CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs

Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in p...

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Autores principales: Wu, Jun, Vilarino, Marcela, Suzuki, Keiichiro, Okamura, Daiji, Bogliotti, Yanina Soledad, Park, Insung, Rowe, Joan, McNabb, Bret, Ross, Pablo Juan, Belmonte, Juan Carlos Izpisua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585254/
https://www.ncbi.nlm.nih.gov/pubmed/28874671
http://dx.doi.org/10.1038/s41598-017-08596-5
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author Wu, Jun
Vilarino, Marcela
Suzuki, Keiichiro
Okamura, Daiji
Bogliotti, Yanina Soledad
Park, Insung
Rowe, Joan
McNabb, Bret
Ross, Pablo Juan
Belmonte, Juan Carlos Izpisua
author_facet Wu, Jun
Vilarino, Marcela
Suzuki, Keiichiro
Okamura, Daiji
Bogliotti, Yanina Soledad
Park, Insung
Rowe, Joan
McNabb, Bret
Ross, Pablo Juan
Belmonte, Juan Carlos Izpisua
author_sort Wu, Jun
collection PubMed
description Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.
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spelling pubmed-55852542017-09-06 CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs Wu, Jun Vilarino, Marcela Suzuki, Keiichiro Okamura, Daiji Bogliotti, Yanina Soledad Park, Insung Rowe, Joan McNabb, Bret Ross, Pablo Juan Belmonte, Juan Carlos Izpisua Sci Rep Article Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs. Nature Publishing Group UK 2017-09-05 /pmc/articles/PMC5585254/ /pubmed/28874671 http://dx.doi.org/10.1038/s41598-017-08596-5 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wu, Jun
Vilarino, Marcela
Suzuki, Keiichiro
Okamura, Daiji
Bogliotti, Yanina Soledad
Park, Insung
Rowe, Joan
McNabb, Bret
Ross, Pablo Juan
Belmonte, Juan Carlos Izpisua
CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs
title CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs
title_full CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs
title_fullStr CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs
title_full_unstemmed CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs
title_short CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs
title_sort crispr-cas9 mediated one-step disabling of pancreatogenesis in pigs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585254/
https://www.ncbi.nlm.nih.gov/pubmed/28874671
http://dx.doi.org/10.1038/s41598-017-08596-5
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