Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids

Artificial metalloenzymes have emerged as an attractive new approach to enantioselective catalysis. Herein, we introduce a novel strategy for preparation of artificial metalloenzymes utilizing amber stop codon suppression methodology for the in vivo incorporation of metal-binding unnatural amino aci...

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Autores principales: Drienovská, Ivana, Rioz-Martínez, Ana, Draksharapu, Apparao, Roelfes, Gerard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2015
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5590542/
https://www.ncbi.nlm.nih.gov/pubmed/28936318
http://dx.doi.org/10.1039/c4sc01525h
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author Drienovská, Ivana
Rioz-Martínez, Ana
Draksharapu, Apparao
Roelfes, Gerard
author_facet Drienovská, Ivana
Rioz-Martínez, Ana
Draksharapu, Apparao
Roelfes, Gerard
author_sort Drienovská, Ivana
collection PubMed
description Artificial metalloenzymes have emerged as an attractive new approach to enantioselective catalysis. Herein, we introduce a novel strategy for preparation of artificial metalloenzymes utilizing amber stop codon suppression methodology for the in vivo incorporation of metal-binding unnatural amino acids. The resulting artificial metalloenzymes were applied in catalytic asymmetric Friedel–Crafts alkylation reactions and up to 83% ee for the product was achieved.
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spelling pubmed-55905422017-09-21 Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids Drienovská, Ivana Rioz-Martínez, Ana Draksharapu, Apparao Roelfes, Gerard Chem Sci Chemistry Artificial metalloenzymes have emerged as an attractive new approach to enantioselective catalysis. Herein, we introduce a novel strategy for preparation of artificial metalloenzymes utilizing amber stop codon suppression methodology for the in vivo incorporation of metal-binding unnatural amino acids. The resulting artificial metalloenzymes were applied in catalytic asymmetric Friedel–Crafts alkylation reactions and up to 83% ee for the product was achieved. Royal Society of Chemistry 2015-01-01 2014-10-09 /pmc/articles/PMC5590542/ /pubmed/28936318 http://dx.doi.org/10.1039/c4sc01525h Text en This journal is © The Royal Society of Chemistry 2014 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Drienovská, Ivana
Rioz-Martínez, Ana
Draksharapu, Apparao
Roelfes, Gerard
Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids
title Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids
title_full Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids
title_fullStr Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids
title_full_unstemmed Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids
title_short Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids
title_sort novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5590542/
https://www.ncbi.nlm.nih.gov/pubmed/28936318
http://dx.doi.org/10.1039/c4sc01525h
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