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Bone regeneration in minipigs by intrafibrillarly-mineralized collagen loaded with autologous periodontal ligament stem cells

Biomimetic intrafibrillarly-mineralized collagen (IMC) is a promising scaffold for bone regeneration because of its structural and functional similarity to natural bone. The objective of this study was to evaluate the bone regeneration potential of IMC loaded with autologous periodontal ligament ste...

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Autores principales: Zhang, Ci, Yan, Boxi, Cui, Zhen, Cui, Shengjie, Zhang, Ting, Wang, Xuedong, Liu, Dawei, Yang, Ruli, Jiang, Nan, Zhou, Yanheng, Liu, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585269/
https://www.ncbi.nlm.nih.gov/pubmed/28874877
http://dx.doi.org/10.1038/s41598-017-11155-7
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author Zhang, Ci
Yan, Boxi
Cui, Zhen
Cui, Shengjie
Zhang, Ting
Wang, Xuedong
Liu, Dawei
Yang, Ruli
Jiang, Nan
Zhou, Yanheng
Liu, Yan
author_facet Zhang, Ci
Yan, Boxi
Cui, Zhen
Cui, Shengjie
Zhang, Ting
Wang, Xuedong
Liu, Dawei
Yang, Ruli
Jiang, Nan
Zhou, Yanheng
Liu, Yan
author_sort Zhang, Ci
collection PubMed
description Biomimetic intrafibrillarly-mineralized collagen (IMC) is a promising scaffold for bone regeneration because of its structural and functional similarity to natural bone. The objective of this study was to evaluate the bone regeneration potential of IMC loaded with autologous periodontal ligament stem cells (PDLSCs) in large bone defects in minipigs. A macroporous IMC with a bone-like subfibrillar nanostructure was fabricated using a biomimetic bottom-up approach. Non-healing full thickness defects were established on the cranial bone in minipigs, and IMC and hydroxyapatite (HA) scaffolds seeded with autologous PDLSCs were implanted into these defects. Computed tomographic imaging, histology staining, and atomic force microscopy were applied to evaluate to the quantity, micro/nano structures, and mechanical performance of the neo-bone after 12 weeks of implantation. Compared with HA, IMC showed superior regeneration properties characterized by the profuse deposition of new bony structures with a normal architecture and vascularization. Immunohistochemistry showed that the runt-related transcription factor 2 and transcription factor Osterix were highly expressed in the neo-bone formed by IMC. Furthermore, the nanostructure and nanomechanics of the neo-bone formed by IMC were similar to that of natural bone. This study provides strong evidence for the future clinical applications of the IMC-based bone grafts.
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spelling pubmed-55852692017-09-06 Bone regeneration in minipigs by intrafibrillarly-mineralized collagen loaded with autologous periodontal ligament stem cells Zhang, Ci Yan, Boxi Cui, Zhen Cui, Shengjie Zhang, Ting Wang, Xuedong Liu, Dawei Yang, Ruli Jiang, Nan Zhou, Yanheng Liu, Yan Sci Rep Article Biomimetic intrafibrillarly-mineralized collagen (IMC) is a promising scaffold for bone regeneration because of its structural and functional similarity to natural bone. The objective of this study was to evaluate the bone regeneration potential of IMC loaded with autologous periodontal ligament stem cells (PDLSCs) in large bone defects in minipigs. A macroporous IMC with a bone-like subfibrillar nanostructure was fabricated using a biomimetic bottom-up approach. Non-healing full thickness defects were established on the cranial bone in minipigs, and IMC and hydroxyapatite (HA) scaffolds seeded with autologous PDLSCs were implanted into these defects. Computed tomographic imaging, histology staining, and atomic force microscopy were applied to evaluate to the quantity, micro/nano structures, and mechanical performance of the neo-bone after 12 weeks of implantation. Compared with HA, IMC showed superior regeneration properties characterized by the profuse deposition of new bony structures with a normal architecture and vascularization. Immunohistochemistry showed that the runt-related transcription factor 2 and transcription factor Osterix were highly expressed in the neo-bone formed by IMC. Furthermore, the nanostructure and nanomechanics of the neo-bone formed by IMC were similar to that of natural bone. This study provides strong evidence for the future clinical applications of the IMC-based bone grafts. Nature Publishing Group UK 2017-09-05 /pmc/articles/PMC5585269/ /pubmed/28874877 http://dx.doi.org/10.1038/s41598-017-11155-7 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhang, Ci
Yan, Boxi
Cui, Zhen
Cui, Shengjie
Zhang, Ting
Wang, Xuedong
Liu, Dawei
Yang, Ruli
Jiang, Nan
Zhou, Yanheng
Liu, Yan
Bone regeneration in minipigs by intrafibrillarly-mineralized collagen loaded with autologous periodontal ligament stem cells
title Bone regeneration in minipigs by intrafibrillarly-mineralized collagen loaded with autologous periodontal ligament stem cells
title_full Bone regeneration in minipigs by intrafibrillarly-mineralized collagen loaded with autologous periodontal ligament stem cells
title_fullStr Bone regeneration in minipigs by intrafibrillarly-mineralized collagen loaded with autologous periodontal ligament stem cells
title_full_unstemmed Bone regeneration in minipigs by intrafibrillarly-mineralized collagen loaded with autologous periodontal ligament stem cells
title_short Bone regeneration in minipigs by intrafibrillarly-mineralized collagen loaded with autologous periodontal ligament stem cells
title_sort bone regeneration in minipigs by intrafibrillarly-mineralized collagen loaded with autologous periodontal ligament stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585269/
https://www.ncbi.nlm.nih.gov/pubmed/28874877
http://dx.doi.org/10.1038/s41598-017-11155-7
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