Cargando…

In vitro Adhesion of Oral Candida dubliniensis Isolates to Acrylic Denture Surfaces following Brief Exposure to Sub-Cidal Concentrations of Polyenes, Azoles and Chlorhexidine

OBJECTIVES: We aimed to investigate the effect of brief exposure to sub-cidal concentrations of nystatin, amphotericin B, ketoconazole, fluconazole and chlorhexidine gluconate on the adhesion of oral Candida dubliniensis isolates to the surface of acrylic dentures. METHODS: After determining the min...

Descripción completa

Detalles Bibliográficos
Autores principales: Ellepola, Arjuna N.B., Joseph, Bobby K., Altarakemah, Yacoub, Samaranayake, Lakshman P., Anil, Sukumaran, Hashem, Mohamed, Khan, Zia U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: S. Karger AG 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5588188/
https://www.ncbi.nlm.nih.gov/pubmed/25471106
http://dx.doi.org/10.1159/000369019
_version_ 1783262126291288064
author Ellepola, Arjuna N.B.
Joseph, Bobby K.
Altarakemah, Yacoub
Samaranayake, Lakshman P.
Anil, Sukumaran
Hashem, Mohamed
Khan, Zia U.
author_facet Ellepola, Arjuna N.B.
Joseph, Bobby K.
Altarakemah, Yacoub
Samaranayake, Lakshman P.
Anil, Sukumaran
Hashem, Mohamed
Khan, Zia U.
author_sort Ellepola, Arjuna N.B.
collection PubMed
description OBJECTIVES: We aimed to investigate the effect of brief exposure to sub-cidal concentrations of nystatin, amphotericin B, ketoconazole, fluconazole and chlorhexidine gluconate on the adhesion of oral Candida dubliniensis isolates to the surface of acrylic dentures. METHODS: After determining the minimum inhibitory concentration of each drug, 20 oral isolates of C. dubliniensis were exposed to sub-cidal concentrations of the drugs for 1 h. The drugs were then removed by dilution, and the adhesion of the isolates to denture acrylic strips was assessed by an in vitro adhesion assay. RESULTS: Compared to the controls, exposure to nystatin, amphotericin B, ketoconazole, fluconazole and chlorhexidine gluconate suppressed the ability of C. dubliniensis isolates to adhere to acrylic denture surfaces with a reduction of 74.68, 74.27, 57.31, 44.57 and 56.53% (p < 0.001 for all drugs), respectively. CONCLUSIONS: Brief exposure to sub-cidal concentrations of anti-mycotics suppressed the adhesion of C. dubliniensis oral isolates to acrylic denture surfaces.
format Online
Article
Text
id pubmed-5588188
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher S. Karger AG
record_format MEDLINE/PubMed
spelling pubmed-55881882017-11-01 In vitro Adhesion of Oral Candida dubliniensis Isolates to Acrylic Denture Surfaces following Brief Exposure to Sub-Cidal Concentrations of Polyenes, Azoles and Chlorhexidine Ellepola, Arjuna N.B. Joseph, Bobby K. Altarakemah, Yacoub Samaranayake, Lakshman P. Anil, Sukumaran Hashem, Mohamed Khan, Zia U. Med Princ Pract Original Paper OBJECTIVES: We aimed to investigate the effect of brief exposure to sub-cidal concentrations of nystatin, amphotericin B, ketoconazole, fluconazole and chlorhexidine gluconate on the adhesion of oral Candida dubliniensis isolates to the surface of acrylic dentures. METHODS: After determining the minimum inhibitory concentration of each drug, 20 oral isolates of C. dubliniensis were exposed to sub-cidal concentrations of the drugs for 1 h. The drugs were then removed by dilution, and the adhesion of the isolates to denture acrylic strips was assessed by an in vitro adhesion assay. RESULTS: Compared to the controls, exposure to nystatin, amphotericin B, ketoconazole, fluconazole and chlorhexidine gluconate suppressed the