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Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins
Sensitivity, dynamic and detection range as well as exclusion of expression and instrumental artifacts are critical for the quantitation of data obtained with fluorescent protein (FP)-based biosensors in vivo. Current biosensors designs are, in general, unable to simultaneously meet all these criter...
Autores principales: | Ast, Cindy, Foret, Jessica, Oltrogge, Luke M., De Michele, Roberto, Kleist, Thomas J., Ho, Cheng-Hsun, Frommer, Wolf B. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585204/ https://www.ncbi.nlm.nih.gov/pubmed/28874729 http://dx.doi.org/10.1038/s41467-017-00400-2 |
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