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Kinetics of Circulating MicroRNAs in Response to Cardiac Stress in Patients With Coronary Artery Disease
BACKGROUND: Circulating microRNAs (miRNAs/miRs) are regulated in patients with coronary artery disease. The impact of transient coronary ischemia on circulating miRNA levels is unknown. We aimed to investigate circulating miRNA kinetics in response to cardiac stress in patients with or without signi...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5586407/ https://www.ncbi.nlm.nih.gov/pubmed/28751542 http://dx.doi.org/10.1161/JAHA.116.005270 |
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author | Jansen, Felix Schäfer, Lisa Wang, Han Schmitz, Theresa Flender, Anna Schueler, Robert Hammerstingl, Christoph Nickenig, Georg Sinning, Jan‐Malte Werner, Nikos |
author_facet | Jansen, Felix Schäfer, Lisa Wang, Han Schmitz, Theresa Flender, Anna Schueler, Robert Hammerstingl, Christoph Nickenig, Georg Sinning, Jan‐Malte Werner, Nikos |
author_sort | Jansen, Felix |
collection | PubMed |
description | BACKGROUND: Circulating microRNAs (miRNAs/miRs) are regulated in patients with coronary artery disease. The impact of transient coronary ischemia on circulating miRNA levels is unknown. We aimed to investigate circulating miRNA kinetics in response to cardiac stress in patients with or without significant coronary stenosis. METHODS AND RESULTS: Eighty of 105 screened patients with stable coronary artery disease underwent dobutamine stress echocardiography before coronary angiography. Nine circulating vascular miRNAs (miRNA‐21, miRNA‐26, miRNA‐27a, miRNA‐92a, miRNA‐126‐3p, miRNA‐133a, miRNA‐222, miRNA‐223, and miRNA‐199‐5p) were quantified in plasma by reverse transcription polymerase chain reaction before, immediately after, and 4 and 24 hours after dobutamine stress echocardiography. Quantitative polymerase chain reaction revealed increased miRNA‐21, miRNA‐126‐3p, and miRNA‐222 levels at 24 hours after dobutamine stress echocardiography in all patients. On coronary angiography, significant coronary artery stenoses (>80% diameter stenosis) were found in 41 patients. Stratifying patients according to the prevalence of significant stenoses, patients with stenosis showed an increase of circulating miRNA‐21, miRNA‐126‐3p, and miRNA‐222 in response to cardiac stress. In patients without significant stenoses (<50% diameter stenosis), miRNA‐92a levels gradually increased in response to cardiac stress. CONCLUSIONS: miRNAs are distinctly released into the circulation in response to cardiac stress depending on the prevalence of significant coronary stenoses. |
format | Online Article Text |
id | pubmed-5586407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-55864072017-09-11 Kinetics of Circulating MicroRNAs in Response to Cardiac Stress in Patients With Coronary Artery Disease Jansen, Felix Schäfer, Lisa Wang, Han Schmitz, Theresa Flender, Anna Schueler, Robert Hammerstingl, Christoph Nickenig, Georg Sinning, Jan‐Malte Werner, Nikos J Am Heart Assoc Original Research BACKGROUND: Circulating microRNAs (miRNAs/miRs) are regulated in patients with coronary artery disease. The impact of transient coronary ischemia on circulating miRNA levels is unknown. We aimed to investigate circulating miRNA kinetics in response to cardiac stress in patients with or without significant coronary stenosis. METHODS AND RESULTS: Eighty of 105 screened patients with stable coronary artery disease underwent dobutamine stress echocardiography before coronary angiography. Nine circulating vascular miRNAs (miRNA‐21, miRNA‐26, miRNA‐27a, miRNA‐92a, miRNA‐126‐3p, miRNA‐133a, miRNA‐222, miRNA‐223, and miRNA‐199‐5p) were quantified in plasma by reverse transcription polymerase chain reaction before, immediately after, and 4 and 24 hours after dobutamine stress echocardiography. Quantitative polymerase chain reaction revealed increased miRNA‐21, miRNA‐126‐3p, and miRNA‐222 levels at 24 hours after dobutamine stress echocardiography in all patients. On coronary angiography, significant coronary artery stenoses (>80% diameter stenosis) were found in 41 patients. Stratifying patients according to the prevalence of significant stenoses, patients with stenosis showed an increase of circulating miRNA‐21, miRNA‐126‐3p, and miRNA‐222 in response to cardiac stress. In patients without significant stenoses (<50% diameter stenosis), miRNA‐92a levels gradually increased in response to cardiac stress. CONCLUSIONS: miRNAs are distinctly released into the circulation in response to cardiac stress depending on the prevalence of significant coronary stenoses. John Wiley and Sons Inc. 2017-07-27 /pmc/articles/PMC5586407/ /pubmed/28751542 http://dx.doi.org/10.1161/JAHA.116.005270 Text en © 2017 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Jansen, Felix Schäfer, Lisa Wang, Han Schmitz, Theresa Flender, Anna Schueler, Robert Hammerstingl, Christoph Nickenig, Georg Sinning, Jan‐Malte Werner, Nikos Kinetics of Circulating MicroRNAs in Response to Cardiac Stress in Patients With Coronary Artery Disease |
title | Kinetics of Circulating MicroRNAs in Response to Cardiac Stress in Patients With Coronary Artery Disease |
title_full | Kinetics of Circulating MicroRNAs in Response to Cardiac Stress in Patients With Coronary Artery Disease |
title_fullStr | Kinetics of Circulating MicroRNAs in Response to Cardiac Stress in Patients With Coronary Artery Disease |
title_full_unstemmed | Kinetics of Circulating MicroRNAs in Response to Cardiac Stress in Patients With Coronary Artery Disease |
title_short | Kinetics of Circulating MicroRNAs in Response to Cardiac Stress in Patients With Coronary Artery Disease |
title_sort | kinetics of circulating micrornas in response to cardiac stress in patients with coronary artery disease |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5586407/ https://www.ncbi.nlm.nih.gov/pubmed/28751542 http://dx.doi.org/10.1161/JAHA.116.005270 |
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