Complete sequence-based pathway analysis by differential on-chip DNA and RNA extraction from a single cell

We demonstrate on-chip, differential DNA and RNA extraction from a single cell using a microfluidic chip and a two-stage lysis protocol. This method enables direct use of the whole extract, without additional washing steps, reducing sample loss. Using this method, the tumor driving pathway in indivi...

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Detalles Bibliográficos
Autores principales: van Strijp, D., Vulders, R. C. M., Larsen, N. A., Schira, J., Baerlocher, L., van Driel, M. A., Pødenphant, M., Hansen, T. S., Kristensen, A., Mir, K. U., Olesen, T., Verhaegh, W. F. J., Marie, R., van der Zaag, P. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5591254/
https://www.ncbi.nlm.nih.gov/pubmed/28887473
http://dx.doi.org/10.1038/s41598-017-10704-4

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