Cargando…
The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells
Lung cancer is a leading cause of cancer-related mortalities worldwide. In the present study, a comparison of To determine the roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells expression levels between normal lung tissues and lung cancer tissues were compared using...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5592856/ https://www.ncbi.nlm.nih.gov/pubmed/28943961 http://dx.doi.org/10.3892/ol.2017.6729 |
_version_ | 1783262946789425152 |
---|---|
author | Teng, Ji-Ping Yang, Zhi-Ying Zhu, Yu-Ming Ni, Da Zhu, Zhi-Jun Li, Xiao-Qiang |
author_facet | Teng, Ji-Ping Yang, Zhi-Ying Zhu, Yu-Ming Ni, Da Zhu, Zhi-Jun Li, Xiao-Qiang |
author_sort | Teng, Ji-Ping |
collection | PubMed |
description | Lung cancer is a leading cause of cancer-related mortalities worldwide. In the present study, a comparison of To determine the roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells expression levels between normal lung tissues and lung cancer tissues were compared using immunoblotting, and CCK-8 and Transwell assays. Lung cancer tissues had a decreased ARHGAP10 mRNA expression level compared to the adjacent normal tissues. The ectopic expression of ARHGAP10 significantly suppressed the migration, invasion and proliferation of lung cancer cells. Gene set enrichment analysis revealed that metastasis and Wnt signaling pathways were negatively correlated with ARHGAP10 expression. Immunoblotting analysis revealed that ARHGAP10 overexpression inhibited metastasis [matrix metalloproteinase (MMP)-2, MMP-9 and VEGF] and the expression of Wnt pathway-related proteins (β-catenin and c-Myc). Moreover, the stimulation effects of lithium chloride, a GSK3β inhibitor, on the accumulation of β-catenin were notably suppressed by ARHGAP10 overexpression. Collectively, ARHGAP10 acts to suppress tumor within lung cancer by affecting metastasis and Wnt signaling pathways. The results therefore suggest that ARHGAP10 is a potentially attractive target for the treatment of lung cancer. |
format | Online Article Text |
id | pubmed-5592856 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-55928562017-09-22 The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells Teng, Ji-Ping Yang, Zhi-Ying Zhu, Yu-Ming Ni, Da Zhu, Zhi-Jun Li, Xiao-Qiang Oncol Lett Articles Lung cancer is a leading cause of cancer-related mortalities worldwide. In the present study, a comparison of To determine the roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells expression levels between normal lung tissues and lung cancer tissues were compared using immunoblotting, and CCK-8 and Transwell assays. Lung cancer tissues had a decreased ARHGAP10 mRNA expression level compared to the adjacent normal tissues. The ectopic expression of ARHGAP10 significantly suppressed the migration, invasion and proliferation of lung cancer cells. Gene set enrichment analysis revealed that metastasis and Wnt signaling pathways were negatively correlated with ARHGAP10 expression. Immunoblotting analysis revealed that ARHGAP10 overexpression inhibited metastasis [matrix metalloproteinase (MMP)-2, MMP-9 and VEGF] and the expression of Wnt pathway-related proteins (β-catenin and c-Myc). Moreover, the stimulation effects of lithium chloride, a GSK3β inhibitor, on the accumulation of β-catenin were notably suppressed by ARHGAP10 overexpression. Collectively, ARHGAP10 acts to suppress tumor within lung cancer by affecting metastasis and Wnt signaling pathways. The results therefore suggest that ARHGAP10 is a potentially attractive target for the treatment of lung cancer. D.A. Spandidos 2017-10 2017-08-07 /pmc/articles/PMC5592856/ /pubmed/28943961 http://dx.doi.org/10.3892/ol.2017.6729 Text en Copyright: © Teng et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Teng, Ji-Ping Yang, Zhi-Ying Zhu, Yu-Ming Ni, Da Zhu, Zhi-Jun Li, Xiao-Qiang The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells |
title | The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells |
title_full | The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells |
title_fullStr | The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells |
title_full_unstemmed | The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells |
title_short | The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells |
title_sort | roles of arhgap10 in the proliferation, migration and invasion of lung cancer cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5592856/ https://www.ncbi.nlm.nih.gov/pubmed/28943961 http://dx.doi.org/10.3892/ol.2017.6729 |
work_keys_str_mv | AT tengjiping therolesofarhgap10intheproliferationmigrationandinvasionoflungcancercells AT yangzhiying therolesofarhgap10intheproliferationmigrationandinvasionoflungcancercells AT zhuyuming therolesofarhgap10intheproliferationmigrationandinvasionoflungcancercells AT nida therolesofarhgap10intheproliferationmigrationandinvasionoflungcancercells AT zhuzhijun therolesofarhgap10intheproliferationmigrationandinvasionoflungcancercells AT lixiaoqiang therolesofarhgap10intheproliferationmigrationandinvasionoflungcancercells AT tengjiping rolesofarhgap10intheproliferationmigrationandinvasionoflungcancercells AT yangzhiying rolesofarhgap10intheproliferationmigrationandinvasionoflungcancercells AT zhuyuming rolesofarhgap10intheproliferationmigrationandinvasionoflungcancercells AT nida rolesofarhgap10intheproliferationmigrationandinvasionoflungcancercells AT zhuzhijun rolesofarhgap10intheproliferationmigrationandinvasionoflungcancercells AT lixiaoqiang rolesofarhgap10intheproliferationmigrationandinvasionoflungcancercells |