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A Method for Combined Retinal Vascular and Tissue Oxygen Tension Imaging

The retina requires adequate oxygenation to maintain cellular metabolism and visual function. Inner retinal oxygen metabolism is directly related to retinal vascular oxygen tension (PO(2)) and inner retinal oxygen extraction fraction (OEF), whereas outer retinal oxygen consumption (QO(2)) relies on...

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Autores principales: Felder, Anthony E., Wanek, Justin, Tan, Michael R., Blair, Norman P., Shahidi, Mahnaz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587610/
https://www.ncbi.nlm.nih.gov/pubmed/28878307
http://dx.doi.org/10.1038/s41598-017-10955-1
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author Felder, Anthony E.
Wanek, Justin
Tan, Michael R.
Blair, Norman P.
Shahidi, Mahnaz
author_facet Felder, Anthony E.
Wanek, Justin
Tan, Michael R.
Blair, Norman P.
Shahidi, Mahnaz
author_sort Felder, Anthony E.
collection PubMed
description The retina requires adequate oxygenation to maintain cellular metabolism and visual function. Inner retinal oxygen metabolism is directly related to retinal vascular oxygen tension (PO(2)) and inner retinal oxygen extraction fraction (OEF), whereas outer retinal oxygen consumption (QO(2)) relies on oxygen availability by the choroid and is contingent upon retinal tissue oxygen tension (tPO(2)) gradients across the retinal depth. Thus far, these oxygenation and metabolic parameters have been measured independently by different techniques in separate animals, precluding a comprehensive and correlative assessment of retinal oxygenation and metabolism dynamics. The purpose of the current study is to report an innovative optical system for dual oxyphor phosphorescence lifetime imaging to near-simultaneously measure retinal vascular PO(2) and tPO(2) in rats. The use of a new oxyphor with different spectral characteristics allowed differentiation of phosphorescence signals from the retinal vasculature and tissue. Concurrent measurements of retinal arterial and venous PO(2), tPO(2) through the retinal depth, inner retinal OEF, and outer retinal QO(2) were demonstrated, permitting a correlative assessment of retinal oxygenation and metabolism. Future application of this method can be used to investigate the relations among retinal oxygen content, extraction and metabolism under pathologic conditions and thus advance knowledge of retinal hypoxia pathophysiology.
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spelling pubmed-55876102017-09-13 A Method for Combined Retinal Vascular and Tissue Oxygen Tension Imaging Felder, Anthony E. Wanek, Justin Tan, Michael R. Blair, Norman P. Shahidi, Mahnaz Sci Rep Article The retina requires adequate oxygenation to maintain cellular metabolism and visual function. Inner retinal oxygen metabolism is directly related to retinal vascular oxygen tension (PO(2)) and inner retinal oxygen extraction fraction (OEF), whereas outer retinal oxygen consumption (QO(2)) relies on oxygen availability by the choroid and is contingent upon retinal tissue oxygen tension (tPO(2)) gradients across the retinal depth. Thus far, these oxygenation and metabolic parameters have been measured independently by different techniques in separate animals, precluding a comprehensive and correlative assessment of retinal oxygenation and metabolism dynamics. The purpose of the current study is to report an innovative optical system for dual oxyphor phosphorescence lifetime imaging to near-simultaneously measure retinal vascular PO(2) and tPO(2) in rats. The use of a new oxyphor with different spectral characteristics allowed differentiation of phosphorescence signals from the retinal vasculature and tissue. Concurrent measurements of retinal arterial and venous PO(2), tPO(2) through the retinal depth, inner retinal OEF, and outer retinal QO(2) were demonstrated, permitting a correlative assessment of retinal oxygenation and metabolism. Future application of this method can be used to investigate the relations among retinal oxygen content, extraction and metabolism under pathologic conditions and thus advance knowledge of retinal hypoxia pathophysiology. Nature Publishing Group UK 2017-09-06 /pmc/articles/PMC5587610/ /pubmed/28878307 http://dx.doi.org/10.1038/s41598-017-10955-1 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Felder, Anthony E.
Wanek, Justin
Tan, Michael R.
Blair, Norman P.
Shahidi, Mahnaz
A Method for Combined Retinal Vascular and Tissue Oxygen Tension Imaging
title A Method for Combined Retinal Vascular and Tissue Oxygen Tension Imaging
title_full A Method for Combined Retinal Vascular and Tissue Oxygen Tension Imaging
title_fullStr A Method for Combined Retinal Vascular and Tissue Oxygen Tension Imaging
title_full_unstemmed A Method for Combined Retinal Vascular and Tissue Oxygen Tension Imaging
title_short A Method for Combined Retinal Vascular and Tissue Oxygen Tension Imaging
title_sort method for combined retinal vascular and tissue oxygen tension imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587610/
https://www.ncbi.nlm.nih.gov/pubmed/28878307
http://dx.doi.org/10.1038/s41598-017-10955-1
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