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Dietary iron restriction alleviates renal tubulointerstitial injury induced by protein overload in mice
Increased proteinuria causes tubulointerstitial injury due to inflammation in chronic kidney disease (CKD). Iron restriction exhibits protective effects against renal dysfunction; however, its effects against protein overload-induced tubulointerstitial damage remain unclear. Here, we investigated di...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587788/ https://www.ncbi.nlm.nih.gov/pubmed/28878231 http://dx.doi.org/10.1038/s41598-017-11089-0 |
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author | Ikeda, Yasumasa Horinouchi, Yuya Hamano, Hirofumi Hirayama, Tasuku Kishi, Seiji Izawa-Ishizawa, Yuki Imanishi, Masaki Zamami, Yoshito Takechi, Kenshi Miyamoto, Licht Ishizawa, Keisuke Aihara, Ken-ichi Nagasawa, Hideko Tsuchiya, Koichiro Tamaki, Toshiaki |
author_facet | Ikeda, Yasumasa Horinouchi, Yuya Hamano, Hirofumi Hirayama, Tasuku Kishi, Seiji Izawa-Ishizawa, Yuki Imanishi, Masaki Zamami, Yoshito Takechi, Kenshi Miyamoto, Licht Ishizawa, Keisuke Aihara, Ken-ichi Nagasawa, Hideko Tsuchiya, Koichiro Tamaki, Toshiaki |
author_sort | Ikeda, Yasumasa |
collection | PubMed |
description | Increased proteinuria causes tubulointerstitial injury due to inflammation in chronic kidney disease (CKD). Iron restriction exhibits protective effects against renal dysfunction; however, its effects against protein overload-induced tubulointerstitial damage remain unclear. Here, we investigated dietary iron restriction effect on tubulointerstitial damage in mice with protein-overload tubulointerstitial injury. Renal tubulointerstitial injury in animal model was induced by intraperitoneal injection of an overdose of bovine serum albumin (BSA). We divided mice into three groups: normal saline + normal diet (ND), BSA + ND, and BSA + iron-restricted diet (IRD). BSA overload induced renal tubulointerstitial injury in the ND mice, which was ameliorated in the IRD mice. Inflammatory cytokines and extracellular matrix mRNA expression was upregulated in BSA + ND mice kidneys and was inhibited by IRD. BSA-induced increase in renal superoxide production, NADPH oxidase activity, and p22(phox) expression was diminished in the IRD mice. IRD suppression increased BSA-induced renal macrophage infiltration. Moreover, BSA mice exhibited nucleotide-binding oligomerisation domain-like receptor pyrin domain-containing protein (NLRP) inflammasome activation, which was inhibited by IRD. Ferrous iron increased in kidneys with BSA overload and was inhibited by IRD. Thus, iron restriction inhibited oxidative stress and inflammatory changes, contributing to the protective effect against BSA overload-induced tubulointerstitial injury. |
format | Online Article Text |
id | pubmed-5587788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55877882017-09-13 Dietary iron restriction alleviates renal tubulointerstitial injury induced by protein overload in mice Ikeda, Yasumasa Horinouchi, Yuya Hamano, Hirofumi Hirayama, Tasuku Kishi, Seiji Izawa-Ishizawa, Yuki Imanishi, Masaki Zamami, Yoshito Takechi, Kenshi Miyamoto, Licht Ishizawa, Keisuke Aihara, Ken-ichi Nagasawa, Hideko Tsuchiya, Koichiro Tamaki, Toshiaki Sci Rep Article Increased proteinuria causes tubulointerstitial injury due to inflammation in chronic kidney disease (CKD). Iron restriction exhibits protective effects against renal dysfunction; however, its effects against protein overload-induced tubulointerstitial damage remain unclear. Here, we investigated dietary iron restriction effect on tubulointerstitial damage in mice with protein-overload tubulointerstitial injury. Renal tubulointerstitial injury in animal model was induced by intraperitoneal injection of an overdose of bovine serum albumin (BSA). We divided mice into three groups: normal saline + normal diet (ND), BSA + ND, and BSA + iron-restricted diet (IRD). BSA overload induced renal tubulointerstitial injury in the ND mice, which was ameliorated in the IRD mice. Inflammatory cytokines and extracellular matrix mRNA expression was upregulated in BSA + ND mice kidneys and was inhibited by IRD. BSA-induced increase in renal superoxide production, NADPH oxidase activity, and p22(phox) expression was diminished in the IRD mice. IRD suppression increased BSA-induced renal macrophage infiltration. Moreover, BSA mice exhibited nucleotide-binding oligomerisation domain-like receptor pyrin domain-containing protein (NLRP) inflammasome activation, which was inhibited by IRD. Ferrous iron increased in kidneys with BSA overload and was inhibited by IRD. Thus, iron restriction inhibited oxidative stress and inflammatory changes, contributing to the protective effect against BSA overload-induced tubulointerstitial injury. Nature Publishing Group UK 2017-09-06 /pmc/articles/PMC5587788/ /pubmed/28878231 http://dx.doi.org/10.1038/s41598-017-11089-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ikeda, Yasumasa Horinouchi, Yuya Hamano, Hirofumi Hirayama, Tasuku Kishi, Seiji Izawa-Ishizawa, Yuki Imanishi, Masaki Zamami, Yoshito Takechi, Kenshi Miyamoto, Licht Ishizawa, Keisuke Aihara, Ken-ichi Nagasawa, Hideko Tsuchiya, Koichiro Tamaki, Toshiaki Dietary iron restriction alleviates renal tubulointerstitial injury induced by protein overload in mice |
title | Dietary iron restriction alleviates renal tubulointerstitial injury induced by protein overload in mice |
title_full | Dietary iron restriction alleviates renal tubulointerstitial injury induced by protein overload in mice |
title_fullStr | Dietary iron restriction alleviates renal tubulointerstitial injury induced by protein overload in mice |
title_full_unstemmed | Dietary iron restriction alleviates renal tubulointerstitial injury induced by protein overload in mice |
title_short | Dietary iron restriction alleviates renal tubulointerstitial injury induced by protein overload in mice |
title_sort | dietary iron restriction alleviates renal tubulointerstitial injury induced by protein overload in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587788/ https://www.ncbi.nlm.nih.gov/pubmed/28878231 http://dx.doi.org/10.1038/s41598-017-11089-0 |
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