Cargando…
The third restriction–modification system from Thermus aquaticus YT-1: solving the riddle of two TaqII specificities
Two restriction–modification systems have been previously discovered in Thermus aquaticus YT-1. TaqI is a 263-amino acid (aa) Type IIP restriction enzyme that recognizes and cleaves within the symmetric sequence 5′-TCGA-3′. TaqII, in contrast, is a 1105-aa Type IIC restriction-and-modification enzym...
Autores principales: | Skowron, Piotr M., Anton, Brian P., Czajkowska, Edyta, Zebrowska, Joanna, Sulecka, Ewa, Krefft, Daria, Jezewska-Frackowiak, Joanna, Zolnierkiewicz, Olga, Witkowska, Malgorzata, Morgan, Richard D., Wilson, Geoffrey G., Fomenkov, Alexey, Roberts, Richard J., Zylicz-Stachula, Agnieszka |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587805/ https://www.ncbi.nlm.nih.gov/pubmed/28911108 http://dx.doi.org/10.1093/nar/gkx599 |
Ejemplares similares
-
Modified ‘one amino acid-one codon’ engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase
por: Zylicz-Stachula, Agnieszka, et al.
Publicado: (2014) -
Bifunctional TaqII restriction endonuclease: redefining the prototype DNA recognition site and establishing the Fidelity Index for partial cleaving
por: Żylicz-Stachula, Agnieszka, et al.
Publicado: (2011) -
A new genomic tool, ultra-frequently cleaving TaqII/sinefungin endonuclease with a combined 2.9-bp recognition site, applied to the construction of horse DNA libraries
por: Zylicz-Stachula, Agnieszka, et al.
Publicado: (2013) -
An efficient method for the construction of artificial, concatemeric DNA, RNA and proteins with genetically programmed functions, using a novel, vector-enzymatic DNA fragment amplification-expression technology
por: Skowron, Piotr M., et al.
Publicado: (2020) -
Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus
por: Skowron, Piotr M, et al.
Publicado: (2013)