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The salivary microbiome is consistent between subjects and resistant to impacts of short-term hospitalization

In recent years, a growing amount of research has begun to focus on the oral microbiome due to its links with health and systemic disease. The oral microbiome has numerous advantages that make it particularly useful for clinical studies, including non-invasive collection, temporal stability, and low...

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Autores principales: Cabral, Damien J., Wurster, Jenna I., Flokas, Myrto E., Alevizakos, Michail, Zabat, Michelle, Korry, Benjamin J., Rowan, Aislinn D., Sano, William H., Andreatos, Nikolaos, Ducharme, R. Bobby, Chan, Philip A., Mylonakis, Eleftherios, Fuchs, Beth Burgwyn, Belenky, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5591268/
https://www.ncbi.nlm.nih.gov/pubmed/28887570
http://dx.doi.org/10.1038/s41598-017-11427-2
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author Cabral, Damien J.
Wurster, Jenna I.
Flokas, Myrto E.
Alevizakos, Michail
Zabat, Michelle
Korry, Benjamin J.
Rowan, Aislinn D.
Sano, William H.
Andreatos, Nikolaos
Ducharme, R. Bobby
Chan, Philip A.
Mylonakis, Eleftherios
Fuchs, Beth Burgwyn
Belenky, Peter
author_facet Cabral, Damien J.
Wurster, Jenna I.
Flokas, Myrto E.
Alevizakos, Michail
Zabat, Michelle
Korry, Benjamin J.
Rowan, Aislinn D.
Sano, William H.
Andreatos, Nikolaos
Ducharme, R. Bobby
Chan, Philip A.
Mylonakis, Eleftherios
Fuchs, Beth Burgwyn
Belenky, Peter
author_sort Cabral, Damien J.
collection PubMed
description In recent years, a growing amount of research has begun to focus on the oral microbiome due to its links with health and systemic disease. The oral microbiome has numerous advantages that make it particularly useful for clinical studies, including non-invasive collection, temporal stability, and lower complexity relative to other niches, such as the gut. Despite recent discoveries made in this area, it is unknown how the oral microbiome responds to short-term hospitalization. Previous studies have demonstrated that the gut microbiome is extremely sensitive to short-term hospitalization and that these changes are associated with significant morbidity and mortality. Here, we present a comprehensive pipeline for reliable bedside collection, sequencing, and analysis of the human salivary microbiome. We also develop a novel oral-specific mock community for pipeline validation. Using our methodology, we analyzed the salivary microbiomes of patients before and during hospitalization or azithromycin treatment to profile impacts on this community. Our findings indicate that azithromycin alters the diversity and taxonomic composition of the salivary microbiome; however, we also found that short-term hospitalization does not impact the richness or structure of this community, suggesting that the oral cavity may be less susceptible to dysbiosis during short-term hospitalization.
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spelling pubmed-55912682017-09-13 The salivary microbiome is consistent between subjects and resistant to impacts of short-term hospitalization Cabral, Damien J. Wurster, Jenna I. Flokas, Myrto E. Alevizakos, Michail Zabat, Michelle Korry, Benjamin J. Rowan, Aislinn D. Sano, William H. Andreatos, Nikolaos Ducharme, R. Bobby Chan, Philip A. Mylonakis, Eleftherios Fuchs, Beth Burgwyn Belenky, Peter Sci Rep Article In recent years, a growing amount of research has begun to focus on the oral microbiome due to its links with health and systemic disease. The oral microbiome has numerous advantages that make it particularly useful for clinical studies, including non-invasive collection, temporal stability, and lower complexity relative to other niches, such as the gut. Despite recent discoveries made in this area, it is unknown how the oral microbiome responds to short-term hospitalization. Previous studies have demonstrated that the gut microbiome is extremely sensitive to short-term hospitalization and that these changes are associated with significant morbidity and mortality. Here, we present a comprehensive pipeline for reliable bedside collection, sequencing, and analysis of the human salivary microbiome. We also develop a novel oral-specific mock community for pipeline validation. Using our methodology, we analyzed the salivary microbiomes of patients before and during hospitalization or azithromycin treatment to profile impacts on this community. Our findings indicate that azithromycin alters the diversity and taxonomic composition of the salivary microbiome; however, we also found that short-term hospitalization does not impact the richness or structure of this community, suggesting that the oral cavity may be less susceptible to dysbiosis during short-term hospitalization. Nature Publishing Group UK 2017-09-08 /pmc/articles/PMC5591268/ /pubmed/28887570 http://dx.doi.org/10.1038/s41598-017-11427-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Cabral, Damien J.
Wurster, Jenna I.
Flokas, Myrto E.
Alevizakos, Michail
Zabat, Michelle
Korry, Benjamin J.
Rowan, Aislinn D.
Sano, William H.
Andreatos, Nikolaos
Ducharme, R. Bobby
Chan, Philip A.
Mylonakis, Eleftherios
Fuchs, Beth Burgwyn
Belenky, Peter
The salivary microbiome is consistent between subjects and resistant to impacts of short-term hospitalization
title The salivary microbiome is consistent between subjects and resistant to impacts of short-term hospitalization
title_full The salivary microbiome is consistent between subjects and resistant to impacts of short-term hospitalization
title_fullStr The salivary microbiome is consistent between subjects and resistant to impacts of short-term hospitalization
title_full_unstemmed The salivary microbiome is consistent between subjects and resistant to impacts of short-term hospitalization
title_short The salivary microbiome is consistent between subjects and resistant to impacts of short-term hospitalization
title_sort salivary microbiome is consistent between subjects and resistant to impacts of short-term hospitalization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5591268/
https://www.ncbi.nlm.nih.gov/pubmed/28887570
http://dx.doi.org/10.1038/s41598-017-11427-2
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