric G Protein-Regulated Ca(2+) Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP

Extracellular ATP (eATP) has been reported to be involved in plant growth as a primary messenger in the apoplast. Here, roots of Arabidopsis thaliana seedlings growing in jointed medium bent upon contact with ATP-containing medium to keep away from eATP, showing a marked avoidance response. Roots re...

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Autores principales: Zhu, Ruojia, Dong, Xiaoxia, Hao, Weiwei, Gao, Wei, Zhang, Wenzhu, Xia, Shuyan, Liu, Ting, Shang, Zhonglin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585194/
https://www.ncbi.nlm.nih.gov/pubmed/28919907
http://dx.doi.org/10.3389/fpls.2017.01522
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author Zhu, Ruojia
Dong, Xiaoxia
Hao, Weiwei
Gao, Wei
Zhang, Wenzhu
Xia, Shuyan
Liu, Ting
Shang, Zhonglin
author_facet Zhu, Ruojia
Dong, Xiaoxia
Hao, Weiwei
Gao, Wei
Zhang, Wenzhu
Xia, Shuyan
Liu, Ting
Shang, Zhonglin
author_sort Zhu, Ruojia
collection PubMed
description Extracellular ATP (eATP) has been reported to be involved in plant growth as a primary messenger in the apoplast. Here, roots of Arabidopsis thaliana seedlings growing in jointed medium bent upon contact with ATP-containing medium to keep away from eATP, showing a marked avoidance response. Roots responded similarly to ADP and bz-ATP but did not respond to AMP and GTP. The eATP avoidance response was reduced in loss-of-function mutants of heterotrimeric G protein α subunit (Gα) (gpa1-1 and gpa1-2) and enhanced in Gα-over-expression (OE) lines (wGα and cGα). Ethylenebis(oxyethylenenitrilo) tetraacetic acid (EGTA) and Gd(3+) remarkably suppressed eATP-induced root bending. ATP-stimulated Ca(2+) influx was impaired in Gα null mutants and increased in its OE lines. DR5-GFP and PIN2 were asymmetrically distributed in ATP-stimulated root tips, this effect was strongly suppressed by EGTA and diminished in Gα null mutants. In addition, some eATP-induced genes' expression was also impaired in Gα null mutants. Based on these results, we propose that heterotrimeric Gα-regulated Ca(2+) influx and PIN2 distribution may be key signaling events in eATP sensing and avoidance response in Arabidopsis thaliana roots.
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spelling pubmed-55851942017-09-15 Heterotrimeric G Protein-Regulated Ca(2+) Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP Zhu, Ruojia Dong, Xiaoxia Hao, Weiwei Gao, Wei Zhang, Wenzhu Xia, Shuyan Liu, Ting Shang, Zhonglin Front Plant Sci Plant Science Extracellular ATP (eATP) has been reported to be involved in plant growth as a primary messenger in the apoplast. Here, roots of Arabidopsis thaliana seedlings growing in jointed medium bent upon contact with ATP-containing medium to keep away from eATP, showing a marked avoidance response. Roots responded similarly to ADP and bz-ATP but did not respond to AMP and GTP. The eATP avoidance response was reduced in loss-of-function mutants of heterotrimeric G protein α subunit (Gα) (gpa1-1 and gpa1-2) and enhanced in Gα-over-expression (OE) lines (wGα and cGα). Ethylenebis(oxyethylenenitrilo) tetraacetic acid (EGTA) and Gd(3+) remarkably suppressed eATP-induced root bending. ATP-stimulated Ca(2+) influx was impaired in Gα null mutants and increased in its OE lines. DR5-GFP and PIN2 were asymmetrically distributed in ATP-stimulated root tips, this effect was strongly suppressed by EGTA and diminished in Gα null mutants. In addition, some eATP-induced genes' expression was also impaired in Gα null mutants. Based on these results, we propose that heterotrimeric Gα-regulated Ca(2+) influx and PIN2 distribution may be key signaling events in eATP sensing and avoidance response in Arabidopsis thaliana roots. Frontiers Media S.A. 2017-09-01 /pmc/articles/PMC5585194/ /pubmed/28919907 http://dx.doi.org/10.3389/fpls.2017.01522 Text en Copyright © 2017 Zhu, Dong, Hao, Gao, Zhang, Xia, Liu and Shang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhu, Ruojia
Dong, Xiaoxia
Hao, Weiwei
Gao, Wei
Zhang, Wenzhu
Xia, Shuyan
Liu, Ting
Shang, Zhonglin
Heterotrimeric G Protein-Regulated Ca(2+) Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP
title Heterotrimeric G Protein-Regulated Ca(2+) Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP
title_full Heterotrimeric G Protein-Regulated Ca(2+) Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP
title_fullStr Heterotrimeric G Protein-Regulated Ca(2+) Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP
title_full_unstemmed Heterotrimeric G Protein-Regulated Ca(2+) Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP
title_short Heterotrimeric G Protein-Regulated Ca(2+) Influx and PIN2 Asymmetric Distribution Are Involved in Arabidopsis thaliana Roots' Avoidance Response to Extracellular ATP
title_sort heterotrimeric g protein-regulated ca(2+) influx and pin2 asymmetric distribution are involved in arabidopsis thaliana roots' avoidance response to extracellular atp
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585194/
https://www.ncbi.nlm.nih.gov/pubmed/28919907
http://dx.doi.org/10.3389/fpls.2017.01522
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