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Proteomic analysis on roots of Oenothera glazioviana under copper-stress conditions

Proteomic studies were performed to identify proteins involved in the response of Oenothera glazioviana seedlings under Cu stress. Exposure of 28-d-old seedlings to 50 μM CuSO4 for 3 d led to inhibition of shoot and root growth as well as a considerable increase in the level of lipid peroxidation in...

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Autores principales: Wang, Chong, Wang, Jie, Wang, Xiao, Xia, Yan, Chen, Chen, Shen, Zhenguo, Chen, Yahua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587583/
https://www.ncbi.nlm.nih.gov/pubmed/28878286
http://dx.doi.org/10.1038/s41598-017-10370-6
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author Wang, Chong
Wang, Jie
Wang, Xiao
Xia, Yan
Chen, Chen
Shen, Zhenguo
Chen, Yahua
author_facet Wang, Chong
Wang, Jie
Wang, Xiao
Xia, Yan
Chen, Chen
Shen, Zhenguo
Chen, Yahua
author_sort Wang, Chong
collection PubMed
description Proteomic studies were performed to identify proteins involved in the response of Oenothera glazioviana seedlings under Cu stress. Exposure of 28-d-old seedlings to 50 μM CuSO4 for 3 d led to inhibition of shoot and root growth as well as a considerable increase in the level of lipid peroxidation in the roots. Cu absorbed by O. glazioviana accumulated more easily in the root than in the shoot. Label-free proteomic analysis indicated 58 differentially abundant proteins (DAPs) of the total 3,149 proteins in the roots of O. glazioviana seedlings, of which 36 were upregulated and 22 were downregulated under Cu stress conditions. Gene Ontology analysis showed that most of the identified proteins could be annotated to signal transduction, detoxification, stress defence, carbohydrate, energy, and protein metabolism, development, and oxidoreduction. We also retrieved 13 proteins from the enriched Kyoto Encyclopaedia of Genes and Genomes and the protein-protein interaction databases related to various pathways, including the citric acid (CA) cycle. Application of exogenous CA to O. glazioviana seedlings exposed to Cu alleviated the stress symptoms. Overall, this study provided new insights into the molecular mechanisms of plant response to Cu at the protein level in relation to soil properties.
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spelling pubmed-55875832017-09-13 Proteomic analysis on roots of Oenothera glazioviana under copper-stress conditions Wang, Chong Wang, Jie Wang, Xiao Xia, Yan Chen, Chen Shen, Zhenguo Chen, Yahua Sci Rep Article Proteomic studies were performed to identify proteins involved in the response of Oenothera glazioviana seedlings under Cu stress. Exposure of 28-d-old seedlings to 50 μM CuSO4 for 3 d led to inhibition of shoot and root growth as well as a considerable increase in the level of lipid peroxidation in the roots. Cu absorbed by O. glazioviana accumulated more easily in the root than in the shoot. Label-free proteomic analysis indicated 58 differentially abundant proteins (DAPs) of the total 3,149 proteins in the roots of O. glazioviana seedlings, of which 36 were upregulated and 22 were downregulated under Cu stress conditions. Gene Ontology analysis showed that most of the identified proteins could be annotated to signal transduction, detoxification, stress defence, carbohydrate, energy, and protein metabolism, development, and oxidoreduction. We also retrieved 13 proteins from the enriched Kyoto Encyclopaedia of Genes and Genomes and the protein-protein interaction databases related to various pathways, including the citric acid (CA) cycle. Application of exogenous CA to O. glazioviana seedlings exposed to Cu alleviated the stress symptoms. Overall, this study provided new insights into the molecular mechanisms of plant response to Cu at the protein level in relation to soil properties. Nature Publishing Group UK 2017-09-06 /pmc/articles/PMC5587583/ /pubmed/28878286 http://dx.doi.org/10.1038/s41598-017-10370-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wang, Chong
Wang, Jie
Wang, Xiao
Xia, Yan
Chen, Chen
Shen, Zhenguo
Chen, Yahua
Proteomic analysis on roots of Oenothera glazioviana under copper-stress conditions
title Proteomic analysis on roots of Oenothera glazioviana under copper-stress conditions
title_full Proteomic analysis on roots of Oenothera glazioviana under copper-stress conditions
title_fullStr Proteomic analysis on roots of Oenothera glazioviana under copper-stress conditions
title_full_unstemmed Proteomic analysis on roots of Oenothera glazioviana under copper-stress conditions
title_short Proteomic analysis on roots of Oenothera glazioviana under copper-stress conditions
title_sort proteomic analysis on roots of oenothera glazioviana under copper-stress conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587583/
https://www.ncbi.nlm.nih.gov/pubmed/28878286
http://dx.doi.org/10.1038/s41598-017-10370-6
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