Cargando…

Quercetin inhibits angiogenesis-mediated human retinoblastoma growth by targeting vascular endothelial growth factor receptor

Retinoblastoma (RB) is the most common malignant intraocular cancer in teenagers, occurrence of which depends on the mutation of multiple genes. Among all the signaling pathways involved in the oncogenesis of RB, the process of angiogenesis has been demonstrated to be associated with the local invas...

Descripción completa

Detalles Bibliográficos
Autores principales: Song, Wei, Zhao, Xiaofei, Xu, Jiarui, Zhang, Han
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5588034/
https://www.ncbi.nlm.nih.gov/pubmed/28927086
http://dx.doi.org/10.3892/ol.2017.6623
_version_ 1783262101961179136
author Song, Wei
Zhao, Xiaofei
Xu, Jiarui
Zhang, Han
author_facet Song, Wei
Zhao, Xiaofei
Xu, Jiarui
Zhang, Han
author_sort Song, Wei
collection PubMed
description Retinoblastoma (RB) is the most common malignant intraocular cancer in teenagers, occurrence of which depends on the mutation of multiple genes. Among all the signaling pathways involved in the oncogenesis of RB, the process of angiogenesis has been demonstrated to be associated with the local invasive growth and metastasis of this cancer type. Quercetin (Que) is a typical flavonoid and has been reported to inhibit angiogenesis in various types of tumors. In the present study, the effect of Que on RB cells and angiogenesis of RB was evaluated. The human RB Y79 cell line was subjected to treatment with Que of various concentrations. Viability, invasion and migration ability and apoptosis of Y79 cells were subsequently measured to assess the effect of Que on RB cells. In addition, the expression of vascular endothelial growth factor receptor (VEGFR) was also quantified. It was revealed that Que inhibited RB cell growth and invasion in vitro in a dose-dependent manner, with 100 µM Que exhibiting the strongest inhibitory effect. In addition, Que downregulated the expression of VEGFR, which was an indicator of the blockade of angiogenesis in RB by targeting VEGF. The effect of Que on angiogenesis was also observed to be dose-dependent. The results of the present study indicated that Que may be a potential anti-RB therapy due to its anti-angiogenesis effect.
format Online
Article
Text
id pubmed-5588034
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-55880342017-09-18 Quercetin inhibits angiogenesis-mediated human retinoblastoma growth by targeting vascular endothelial growth factor receptor Song, Wei Zhao, Xiaofei Xu, Jiarui Zhang, Han Oncol Lett Articles Retinoblastoma (RB) is the most common malignant intraocular cancer in teenagers, occurrence of which depends on the mutation of multiple genes. Among all the signaling pathways involved in the oncogenesis of RB, the process of angiogenesis has been demonstrated to be associated with the local invasive growth and metastasis of this cancer type. Quercetin (Que) is a typical flavonoid and has been reported to inhibit angiogenesis in various types of tumors. In the present study, the effect of Que on RB cells and angiogenesis of RB was evaluated. The human RB Y79 cell line was subjected to treatment with Que of various concentrations. Viability, invasion and migration ability and apoptosis of Y79 cells were subsequently measured to assess the effect of Que on RB cells. In addition, the expression of vascular endothelial growth factor receptor (VEGFR) was also quantified. It was revealed that Que inhibited RB cell growth and invasion in vitro in a dose-dependent manner, with 100 µM Que exhibiting the strongest inhibitory effect. In addition, Que downregulated the expression of VEGFR, which was an indicator of the blockade of angiogenesis in RB by targeting VEGF. The effect of Que on angiogenesis was also observed to be dose-dependent. The results of the present study indicated that Que may be a potential anti-RB therapy due to its anti-angiogenesis effect. D.A. Spandidos 2017-09 2017-07-20 /pmc/articles/PMC5588034/ /pubmed/28927086 http://dx.doi.org/10.3892/ol.2017.6623 Text en Copyright: © Song et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Song, Wei
Zhao, Xiaofei
Xu, Jiarui
Zhang, Han
Quercetin inhibits angiogenesis-mediated human retinoblastoma growth by targeting vascular endothelial growth factor receptor
title Quercetin inhibits angiogenesis-mediated human retinoblastoma growth by targeting vascular endothelial growth factor receptor
title_full Quercetin inhibits angiogenesis-mediated human retinoblastoma growth by targeting vascular endothelial growth factor receptor
title_fullStr Quercetin inhibits angiogenesis-mediated human retinoblastoma growth by targeting vascular endothelial growth factor receptor
title_full_unstemmed Quercetin inhibits angiogenesis-mediated human retinoblastoma growth by targeting vascular endothelial growth factor receptor
title_short Quercetin inhibits angiogenesis-mediated human retinoblastoma growth by targeting vascular endothelial growth factor receptor
title_sort quercetin inhibits angiogenesis-mediated human retinoblastoma growth by targeting vascular endothelial growth factor receptor
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5588034/
https://www.ncbi.nlm.nih.gov/pubmed/28927086
http://dx.doi.org/10.3892/ol.2017.6623
work_keys_str_mv AT songwei quercetininhibitsangiogenesismediatedhumanretinoblastomagrowthbytargetingvascularendothelialgrowthfactorreceptor
AT zhaoxiaofei quercetininhibitsangiogenesismediatedhumanretinoblastomagrowthbytargetingvascularendothelialgrowthfactorreceptor
AT xujiarui quercetininhibitsangiogenesismediatedhumanretinoblastomagrowthbytargetingvascularendothelialgrowthfactorreceptor
AT zhanghan quercetininhibitsangiogenesismediatedhumanretinoblastomagrowthbytargetingvascularendothelialgrowthfactorreceptor