Cargando…
Bacterial microbiota associated with Rhipicephalus sanguineus (s.l.) ticks from France, Senegal and Arizona
BACKGROUND: Ticks of the group Rhipicephalus sanguineus (sensu lato) are distributed worldwide and are major pathogen vectors of both dogs and humans. Previous phylogenetic reconstructions have suggested the existence of two main lineages within this group, “Tropical” and “Temperate”. Symbiotic inte...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5591579/ https://www.ncbi.nlm.nih.gov/pubmed/28886749 http://dx.doi.org/10.1186/s13071-017-2352-9 |
_version_ | 1783262743441178624 |
---|---|
author | René-Martellet, Magalie Minard, Guillaume Massot, Raphael Tran Van, Van Valiente Moro, Claire Chabanne, Luc Mavingui, Patrick |
author_facet | René-Martellet, Magalie Minard, Guillaume Massot, Raphael Tran Van, Van Valiente Moro, Claire Chabanne, Luc Mavingui, Patrick |
author_sort | René-Martellet, Magalie |
collection | PubMed |
description | BACKGROUND: Ticks of the group Rhipicephalus sanguineus (sensu lato) are distributed worldwide and are major pathogen vectors of both dogs and humans. Previous phylogenetic reconstructions have suggested the existence of two main lineages within this group, “Tropical” and “Temperate”. Symbiotic interactions contribute to vector development, survival, reproduction and competence. The diversity of microbial communities associated with different populations of R. sanguineus (s.l.) remains poorly characterized, however, this knowledge will aid in future studies of hosts-microbiota-pathogen interactions. To gain insight into the bacterial communities associated with R. sanguineus (s.l.) ticks, 40 specimens from France, Senegal and Arizona were analyzed by high-throughput 16S amplicon sequencing. All tick specimens were taxonomically classified using the mitochondrial 12S rDNA gene, which provides sufficient phylogenetic resolution to discriminate different lineages of R. sanguineus. RESULTS: Rhipicephalus sanguineus (s.l.) samples from Senegal belonged to the “Tropical” lineage, samples from France belonged to the “Temperate” lineage, whereas both lineages were identified in samples from Arizona. Regardless of origin, each bacterial microbiota was dominated by three genera: Coxiella, Rickettsia and Bacillus. Rickettsia and Coxiella were the two main genera found in females whereas males had a higher proportion of Bacillus. Significant differences of relative abundances were evidenced between specimens from different geographical origins. CONCLUSIONS: This study highlights differences in the microbiota composition within R. sanguineus (s.l.) specimens from different genotypes, genders and geographical origins. This knowledge will help in future studies of the symbiotic interactions, biology and vector competence of the R. sanguineus (s.l.) complex. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-017-2352-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5591579 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-55915792017-09-13 Bacterial microbiota associated with Rhipicephalus sanguineus (s.l.) ticks from France, Senegal and Arizona René-Martellet, Magalie Minard, Guillaume Massot, Raphael Tran Van, Van Valiente Moro, Claire Chabanne, Luc Mavingui, Patrick Parasit Vectors Research BACKGROUND: Ticks of the group Rhipicephalus sanguineus (sensu lato) are distributed worldwide and are major pathogen vectors of both dogs and humans. Previous phylogenetic reconstructions have suggested the existence of two main lineages within this group, “Tropical” and “Temperate”. Symbiotic interactions contribute to vector development, survival, reproduction and competence. The diversity of microbial communities associated with different populations of R. sanguineus (s.l.) remains poorly characterized, however, this knowledge will aid in future studies of hosts-microbiota-pathogen interactions. To gain insight into the bacterial communities associated with R. sanguineus (s.l.) ticks, 40 specimens from France, Senegal and Arizona were analyzed by high-throughput 16S amplicon sequencing. All tick specimens were taxonomically classified using the mitochondrial 12S rDNA gene, which provides sufficient phylogenetic resolution to discriminate different lineages of R. sanguineus. RESULTS: Rhipicephalus sanguineus (s.l.) samples from Senegal belonged to the “Tropical” lineage, samples from France belonged to the “Temperate” lineage, whereas both lineages were identified in samples from Arizona. Regardless of origin, each bacterial microbiota was dominated by three genera: Coxiella, Rickettsia and Bacillus. Rickettsia and Coxiella were the two main genera found in females whereas males had a higher proportion of Bacillus. Significant differences of relative abundances were evidenced between specimens from different geographical origins. CONCLUSIONS: This study highlights differences in the microbiota composition within R. sanguineus (s.l.) specimens from different genotypes, genders and geographical origins. This knowledge will help in future studies of the symbiotic interactions, biology and vector competence of the R. sanguineus (s.l.) complex. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-017-2352-9) contains supplementary material, which is available to authorized users. BioMed Central 2017-09-07 /pmc/articles/PMC5591579/ /pubmed/28886749 http://dx.doi.org/10.1186/s13071-017-2352-9 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research René-Martellet, Magalie Minard, Guillaume Massot, Raphael Tran Van, Van Valiente Moro, Claire Chabanne, Luc Mavingui, Patrick Bacterial microbiota associated with Rhipicephalus sanguineus (s.l.) ticks from France, Senegal and Arizona |
title | Bacterial microbiota associated with Rhipicephalus sanguineus (s.l.) ticks from France, Senegal and Arizona |
title_full | Bacterial microbiota associated with Rhipicephalus sanguineus (s.l.) ticks from France, Senegal and Arizona |
title_fullStr | Bacterial microbiota associated with Rhipicephalus sanguineus (s.l.) ticks from France, Senegal and Arizona |
title_full_unstemmed | Bacterial microbiota associated with Rhipicephalus sanguineus (s.l.) ticks from France, Senegal and Arizona |
title_short | Bacterial microbiota associated with Rhipicephalus sanguineus (s.l.) ticks from France, Senegal and Arizona |
title_sort | bacterial microbiota associated with rhipicephalus sanguineus (s.l.) ticks from france, senegal and arizona |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5591579/ https://www.ncbi.nlm.nih.gov/pubmed/28886749 http://dx.doi.org/10.1186/s13071-017-2352-9 |
work_keys_str_mv | AT renemartelletmagalie bacterialmicrobiotaassociatedwithrhipicephalussanguineusslticksfromfrancesenegalandarizona AT minardguillaume bacterialmicrobiotaassociatedwithrhipicephalussanguineusslticksfromfrancesenegalandarizona AT massotraphael bacterialmicrobiotaassociatedwithrhipicephalussanguineusslticksfromfrancesenegalandarizona AT tranvanvan bacterialmicrobiotaassociatedwithrhipicephalussanguineusslticksfromfrancesenegalandarizona AT valientemoroclaire bacterialmicrobiotaassociatedwithrhipicephalussanguineusslticksfromfrancesenegalandarizona AT chabanneluc bacterialmicrobiotaassociatedwithrhipicephalussanguineusslticksfromfrancesenegalandarizona AT mavinguipatrick bacterialmicrobiotaassociatedwithrhipicephalussanguineusslticksfromfrancesenegalandarizona |