In-line balanced detection stimulated Raman scattering microscopy

We introduce a novel configuration for stimulated Raman scattering (SRS) microscopy, called In-line Balanced Detection (IBD), which employs a birefringent plate to generate a time-delayed polarization-multiplexed collinear replica of the probe, acting as a reference. Probe and reference cross the sa...

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Autores principales: Crisafi, Francesco, Kumar, Vikas, Scopigno, Tullio, Marangoni, Marco, Cerullo, Giulio, Polli, Dario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587718/
https://www.ncbi.nlm.nih.gov/pubmed/28878228
http://dx.doi.org/10.1038/s41598-017-09839-1
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author Crisafi, Francesco
Kumar, Vikas
Scopigno, Tullio
Marangoni, Marco
Cerullo, Giulio
Polli, Dario
author_facet Crisafi, Francesco
Kumar, Vikas
Scopigno, Tullio
Marangoni, Marco
Cerullo, Giulio
Polli, Dario
author_sort Crisafi, Francesco
collection PubMed
description We introduce a novel configuration for stimulated Raman scattering (SRS) microscopy, called In-line Balanced Detection (IBD), which employs a birefringent plate to generate a time-delayed polarization-multiplexed collinear replica of the probe, acting as a reference. Probe and reference cross the sample at the same position, thus maintaining their balance during image acquisition. IBD can be implemented in any conventional SRS setup, by adding a few simple elements, bringing its sensitivity close to the shot-noise limit even with a noisy laser. We tested IBD with a fiber-format laser system and observed signal-to-noise ratio improvement by up to 30 dB.
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spelling pubmed-55877182017-09-13 In-line balanced detection stimulated Raman scattering microscopy Crisafi, Francesco Kumar, Vikas Scopigno, Tullio Marangoni, Marco Cerullo, Giulio Polli, Dario Sci Rep Article We introduce a novel configuration for stimulated Raman scattering (SRS) microscopy, called In-line Balanced Detection (IBD), which employs a birefringent plate to generate a time-delayed polarization-multiplexed collinear replica of the probe, acting as a reference. Probe and reference cross the sample at the same position, thus maintaining their balance during image acquisition. IBD can be implemented in any conventional SRS setup, by adding a few simple elements, bringing its sensitivity close to the shot-noise limit even with a noisy laser. We tested IBD with a fiber-format laser system and observed signal-to-noise ratio improvement by up to 30 dB. Nature Publishing Group UK 2017-09-06 /pmc/articles/PMC5587718/ /pubmed/28878228 http://dx.doi.org/10.1038/s41598-017-09839-1 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Crisafi, Francesco
Kumar, Vikas
Scopigno, Tullio
Marangoni, Marco
Cerullo, Giulio
Polli, Dario
In-line balanced detection stimulated Raman scattering microscopy
title In-line balanced detection stimulated Raman scattering microscopy
title_full In-line balanced detection stimulated Raman scattering microscopy
title_fullStr In-line balanced detection stimulated Raman scattering microscopy
title_full_unstemmed In-line balanced detection stimulated Raman scattering microscopy
title_short In-line balanced detection stimulated Raman scattering microscopy
title_sort in-line balanced detection stimulated raman scattering microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587718/
https://www.ncbi.nlm.nih.gov/pubmed/28878228
http://dx.doi.org/10.1038/s41598-017-09839-1
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