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DNA aneuploidy relationship with patient age and tobacco smoke in OPMDs/OSCCs
The aim of this study was to investigate the relationship between tobacco smoke habit, patient age, DNA aneuploidy and genomic DNA copy number aberrations (CNAs) in oral potentially malignant disorder (OPMD) and oral squamous cell carcinoma (OSCC) patients. DNA aneuploidy was detected by high-resolu...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587305/ https://www.ncbi.nlm.nih.gov/pubmed/28877236 http://dx.doi.org/10.1371/journal.pone.0184425 |
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author | Castagnola, Patrizio Gandolfo, Sergio Malacarne, Davide Aiello, Cinzia Marino, Roberto Zoppoli, Gabriele Ballestrero, Alberto Giaretti, Walter Pentenero, Monica |
author_facet | Castagnola, Patrizio Gandolfo, Sergio Malacarne, Davide Aiello, Cinzia Marino, Roberto Zoppoli, Gabriele Ballestrero, Alberto Giaretti, Walter Pentenero, Monica |
author_sort | Castagnola, Patrizio |
collection | PubMed |
description | The aim of this study was to investigate the relationship between tobacco smoke habit, patient age, DNA aneuploidy and genomic DNA copy number aberrations (CNAs) in oral potentially malignant disorder (OPMD) and oral squamous cell carcinoma (OSCC) patients. DNA aneuploidy was detected by high-resolution DNA flow cytometry (hr DNA-FCM) on DAPI stained nuclei obtained from multiple tissue samples from OPMDs/OSCCs in 220 consecutive patients. Nuclear genomic aberrations were determined in a subset of 65 patients by genome-wide array comparative genomic hybridization (aCGH) using DNA extracted from either diploid or aneuploid nuclei suspension sorted by FCM. DNA aneuploidy and mean nuclear genomic aberrations were associated with patients’ age. In particular, DNA aneuploidy strongly associated with age in non-smoker OPMDs/OSCCs patients. OSCCs from smokers showed a lower prevalence of DNA aneuploidy compared to OSCCs from non-smokers. A higher occurrence of DNA aneuploidy (particularly in smokers’ OPMDs) was observed in patients characterized by involvement of a single oral subsite. Our study suggests that: 1) DNA aneuploidy in non-smokers is mainly related to aging; 2) OPMDs/OSCCs involving multiple oral subsites in smokers are less likely to develop DNA aneuploidy compared to non-smokers; 3) OSCC development is characterized by both CIN and CIN-independent mechanisms and that the latter are more relevant in smokers. This study provides evidence that DNA diploid OPMDs may be considered at lower risk of cancerization than DNA aneuploid ones in non-smokers but not in smokers. |
format | Online Article Text |
id | pubmed-5587305 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-55873052017-09-15 DNA aneuploidy relationship with patient age and tobacco smoke in OPMDs/OSCCs Castagnola, Patrizio Gandolfo, Sergio Malacarne, Davide Aiello, Cinzia Marino, Roberto Zoppoli, Gabriele Ballestrero, Alberto Giaretti, Walter Pentenero, Monica PLoS One Research Article The aim of this study was to investigate the relationship between tobacco smoke habit, patient age, DNA aneuploidy and genomic DNA copy number aberrations (CNAs) in oral potentially malignant disorder (OPMD) and oral squamous cell carcinoma (OSCC) patients. DNA aneuploidy was detected by high-resolution DNA flow cytometry (hr DNA-FCM) on DAPI stained nuclei obtained from multiple tissue samples from OPMDs/OSCCs in 220 consecutive patients. Nuclear genomic aberrations were determined in a subset of 65 patients by genome-wide array comparative genomic hybridization (aCGH) using DNA extracted from either diploid or aneuploid nuclei suspension sorted by FCM. DNA aneuploidy and mean nuclear genomic aberrations were associated with patients’ age. In particular, DNA aneuploidy strongly associated with age in non-smoker OPMDs/OSCCs patients. OSCCs from smokers showed a lower prevalence of DNA aneuploidy compared to OSCCs from non-smokers. A higher occurrence of DNA aneuploidy (particularly in smokers’ OPMDs) was observed in patients characterized by involvement of a single oral subsite. Our study suggests that: 1) DNA aneuploidy in non-smokers is mainly related to aging; 2) OPMDs/OSCCs involving multiple oral subsites in smokers are less likely to develop DNA aneuploidy compared to non-smokers; 3) OSCC development is characterized by both CIN and CIN-independent mechanisms and that the latter are more relevant in smokers. This study provides evidence that DNA diploid OPMDs may be considered at lower risk of cancerization than DNA aneuploid ones in non-smokers but not in smokers. Public Library of Science 2017-09-06 /pmc/articles/PMC5587305/ /pubmed/28877236 http://dx.doi.org/10.1371/journal.pone.0184425 Text en © 2017 Castagnola et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Castagnola, Patrizio Gandolfo, Sergio Malacarne, Davide Aiello, Cinzia Marino, Roberto Zoppoli, Gabriele Ballestrero, Alberto Giaretti, Walter Pentenero, Monica DNA aneuploidy relationship with patient age and tobacco smoke in OPMDs/OSCCs |
title | DNA aneuploidy relationship with patient age and tobacco smoke in OPMDs/OSCCs |
title_full | DNA aneuploidy relationship with patient age and tobacco smoke in OPMDs/OSCCs |
title_fullStr | DNA aneuploidy relationship with patient age and tobacco smoke in OPMDs/OSCCs |
title_full_unstemmed | DNA aneuploidy relationship with patient age and tobacco smoke in OPMDs/OSCCs |
title_short | DNA aneuploidy relationship with patient age and tobacco smoke in OPMDs/OSCCs |
title_sort | dna aneuploidy relationship with patient age and tobacco smoke in opmds/osccs |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587305/ https://www.ncbi.nlm.nih.gov/pubmed/28877236 http://dx.doi.org/10.1371/journal.pone.0184425 |
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