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Alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers

Accumulation of glycine receptors at synapses requires the interaction between the beta subunit of the receptor and the scaffold protein gephyrin. Here, we questioned whether different alpha subunits could modulate the receptors’ diffusion and propensity to cluster at spinal cord synapses. Using qua...

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Autores principales: Patrizio, A., Renner, M., Pizzarelli, R., Triller, A., Specht, C. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5589798/
https://www.ncbi.nlm.nih.gov/pubmed/28883437
http://dx.doi.org/10.1038/s41598-017-11264-3
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author Patrizio, A.
Renner, M.
Pizzarelli, R.
Triller, A.
Specht, C. G.
author_facet Patrizio, A.
Renner, M.
Pizzarelli, R.
Triller, A.
Specht, C. G.
author_sort Patrizio, A.
collection PubMed
description Accumulation of glycine receptors at synapses requires the interaction between the beta subunit of the receptor and the scaffold protein gephyrin. Here, we questioned whether different alpha subunits could modulate the receptors’ diffusion and propensity to cluster at spinal cord synapses. Using quantitative photoactivated localisation microscopy we found that alpha-1 and alpha-3 containing glycine receptors display the same α(3):β(2) stoichiometry and gephyrin binding. Despite these similarities, alpha-3 containing receptors are less mobile and cluster at higher density compared to alpha-1, with 1500 versus 1100 complexes µm(−2), respectively. Furthermore, we identified a subunit-specific regulation of glycine receptor copy numbers at synapses: when challenged with interleukin 1β, the synaptic occupancy of alpha-1 but not alpha-3 receptors was reduced. This mechanism may play a role in the cell-type dependent regulation of glycinergic currents in response to interleukin 1β and highlights the capacity of the alpha subunits to affect receptor-gephyrin binding at synapses.
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spelling pubmed-55897982017-09-13 Alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers Patrizio, A. Renner, M. Pizzarelli, R. Triller, A. Specht, C. G. Sci Rep Article Accumulation of glycine receptors at synapses requires the interaction between the beta subunit of the receptor and the scaffold protein gephyrin. Here, we questioned whether different alpha subunits could modulate the receptors’ diffusion and propensity to cluster at spinal cord synapses. Using quantitative photoactivated localisation microscopy we found that alpha-1 and alpha-3 containing glycine receptors display the same α(3):β(2) stoichiometry and gephyrin binding. Despite these similarities, alpha-3 containing receptors are less mobile and cluster at higher density compared to alpha-1, with 1500 versus 1100 complexes µm(−2), respectively. Furthermore, we identified a subunit-specific regulation of glycine receptor copy numbers at synapses: when challenged with interleukin 1β, the synaptic occupancy of alpha-1 but not alpha-3 receptors was reduced. This mechanism may play a role in the cell-type dependent regulation of glycinergic currents in response to interleukin 1β and highlights the capacity of the alpha subunits to affect receptor-gephyrin binding at synapses. Nature Publishing Group UK 2017-09-07 /pmc/articles/PMC5589798/ /pubmed/28883437 http://dx.doi.org/10.1038/s41598-017-11264-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Patrizio, A.
Renner, M.
Pizzarelli, R.
Triller, A.
Specht, C. G.
Alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers
title Alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers
title_full Alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers
title_fullStr Alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers
title_full_unstemmed Alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers
title_short Alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers
title_sort alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5589798/
https://www.ncbi.nlm.nih.gov/pubmed/28883437
http://dx.doi.org/10.1038/s41598-017-11264-3
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