ability of C. dubliniensis isolates to adhere to acrylic denture surfaces with a reduction of 74.68, 74.27, 57.31, 44.57 and 56.53% (p < 0.001 for all drugs), respectively. CONCLUSIONS: Brief exposure to sub-cidal concentrations of anti-mycotics suppressed the adhesion of C. dubliniensis oral isolates to acrylic denture surfaces. S. Karger AG 2015-01 2014-11-29 /pmc/articles/PMC5588188/ /pubmed/25471106 http://dx.doi.org/10.1159/000369019 Text en Copyright © 2014 by S. Karger AG, Basel http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article licensed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported license (CC BY-NC) (www.karger.com/OA-license), applicable to the online version of the article only. Distribution permitted for non-commercial purposes only.
spellingShingle Original Paper
Ellepola, Arjuna N.B.
Joseph, Bobby K.
Altarakemah, Yacoub
Samaranayake, Lakshman P.
Anil, Sukumaran
Hashem, Mohamed
Khan, Zia U.
In vitro Adhesion of Oral Candida dubliniensis Isolates to Acrylic Denture Surfaces following Brief Exposure to Sub-Cidal Concentrations of Polyenes, Azoles and Chlorhexidine
title In vitro Adhesion of Oral Candida dubliniensis Isolates to Acrylic Denture Surfaces following Brief Exposure to Sub-Cidal Concentrations of Polyenes, Azoles and Chlorhexidine
title_full In vitro Adhesion of Oral Candida dubliniensis Isolates to Acrylic Denture Surfaces following Brief Exposure to Sub-Cidal Concentrations of Polyenes, Azoles and Chlorhexidine
title_fullStr In vitro Adhesion of Oral Candida dubliniensis Isolates to Acrylic Denture Surfaces following Brief Exposure to Sub-Cidal Concentrations of Polyenes, Azoles and Chlorhexidine
title_full_unstemmed In vitro Adhesion of Oral Candida dubliniensis Isolates to Acrylic Denture Surfaces following Brief Exposure to Sub-Cidal Concentrations of Polyenes, Azoles and Chlorhexidine
title_short In vitro Adhesion of Oral Candida dubliniensis Isolates to Acrylic Denture Surfaces following Brief Exposure to Sub-Cidal Concentrations of Polyenes, Azoles and Chlorhexidine
title_sort in vitro adhesion of oral candida dubliniensis isolates to acrylic denture surfaces following brief exposure to sub-cidal concentrations of polyenes, azoles and chlorhexidine
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5588188/
https://www.ncbi.nlm.nih.gov/pubmed/25471106
http://dx.doi.org/10.1159/000369019
work_keys_str_mv AT ellepolaarjunanb invitroadhesionoforalcandidadubliniensisisolatestoacrylicdenturesurfacesfollowingbriefexposuretosubcidalconcentrationsofpolyenesazolesandchlorhexidine
AT josephbobbyk invitroadhesionoforalcandidadubliniensisisolatestoacrylicdenturesurfacesfollowingbriefexposuretosubcidalconcentrationsofpolyenesazolesandchlorhexidine
AT altarakemahyacoub invitroadhesionoforalcandidadubliniensisisolatestoacrylicdenturesurfacesfollowingbriefexposuretosubcidalconcentrationsofpolyenesazolesandchlorhexidine
AT samaranayakelakshmanp invitroadhesionoforalcandidadubliniensisisolatestoacrylicdenturesurfacesfollowingbriefexposuretosubcidalconcentrationsofpolyenesazolesandchlorhexidine
AT anilsukumaran invitroadhesionoforalcandidadubliniensisisolatestoacrylicdenturesurfacesfollowingbriefexposuretosubcidalconcentrationsofpolyenesazolesandchlorhexidine
AT hashemmohamed invitroadhesionoforalcandidadubliniensisisolatestoacrylicdenturesurfacesfollowingbriefexposuretosubcidalconcentrationsofpolyenesazolesandchlorhexidine
AT khanziau invitroadhesionoforalcandidadubliniensisisolatestoacrylicdenturesurfacesfollowingbriefexposuretosubcidalconcentrationsofpolyenesazolesandchlorhexidine