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Broad-Host-Range Expression Reveals Native and Host Regulatory Elements That Influence Heterologous Antibiotic Production in Gram-Negative Bacteria

Heterologous expression has become a powerful tool for studying microbial biosynthetic gene clusters (BGCs). Here, we extend the transformation-associated recombination cloning and heterologous expression platform for microbial BGCs to include Gram-negative proteobacterial expression hosts. Using a...

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Detalles Bibliográficos
Autores principales:	Zhang, Jia Jia, Tang, Xiaoyu, Zhang, Michelle, Nguyen, Darlene, Moore, Bradley S.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587914/ https://www.ncbi.nlm.nih.gov/pubmed/28874475 http://dx.doi.org/10.1128/mBio.01291-17

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