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Vascular Tissue Engineering: Effects of Integrating Collagen into a PCL Based Nanofiber Material

The engineering of vascular grafts is a growing field in regenerative medicine. Although numerous attempts have been made, the current vascular grafts made of polyurethane (PU), Dacron®, or Teflon® still display unsatisfying results. Electrospinning of biopolymers and native proteins has been in the...

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Autores principales: Bertram, Ulf, Steiner, Dominik, Poppitz, Benjamin, Dippold, Dirk, Köhn, Katrin, Beier, Justus P., Detsch, Rainer, Boccaccini, Aldo R., Schubert, Dirk W., Horch, Raymund E., Arkudas, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5592415/
https://www.ncbi.nlm.nih.gov/pubmed/28932749
http://dx.doi.org/10.1155/2017/9616939
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author Bertram, Ulf
Steiner, Dominik
Poppitz, Benjamin
Dippold, Dirk
Köhn, Katrin
Beier, Justus P.
Detsch, Rainer
Boccaccini, Aldo R.
Schubert, Dirk W.
Horch, Raymund E.
Arkudas, Andreas
author_facet Bertram, Ulf
Steiner, Dominik
Poppitz, Benjamin
Dippold, Dirk
Köhn, Katrin
Beier, Justus P.
Detsch, Rainer
Boccaccini, Aldo R.
Schubert, Dirk W.
Horch, Raymund E.
Arkudas, Andreas
author_sort Bertram, Ulf
collection PubMed
description The engineering of vascular grafts is a growing field in regenerative medicine. Although numerous attempts have been made, the current vascular grafts made of polyurethane (PU), Dacron®, or Teflon® still display unsatisfying results. Electrospinning of biopolymers and native proteins has been in the focus of research to imitate the extracellular matrix (ECM) of vessels to produce a small caliber, off-the-shelf tissue engineered vascular graft (TEVG) as a substitute for poorly performing PU, Dacron, or Teflon prostheses. Blended poly-ε-caprolactone (PCL)/collagen grafts have shown promising results regarding biomechanical and cell supporting features. In order to find a suitable PCL/collagen blend, we fabricated plane electrospun PCL scaffolds using various collagen type I concentrations ranging from 5% to 75%. We analyzed biocompatibility and morphological aspects in vitro. Our results show beneficial features of collagen I integration regarding cell viability and functionality, but also adverse effects like the loss of a confluent monolayer at high concentrations of collagen. Furthermore, electrospun PCL scaffolds containing 25% collagen I seem to be ideal for engineering vascular grafts.
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spelling pubmed-55924152017-09-20 Vascular Tissue Engineering: Effects of Integrating Collagen into a PCL Based Nanofiber Material Bertram, Ulf Steiner, Dominik Poppitz, Benjamin Dippold, Dirk Köhn, Katrin Beier, Justus P. Detsch, Rainer Boccaccini, Aldo R. Schubert, Dirk W. Horch, Raymund E. Arkudas, Andreas Biomed Res Int Research Article The engineering of vascular grafts is a growing field in regenerative medicine. Although numerous attempts have been made, the current vascular grafts made of polyurethane (PU), Dacron®, or Teflon® still display unsatisfying results. Electrospinning of biopolymers and native proteins has been in the focus of research to imitate the extracellular matrix (ECM) of vessels to produce a small caliber, off-the-shelf tissue engineered vascular graft (TEVG) as a substitute for poorly performing PU, Dacron, or Teflon prostheses. Blended poly-ε-caprolactone (PCL)/collagen grafts have shown promising results regarding biomechanical and cell supporting features. In order to find a suitable PCL/collagen blend, we fabricated plane electrospun PCL scaffolds using various collagen type I concentrations ranging from 5% to 75%. We analyzed biocompatibility and morphological aspects in vitro. Our results show beneficial features of collagen I integration regarding cell viability and functionality, but also adverse effects like the loss of a confluent monolayer at high concentrations of collagen. Furthermore, electrospun PCL scaffolds containing 25% collagen I seem to be ideal for engineering vascular grafts. Hindawi 2017 2017-08-28 /pmc/articles/PMC5592415/ /pubmed/28932749 http://dx.doi.org/10.1155/2017/9616939 Text en Copyright © 2017 Ulf Bertram et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bertram, Ulf
Steiner, Dominik
Poppitz, Benjamin
Dippold, Dirk
Köhn, Katrin
Beier, Justus P.
Detsch, Rainer
Boccaccini, Aldo R.
Schubert, Dirk W.
Horch, Raymund E.
Arkudas, Andreas
Vascular Tissue Engineering: Effects of Integrating Collagen into a PCL Based Nanofiber Material
title Vascular Tissue Engineering: Effects of Integrating Collagen into a PCL Based Nanofiber Material
title_full Vascular Tissue Engineering: Effects of Integrating Collagen into a PCL Based Nanofiber Material
title_fullStr Vascular Tissue Engineering: Effects of Integrating Collagen into a PCL Based Nanofiber Material
title_full_unstemmed Vascular Tissue Engineering: Effects of Integrating Collagen into a PCL Based Nanofiber Material
title_short Vascular Tissue Engineering: Effects of Integrating Collagen into a PCL Based Nanofiber Material
title_sort vascular tissue engineering: effects of integrating collagen into a pcl based nanofiber material
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5592415/
https://www.ncbi.nlm.nih.gov/pubmed/28932749
http://dx.doi.org/10.1155/2017/9616939
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