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Anti-Inflammation of N-Benzyl-4-Bromobenzamide in Lipopolysaccharide-Induced Human Gingival Fibroblasts
OBJECTIVE: To evaluate the effect of N-benzyl-4-bromobenzamide (NBBA) on lipopolysaccharide (LPS)-induced IL-6 and prostaglandin E(2) (PGE(2)) production in human gingival fibroblasts (HGFs). MATERIAL AND METHODS: The benzamide compound was synthesized. The condition for IL-6 production of HGFs afte...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
S. Karger AG
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5588337/ https://www.ncbi.nlm.nih.gov/pubmed/26536614 http://dx.doi.org/10.1159/000442164 |
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author | Aroonrerk, Nuntana Niyomtham, Nattisa Yingyoungnarongkul, Boon-ek |
author_facet | Aroonrerk, Nuntana Niyomtham, Nattisa Yingyoungnarongkul, Boon-ek |
author_sort | Aroonrerk, Nuntana |
collection | PubMed |
description | OBJECTIVE: To evaluate the effect of N-benzyl-4-bromobenzamide (NBBA) on lipopolysaccharide (LPS)-induced IL-6 and prostaglandin E(2) (PGE(2)) production in human gingival fibroblasts (HGFs). MATERIAL AND METHODS: The benzamide compound was synthesized. The condition for IL-6 production of HGFs after induction with LPS was optimized. The HGFs were incubated with NBBA (10 µg/ml) for 30 min before LPS (1 μg/ml) was added. After 24 h of incubation time, the culture media were harvested and their IL-6 and PGE(2) contents were determined using an enzyme-linked immunosorbent assay. Prednisolone (PDS) and NS-398 were used as positive controls. Statistical analysis of the IL-6 and PGE(2) contents was performed using the ANOVA test followed by the Tukey multiple-comparisons test to compare replicate means. p < 0.001 was considered statistically significant. RESULTS: The maximum IL-6 production was achieved when HGFs were exposed to 1 μg/ml of LPS for 24 h, which was inhibited by the IL-6 immunosuppressant PDS. The benzamide compound, NBBA, exhibited a potent anti-IL-6 activity with inhibition of 35.6 ± 0.5%, significantly different from in the LPS-induced HGFs (p < 0.001). In addition, it inhibited 75.6 ± 0.52% PGE(2) production. Cell viability was not significantly affected by treatment with NBBA at a concentration <10 µg/ml (p < 0.001). CONCLUSIONS: NBBA exhibited an inhibitory effect on the production of IL-6 and PGE(2) in LPS-induced HGFs. It could serve as a compound with inhibiting inflammatory activity in periodontal disease. |
format | Online Article Text |
id | pubmed-5588337 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | S. Karger AG |
record_format | MEDLINE/PubMed |
spelling | pubmed-55883372017-11-01 Anti-Inflammation of N-Benzyl-4-Bromobenzamide in Lipopolysaccharide-Induced Human Gingival Fibroblasts Aroonrerk, Nuntana Niyomtham, Nattisa Yingyoungnarongkul, Boon-ek Med Princ Pract Original Paper OBJECTIVE: To evaluate the effect of N-benzyl-4-bromobenzamide (NBBA) on lipopolysaccharide (LPS)-induced IL-6 and prostaglandin E(2) (PGE(2)) production in human gingival fibroblasts (HGFs). MATERIAL AND METHODS: The benzamide compound was synthesized. The condition for IL-6 production of HGFs after induction with LPS was optimized. The HGFs were incubated with NBBA (10 µg/ml) for 30 min before LPS (1 μg/ml) was added. After 24 h of incubation time, the culture media were harvested and their IL-6 and PGE(2) contents were determined using an enzyme-linked immunosorbent assay. Prednisolone (PDS) and NS-398 were used as positive controls. Statistical analysis of the IL-6 and PGE(2) contents was performed using the ANOVA test followed by the Tukey multiple-comparisons test to compare replicate means. p < 0.001 was considered statistically significant. RESULTS: The maximum IL-6 production was achieved when HGFs were exposed to 1 μg/ml of LPS for 24 h, which was inhibited by the IL-6 immunosuppressant PDS. The benzamide compound, NBBA, exhibited a potent anti-IL-6 activity with inhibition of 35.6 ± 0.5%, significantly different from in the LPS-induced HGFs (p < 0.001). In addition, it inhibited 75.6 ± 0.52% PGE(2) production. Cell viability was not significantly affected by treatment with NBBA at a concentration <10 µg/ml (p < 0.001). CONCLUSIONS: NBBA exhibited an inhibitory effect on the production of IL-6 and PGE(2) in LPS-induced HGFs. It could serve as a compound with inhibiting inflammatory activity in periodontal disease. S. Karger AG 2016-02 2015-11-04 /pmc/articles/PMC5588337/ /pubmed/26536614 http://dx.doi.org/10.1159/000442164 Text en Copyright © 2015 by S. Karger AG, Basel http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article licensed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported license (CC BY-NC) (www.karger.com/OA-license), applicable to the online version of the article only. Distribution permitted for non-commercial purposes only. |
spellingShingle | Original Paper Aroonrerk, Nuntana Niyomtham, Nattisa Yingyoungnarongkul, Boon-ek Anti-Inflammation of N-Benzyl-4-Bromobenzamide in Lipopolysaccharide-Induced Human Gingival Fibroblasts |
title | Anti-Inflammation of N-Benzyl-4-Bromobenzamide in Lipopolysaccharide-Induced Human Gingival Fibroblasts |
title_full | Anti-Inflammation of N-Benzyl-4-Bromobenzamide in Lipopolysaccharide-Induced Human Gingival Fibroblasts |
title_fullStr | Anti-Inflammation of N-Benzyl-4-Bromobenzamide in Lipopolysaccharide-Induced Human Gingival Fibroblasts |
title_full_unstemmed | Anti-Inflammation of N-Benzyl-4-Bromobenzamide in Lipopolysaccharide-Induced Human Gingival Fibroblasts |
title_short | Anti-Inflammation of N-Benzyl-4-Bromobenzamide in Lipopolysaccharide-Induced Human Gingival Fibroblasts |
title_sort | anti-inflammation of n-benzyl-4-bromobenzamide in lipopolysaccharide-induced human gingival fibroblasts |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5588337/ https://www.ncbi.nlm.nih.gov/pubmed/26536614 http://dx.doi.org/10.1159/000442164 |
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