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Genotypes and Virulence Genes in Group B Streptococcus Isolated in the Maternity Hospital, Kuwait

OBJECTIVE: To characterize group B streptococcus (GBS) isolates obtained from patients at the Maternity Hospital in Kuwait for their genotypes and carriage of virulence genes. MATERIALS AND METHODS: A total of 154 GBS isolates were obtained from July 1 to October 31, 2007, from vaginal swabs (n = 95...

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Autores principales: Udo, Edet E., Boswihi, Samar S., Al-Sweih, Noura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: S. Karger AG 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5586775/
https://www.ncbi.nlm.nih.gov/pubmed/23571853
http://dx.doi.org/10.1159/000349932
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author Udo, Edet E.
Boswihi, Samar S.
Al-Sweih, Noura
author_facet Udo, Edet E.
Boswihi, Samar S.
Al-Sweih, Noura
author_sort Udo, Edet E.
collection PubMed
description OBJECTIVE: To characterize group B streptococcus (GBS) isolates obtained from patients at the Maternity Hospital in Kuwait for their genotypes and carriage of virulence genes. MATERIALS AND METHODS: A total of 154 GBS isolates were obtained from July 1 to October 31, 2007, from vaginal swabs (n = 95), urine (n = 46), blood (n = 4) and miscellaneous sources (n = 9). Genotypes were obtained by pulsed-field gel electrophoresis (PFGE), following digestion with SmaI or EagI restriction enzymes. PCR was used to screen for the carriage of virulence genes including: surface protein of group B streptococcus (spb1), secreted fibrinogen-binding protein (fbsB), C5a peptidase (scpB), laminin-binding protein (lmb), α− (bca) and β-subunits of the C protein (bac), resistance to protease immunity protein (rib), and phage-associated gene (pag); regulatory protein (dltR), and toxins CAMP factor (cfb), hyaluronidase (hylB) and superoxide dismutase (sodA). RESULTS: PFGE defined 14 genotypes differentiating isolates with the same serotypes into different genetic backgrounds. All isolates contained genes for virulence factors. However, cfb (99.4%), scpB (88.3%), lmb (88.3%), bca (57.8%), sodA (55.8%) and dltR (53.9%) were the common virulence genes. In total, 144 (90.3%) of the isolates contained 3 or more virulence genes. However, while cfb, lmb and scpB occurred in all genotypes, others occurred in some but not in all genotypes. CONCLUSIONS: GBS isolates obtained at the Maternity Hospital, Kuwait, belonged to diverse genetic backgrounds with the majority carrying multiple virulence genes.
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spelling pubmed-55867752017-11-01 Genotypes and Virulence Genes in Group B Streptococcus Isolated in the Maternity Hospital, Kuwait Udo, Edet E. Boswihi, Samar S. Al-Sweih, Noura Med Princ Pract Original Paper OBJECTIVE: To characterize group B streptococcus (GBS) isolates obtained from patients at the Maternity Hospital in Kuwait for their genotypes and carriage of virulence genes. MATERIALS AND METHODS: A total of 154 GBS isolates were obtained from July 1 to October 31, 2007, from vaginal swabs (n = 95), urine (n = 46), blood (n = 4) and miscellaneous sources (n = 9). Genotypes were obtained by pulsed-field gel electrophoresis (PFGE), following digestion with SmaI or EagI restriction enzymes. PCR was used to screen for the carriage of virulence genes including: surface protein of group B streptococcus (spb1), secreted fibrinogen-binding protein (fbsB), C5a peptidase (scpB), laminin-binding protein (lmb), α− (bca) and β-subunits of the C protein (bac), resistance to protease immunity protein (rib), and phage-associated gene (pag); regulatory protein (dltR), and toxins CAMP factor (cfb), hyaluronidase (hylB) and superoxide dismutase (sodA). RESULTS: PFGE defined 14 genotypes differentiating isolates with the same serotypes into different genetic backgrounds. All isolates contained genes for virulence factors. However, cfb (99.4%), scpB (88.3%), lmb (88.3%), bca (57.8%), sodA (55.8%) and dltR (53.9%) were the common virulence genes. In total, 144 (90.3%) of the isolates contained 3 or more virulence genes. However, while cfb, lmb and scpB occurred in all genotypes, others occurred in some but not in all genotypes. CONCLUSIONS: GBS isolates obtained at the Maternity Hospital, Kuwait, belonged to diverse genetic backgrounds with the majority carrying multiple virulence genes. S. Karger AG 2013-09 2013-04-05 /pmc/articles/PMC5586775/ /pubmed/23571853 http://dx.doi.org/10.1159/000349932 Text en Copyright © 2013 by S. Karger AG, Basel http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article licensed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported license (CC BY-NC) (www.karger.com/OA-license), applicable to the online version of the article only. Distribution permitted for non-commercial purposes only.
spellingShingle Original Paper
Udo, Edet E.
Boswihi, Samar S.
Al-Sweih, Noura
Genotypes and Virulence Genes in Group B Streptococcus Isolated in the Maternity Hospital, Kuwait
title Genotypes and Virulence Genes in Group B Streptococcus Isolated in the Maternity Hospital, Kuwait
title_full Genotypes and Virulence Genes in Group B Streptococcus Isolated in the Maternity Hospital, Kuwait
title_fullStr Genotypes and Virulence Genes in Group B Streptococcus Isolated in the Maternity Hospital, Kuwait
title_full_unstemmed Genotypes and Virulence Genes in Group B Streptococcus Isolated in the Maternity Hospital, Kuwait
title_short Genotypes and Virulence Genes in Group B Streptococcus Isolated in the Maternity Hospital, Kuwait
title_sort genotypes and virulence genes in group b streptococcus isolated in the maternity hospital, kuwait
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5586775/
https://www.ncbi.nlm.nih.gov/pubmed/23571853
http://dx.doi.org/10.1159/000349932
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