CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs

Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in p...

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Detalles Bibliográficos
Autores principales: Wu, Jun, Vilarino, Marcela, Suzuki, Keiichiro, Okamura, Daiji, Bogliotti, Yanina Soledad, Park, Insung, Rowe, Joan, McNabb, Bret, Ross, Pablo Juan, Belmonte, Juan Carlos Izpisua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585254/
https://www.ncbi.nlm.nih.gov/pubmed/28874671
http://dx.doi.org/10.1038/s41598-017-08596-5
Descripción
Sumario:Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.

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