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Identification of Nidogen 1 as a lung metastasis protein through secretome analysis
Secreted proteins play crucial roles in mediating tumor–stroma interactions during metastasis of cancer to different target organs. To comprehensively profile secreted proteins involved in lung metastasis, we applied quantitative mass spectrometry-based proteomics and identified 392 breast cancer-de...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5588926/ https://www.ncbi.nlm.nih.gov/pubmed/28827399 http://dx.doi.org/10.1101/gad.301937.117 |
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author | Alečković, Maša Wei, Yong LeRoy, Gary Sidoli, Simone Liu, Daniel D. Garcia, Benjamin A. Kang, Yibin |
author_facet | Alečković, Maša Wei, Yong LeRoy, Gary Sidoli, Simone Liu, Daniel D. Garcia, Benjamin A. Kang, Yibin |
author_sort | Alečković, Maša |
collection | PubMed |
description | Secreted proteins play crucial roles in mediating tumor–stroma interactions during metastasis of cancer to different target organs. To comprehensively profile secreted proteins involved in lung metastasis, we applied quantitative mass spectrometry-based proteomics and identified 392 breast cancer-derived and 302 melanoma-derived proteins secreted from highly lung metastatic cells. The cancer-specific lung metastasis secretome signatures (LMSSs) displayed significant prognostic value in multiple cancer clinical data sets. Moreover, we observed a significant overlap of enriched pathways between the LMSSs of breast cancer and melanoma despite an overall small overlap of specific proteins, suggesting that common biological processes are executed by different proteins to enable the two cancer types to metastasize to the lung. Among the novel candidate lung metastasis proteins, Nidogen 1 (NID1) was confirmed to promote lung metastasis of breast cancer and melanoma, and its expression is correlated with poor clinical outcomes. In vitro functional analysis further revealed multiple prometastatic functions of NID1, including enhancing cancer cell migration and invasion, promoting adhesion to the endothelium and disrupting its integrity, and improving vascular tube formation capacity. As a secreted prometastatic protein, NID1 may be developed as a new biomarker for disease progression and therapeutic target in breast cancer and melanoma. |
format | Online Article Text |
id | pubmed-5588926 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-55889262018-01-15 Identification of Nidogen 1 as a lung metastasis protein through secretome analysis Alečković, Maša Wei, Yong LeRoy, Gary Sidoli, Simone Liu, Daniel D. Garcia, Benjamin A. Kang, Yibin Genes Dev Research Paper Secreted proteins play crucial roles in mediating tumor–stroma interactions during metastasis of cancer to different target organs. To comprehensively profile secreted proteins involved in lung metastasis, we applied quantitative mass spectrometry-based proteomics and identified 392 breast cancer-derived and 302 melanoma-derived proteins secreted from highly lung metastatic cells. The cancer-specific lung metastasis secretome signatures (LMSSs) displayed significant prognostic value in multiple cancer clinical data sets. Moreover, we observed a significant overlap of enriched pathways between the LMSSs of breast cancer and melanoma despite an overall small overlap of specific proteins, suggesting that common biological processes are executed by different proteins to enable the two cancer types to metastasize to the lung. Among the novel candidate lung metastasis proteins, Nidogen 1 (NID1) was confirmed to promote lung metastasis of breast cancer and melanoma, and its expression is correlated with poor clinical outcomes. In vitro functional analysis further revealed multiple prometastatic functions of NID1, including enhancing cancer cell migration and invasion, promoting adhesion to the endothelium and disrupting its integrity, and improving vascular tube formation capacity. As a secreted prometastatic protein, NID1 may be developed as a new biomarker for disease progression and therapeutic target in breast cancer and melanoma. Cold Spring Harbor Laboratory Press 2017-07-15 /pmc/articles/PMC5588926/ /pubmed/28827399 http://dx.doi.org/10.1101/gad.301937.117 Text en © 2017 Alečković et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Research Paper Alečković, Maša Wei, Yong LeRoy, Gary Sidoli, Simone Liu, Daniel D. Garcia, Benjamin A. Kang, Yibin Identification of Nidogen 1 as a lung metastasis protein through secretome analysis |
title | Identification of Nidogen 1 as a lung metastasis protein through secretome analysis |
title_full | Identification of Nidogen 1 as a lung metastasis protein through secretome analysis |
title_fullStr | Identification of Nidogen 1 as a lung metastasis protein through secretome analysis |
title_full_unstemmed | Identification of Nidogen 1 as a lung metastasis protein through secretome analysis |
title_short | Identification of Nidogen 1 as a lung metastasis protein through secretome analysis |
title_sort | identification of nidogen 1 as a lung metastasis protein through secretome analysis |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5588926/ https://www.ncbi.nlm.nih.gov/pubmed/28827399 http://dx.doi.org/10.1101/gad.301937.117 |
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