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Integration of deep transcriptome and proteome analyses of salicylic acid regulation high temperature stress in Ulva prolifera
To investigate changes in transcript and relative protein levels in response to salicylic acid regulation of the thermotolerance in U. prolifera, complementary transcriptome and proteome analyses were performed with U. prolifera grown at 35 °C (UpHT) and with the addition of SA at high temperature (...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5591278/ https://www.ncbi.nlm.nih.gov/pubmed/28887495 http://dx.doi.org/10.1038/s41598-017-11449-w |
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author | Fan, Meihua Sun, Xue Xu, Nianjun Liao, Zhi Li, Yahe Wang, Jianxin Fan, Yingping Cui, Dalian Li, Peng Miao, Zengliang |
author_facet | Fan, Meihua Sun, Xue Xu, Nianjun Liao, Zhi Li, Yahe Wang, Jianxin Fan, Yingping Cui, Dalian Li, Peng Miao, Zengliang |
author_sort | Fan, Meihua |
collection | PubMed |
description | To investigate changes in transcript and relative protein levels in response to salicylic acid regulation of the thermotolerance in U. prolifera, complementary transcriptome and proteome analyses were performed with U. prolifera grown at 35 °C (UpHT) and with the addition of SA at high temperature (UpSHT). At mRNA level,12,296 differentially expressed genes (DEGs) were obtained from the comparison of UpSHT with UpHT. iTRAQ-labeling proteome analysis showed that a total of 4,449 proteins were identified and reliably quantified. At mRNA level, the up-regulated genes involved in antioxidant activity were thioredoxin,peroxiredoxin,FeSOD, glutathione peroxidase, partion catalase and MnSOD. The down-regulated genes were ascorbate peroxidase, glutathione S-transferase, catalase and MnSOD. In addition, the DEGs involved in plant signal transduction pathway (such as auxin response factors, BRI1 and JAZ) were down-regulated. At protein level, the up-regulated proteins involved in carbon fixation and the down-regulated protein mainly were polyubiquitin, ascorbate peroxidase. The expression of Ca(2+)-binding protein, heat shock protein and photosynthesis-related proteins, EDS1 were also significantly regulated both at mRNA and protein level. The results indicated that SA alleviated the high-temperature stimulus through partion antioxidant related proteins up-regulated, JA signal pathway enchanced, Ca(2+)-binding proteins, photosynthesis-related proteins significantly changed, antioxidant enzyme activities increased and photosynthesis index changed. |
format | Online Article Text |
id | pubmed-5591278 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-55912782017-09-13 Integration of deep transcriptome and proteome analyses of salicylic acid regulation high temperature stress in Ulva prolifera Fan, Meihua Sun, Xue Xu, Nianjun Liao, Zhi Li, Yahe Wang, Jianxin Fan, Yingping Cui, Dalian Li, Peng Miao, Zengliang Sci Rep Article To investigate changes in transcript and relative protein levels in response to salicylic acid regulation of the thermotolerance in U. prolifera, complementary transcriptome and proteome analyses were performed with U. prolifera grown at 35 °C (UpHT) and with the addition of SA at high temperature (UpSHT). At mRNA level,12,296 differentially expressed genes (DEGs) were obtained from the comparison of UpSHT with UpHT. iTRAQ-labeling proteome analysis showed that a total of 4,449 proteins were identified and reliably quantified. At mRNA level, the up-regulated genes involved in antioxidant activity were thioredoxin,peroxiredoxin,FeSOD, glutathione peroxidase, partion catalase and MnSOD. The down-regulated genes were ascorbate peroxidase, glutathione S-transferase, catalase and MnSOD. In addition, the DEGs involved in plant signal transduction pathway (such as auxin response factors, BRI1 and JAZ) were down-regulated. At protein level, the up-regulated proteins involved in carbon fixation and the down-regulated protein mainly were polyubiquitin, ascorbate peroxidase. The expression of Ca(2+)-binding protein, heat shock protein and photosynthesis-related proteins, EDS1 were also significantly regulated both at mRNA and protein level. The results indicated that SA alleviated the high-temperature stimulus through partion antioxidant related proteins up-regulated, JA signal pathway enchanced, Ca(2+)-binding proteins, photosynthesis-related proteins significantly changed, antioxidant enzyme activities increased and photosynthesis index changed. Nature Publishing Group UK 2017-09-08 /pmc/articles/PMC5591278/ /pubmed/28887495 http://dx.doi.org/10.1038/s41598-017-11449-w Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Fan, Meihua Sun, Xue Xu, Nianjun Liao, Zhi Li, Yahe Wang, Jianxin Fan, Yingping Cui, Dalian Li, Peng Miao, Zengliang Integration of deep transcriptome and proteome analyses of salicylic acid regulation high temperature stress in Ulva prolifera |
title | Integration of deep transcriptome and proteome analyses of salicylic acid regulation high temperature stress in Ulva prolifera |
title_full | Integration of deep transcriptome and proteome analyses of salicylic acid regulation high temperature stress in Ulva prolifera |
title_fullStr | Integration of deep transcriptome and proteome analyses of salicylic acid regulation high temperature stress in Ulva prolifera |
title_full_unstemmed | Integration of deep transcriptome and proteome analyses of salicylic acid regulation high temperature stress in Ulva prolifera |
title_short | Integration of deep transcriptome and proteome analyses of salicylic acid regulation high temperature stress in Ulva prolifera |
title_sort | integration of deep transcriptome and proteome analyses of salicylic acid regulation high temperature stress in ulva prolifera |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5591278/ https://www.ncbi.nlm.nih.gov/pubmed/28887495 http://dx.doi.org/10.1038/s41598-017-11449-w |
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