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Synaptic connections formed by patchy projections of pyramidal cells in the superficial layers of cat visual cortex

The present study is the first to describe quantitatively the patterns of synaptic connections made by the patchy network of pyramidal cell axons in the superficial layers of cat V1 in relation to the orientation map. Intrinsic signal imaging of the orientation map was combined with 3D morphological...

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Autores principales: Koestinger, German, Martin, Kevan A. C., Roth, Stephan, Rusch, Elisha S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585309/
https://www.ncbi.nlm.nih.gov/pubmed/28243762
http://dx.doi.org/10.1007/s00429-017-1384-4
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author Koestinger, German
Martin, Kevan A. C.
Roth, Stephan
Rusch, Elisha S.
author_facet Koestinger, German
Martin, Kevan A. C.
Roth, Stephan
Rusch, Elisha S.
author_sort Koestinger, German
collection PubMed
description The present study is the first to describe quantitatively the patterns of synaptic connections made by the patchy network of pyramidal cell axons in the superficial layers of cat V1 in relation to the orientation map. Intrinsic signal imaging of the orientation map was combined with 3D morphological reconstructions of physiologically-characterized neurons at light and electron microscope levels. A Similarity Index (SI) expressed the similarity of the orientation domain of a given bouton cluster to that of its parent dendritic tree. Six pyramidal cells whose axons had a wide range of SIs were examined. Boutons were sampled from five local and five distal clusters, and from the linear segments that link the clusters. The synaptic targets were reconstructed by serial section electron microscopy. Of the 233 synapses examined, 182 synapses were formed with spiny neurons, the remainder with smooth neurons. The proportion of smooth neurons that were synaptic targets varied greatly (from 0 to 50%) between the cluster samples, but was not correlated with the SI. The postsynaptic density sizes were similar for synapses in local and distal clusters, regardless of their SI. This heterogeneity in the synaptic targets of single cells within the superficial layers is a network feature well-suited for context-dependent processing.
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spelling pubmed-55853092017-09-20 Synaptic connections formed by patchy projections of pyramidal cells in the superficial layers of cat visual cortex Koestinger, German Martin, Kevan A. C. Roth, Stephan Rusch, Elisha S. Brain Struct Funct Original Article The present study is the first to describe quantitatively the patterns of synaptic connections made by the patchy network of pyramidal cell axons in the superficial layers of cat V1 in relation to the orientation map. Intrinsic signal imaging of the orientation map was combined with 3D morphological reconstructions of physiologically-characterized neurons at light and electron microscope levels. A Similarity Index (SI) expressed the similarity of the orientation domain of a given bouton cluster to that of its parent dendritic tree. Six pyramidal cells whose axons had a wide range of SIs were examined. Boutons were sampled from five local and five distal clusters, and from the linear segments that link the clusters. The synaptic targets were reconstructed by serial section electron microscopy. Of the 233 synapses examined, 182 synapses were formed with spiny neurons, the remainder with smooth neurons. The proportion of smooth neurons that were synaptic targets varied greatly (from 0 to 50%) between the cluster samples, but was not correlated with the SI. The postsynaptic density sizes were similar for synapses in local and distal clusters, regardless of their SI. This heterogeneity in the synaptic targets of single cells within the superficial layers is a network feature well-suited for context-dependent processing. Springer Berlin Heidelberg 2017-02-27 2017 /pmc/articles/PMC5585309/ /pubmed/28243762 http://dx.doi.org/10.1007/s00429-017-1384-4 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Koestinger, German
Martin, Kevan A. C.
Roth, Stephan
Rusch, Elisha S.
Synaptic connections formed by patchy projections of pyramidal cells in the superficial layers of cat visual cortex
title Synaptic connections formed by patchy projections of pyramidal cells in the superficial layers of cat visual cortex
title_full Synaptic connections formed by patchy projections of pyramidal cells in the superficial layers of cat visual cortex
title_fullStr Synaptic connections formed by patchy projections of pyramidal cells in the superficial layers of cat visual cortex
title_full_unstemmed Synaptic connections formed by patchy projections of pyramidal cells in the superficial layers of cat visual cortex
title_short Synaptic connections formed by patchy projections of pyramidal cells in the superficial layers of cat visual cortex
title_sort synaptic connections formed by patchy projections of pyramidal cells in the superficial layers of cat visual cortex
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585309/
https://www.ncbi.nlm.nih.gov/pubmed/28243762
http://dx.doi.org/10.1007/s00429-017-1384-4
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