A thiol probe for measuring unfolded protein load and proteostasis in cells

When proteostasis becomes unbalanced, unfolded proteins can accumulate and aggregate. Here we report that the dye, tetraphenylethene maleimide (TPE-MI) can be used to measure cellular unfolded protein load. TPE-MI fluorescence is activated upon labelling free cysteine thiols, normally buried in the...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Moore Z., Moily, Nagaraj S., Bridgford, Jessica L., Wood, Rebecca J., Radwan, Mona, Smith, Trevor A., Song, Zhegang, Tang, Ben Zhong, Tilley, Leann, Xu, Xiaohong, Reid, Gavin E., Pouladi, Mahmoud A., Hong, Yuning, Hatters, Danny M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5589734/
https://www.ncbi.nlm.nih.gov/pubmed/28883394
http://dx.doi.org/10.1038/s41467-017-00203-5

Ejemplares similares

Ejemplares similares