Integrated Method to Attach DNA Handles and Functionally Select Proteins to Study Folding and Protein-Ligand Interactions with Optical Tweezers

Optical tweezers has emerged as a powerful tool to study folding, ligand binding, and motor enzymes. The manipulation of proteins with optical tweezers requires attaching molecular handles to the protein of interest. Here, we describe a novel method that integrates the covalent attachment of DNA han...

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Detalles Bibliográficos
Autores principales: Hao, Yuxin, Canavan, Clare, Taylor, Susan S., Maillard, Rodrigo A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5589850/
https://www.ncbi.nlm.nih.gov/pubmed/28883488
http://dx.doi.org/10.1038/s41598-017-11214-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5589850/
https://www.ncbi.nlm.nih.gov/pubmed/28883488
http://dx.doi.org/10.1038/s41598-017-11214-z

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