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Low-density lipoproteins investigated under high hydrostatic pressure by elastic incoherent neutron scattering
Human low-density lipoprotein (LDL) is a highly complex nano-particle built up of various lipid classes and a single large protein moiety (apoB-100) owning essential physiological functions in the human body. Besides its vital role as a supplier of cholesterol and fat for peripheral tissues and cell...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5589066/ https://www.ncbi.nlm.nih.gov/pubmed/28733727 http://dx.doi.org/10.1140/epje/i2017-11558-8 |
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author | Peters, J. Martinez, N. Lehofer, B. Prassl, R. |
author_facet | Peters, J. Martinez, N. Lehofer, B. Prassl, R. |
author_sort | Peters, J. |
collection | PubMed |
description | Human low-density lipoprotein (LDL) is a highly complex nano-particle built up of various lipid classes and a single large protein moiety (apoB-100) owning essential physiological functions in the human body. Besides its vital role as a supplier of cholesterol and fat for peripheral tissues and cells, it is also a known key player in the formation of atherosclerosis. Due to these important roles in physiology and pathology the elucidation of structural and dynamical details is of great interest. In the current study we drew a broader picture of LDL dynamics using elastic incoherent neutron scattering (EINS) as a function of specified temperature and pressure points. We not only investigated a normolipidemic LDL sample, but also a triglyceride-rich and an oxidized one to mimic pathologic conditions as found under hyperlipidemic conditions or in atherosclerotic plaques, respectively. We could show that pressure has a significant effect on atomic motions in modified forms of LDL, whereas the normolipidemic sample seems to cope much better with high-pressure conditions irrespective of temperature. These findings might be explained by the altered lipid composition, which is either caused through elevated triglyceride content or modifications through lipid peroxidation. |
format | Online Article Text |
id | pubmed-5589066 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
record_format | MEDLINE/PubMed |
spelling | pubmed-55890662017-09-07 Low-density lipoproteins investigated under high hydrostatic pressure by elastic incoherent neutron scattering Peters, J. Martinez, N. Lehofer, B. Prassl, R. Eur Phys J E Soft Matter Article Human low-density lipoprotein (LDL) is a highly complex nano-particle built up of various lipid classes and a single large protein moiety (apoB-100) owning essential physiological functions in the human body. Besides its vital role as a supplier of cholesterol and fat for peripheral tissues and cells, it is also a known key player in the formation of atherosclerosis. Due to these important roles in physiology and pathology the elucidation of structural and dynamical details is of great interest. In the current study we drew a broader picture of LDL dynamics using elastic incoherent neutron scattering (EINS) as a function of specified temperature and pressure points. We not only investigated a normolipidemic LDL sample, but also a triglyceride-rich and an oxidized one to mimic pathologic conditions as found under hyperlipidemic conditions or in atherosclerotic plaques, respectively. We could show that pressure has a significant effect on atomic motions in modified forms of LDL, whereas the normolipidemic sample seems to cope much better with high-pressure conditions irrespective of temperature. These findings might be explained by the altered lipid composition, which is either caused through elevated triglyceride content or modifications through lipid peroxidation. 2017-07-26 2017-07 /pmc/articles/PMC5589066/ /pubmed/28733727 http://dx.doi.org/10.1140/epje/i2017-11558-8 Text en This article is published with open access at Springerlink.com (https://link.springer.com/) http://creativecommons.org/licenses/by/4.0 Open Access This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Peters, J. Martinez, N. Lehofer, B. Prassl, R. Low-density lipoproteins investigated under high hydrostatic pressure by elastic incoherent neutron scattering |
title | Low-density lipoproteins investigated under high hydrostatic pressure by elastic incoherent neutron scattering |
title_full | Low-density lipoproteins investigated under high hydrostatic pressure by elastic incoherent neutron scattering |
title_fullStr | Low-density lipoproteins investigated under high hydrostatic pressure by elastic incoherent neutron scattering |
title_full_unstemmed | Low-density lipoproteins investigated under high hydrostatic pressure by elastic incoherent neutron scattering |
title_short | Low-density lipoproteins investigated under high hydrostatic pressure by elastic incoherent neutron scattering |
title_sort | low-density lipoproteins investigated under high hydrostatic pressure by elastic incoherent neutron scattering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5589066/ https://www.ncbi.nlm.nih.gov/pubmed/28733727 http://dx.doi.org/10.1140/epje/i2017-11558-8 |
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