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Tumor cells and their crosstalk with endothelial cells in 3D spheroids
Recapitulating the tumor microenvironment is a central challenge in the development of experimental model for cancer. To provide a reliable tool for drug development and for personalized cancer therapy, it is critical to maintain key features that exist in the original tumor. Along with this effort...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585367/ https://www.ncbi.nlm.nih.gov/pubmed/28874803 http://dx.doi.org/10.1038/s41598-017-10699-y |
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author | Shoval, Hila Karsch-Bluman, Adi Brill-Karniely, Yifat Stern, Tal Zamir, Gideon Hubert, Ayala Benny, Ofra |
author_facet | Shoval, Hila Karsch-Bluman, Adi Brill-Karniely, Yifat Stern, Tal Zamir, Gideon Hubert, Ayala Benny, Ofra |
author_sort | Shoval, Hila |
collection | PubMed |
description | Recapitulating the tumor microenvironment is a central challenge in the development of experimental model for cancer. To provide a reliable tool for drug development and for personalized cancer therapy, it is critical to maintain key features that exist in the original tumor. Along with this effort, 3-dimentional (3D) cellular models are being extensively studied. Spheroids are self-assembled cell aggregates that possess many important components of the physiological spatial growth and cell-cell interactions. In this study we aimed to investigate the interconnection between tumor and endothelial cells (EC) in hybrid spheroids containing either tumor cell (TC) lines or patient derived cancer cells. Preparation protocols of hybrid spheroids were optimized and their morphology and tissue-like features were analyzed. Our finding show that capillary-like structures are formed upon assembly and growth of TC:EC spheroids and that spheroids’ shape and surface texture may be an indication of spatial invasiveness of cells in the extra-cellular matrix (ECM). Establishing a model of hybrid tumor/stroma spheroids has a crucial importance in the experimental approach for personalized medicine, and may offer a reliable and low-cost method for the goal of predicting drug effects. |
format | Online Article Text |
id | pubmed-5585367 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55853672017-09-06 Tumor cells and their crosstalk with endothelial cells in 3D spheroids Shoval, Hila Karsch-Bluman, Adi Brill-Karniely, Yifat Stern, Tal Zamir, Gideon Hubert, Ayala Benny, Ofra Sci Rep Article Recapitulating the tumor microenvironment is a central challenge in the development of experimental model for cancer. To provide a reliable tool for drug development and for personalized cancer therapy, it is critical to maintain key features that exist in the original tumor. Along with this effort, 3-dimentional (3D) cellular models are being extensively studied. Spheroids are self-assembled cell aggregates that possess many important components of the physiological spatial growth and cell-cell interactions. In this study we aimed to investigate the interconnection between tumor and endothelial cells (EC) in hybrid spheroids containing either tumor cell (TC) lines or patient derived cancer cells. Preparation protocols of hybrid spheroids were optimized and their morphology and tissue-like features were analyzed. Our finding show that capillary-like structures are formed upon assembly and growth of TC:EC spheroids and that spheroids’ shape and surface texture may be an indication of spatial invasiveness of cells in the extra-cellular matrix (ECM). Establishing a model of hybrid tumor/stroma spheroids has a crucial importance in the experimental approach for personalized medicine, and may offer a reliable and low-cost method for the goal of predicting drug effects. Nature Publishing Group UK 2017-09-05 /pmc/articles/PMC5585367/ /pubmed/28874803 http://dx.doi.org/10.1038/s41598-017-10699-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Shoval, Hila Karsch-Bluman, Adi Brill-Karniely, Yifat Stern, Tal Zamir, Gideon Hubert, Ayala Benny, Ofra Tumor cells and their crosstalk with endothelial cells in 3D spheroids |
title | Tumor cells and their crosstalk with endothelial cells in 3D spheroids |
title_full | Tumor cells and their crosstalk with endothelial cells in 3D spheroids |
title_fullStr | Tumor cells and their crosstalk with endothelial cells in 3D spheroids |
title_full_unstemmed | Tumor cells and their crosstalk with endothelial cells in 3D spheroids |
title_short | Tumor cells and their crosstalk with endothelial cells in 3D spheroids |
title_sort | tumor cells and their crosstalk with endothelial cells in 3d spheroids |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585367/ https://www.ncbi.nlm.nih.gov/pubmed/28874803 http://dx.doi.org/10.1038/s41598-017-10699-y |
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