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Effect of Electro-Acupuncture on Neuroplasticity of Spinal Cord-Transected Rats

BACKGROUND: This study aimed to evaluate the effects of electro-acupuncture (EA) on neuroplasticity associated with the expressions of neurotrophic factors (NTFs) and their receptors in rats subjected to spinal cord transection (SCT). MATERIAL/METHODS: A total of 144 rats were randomly divided into...

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Autores principales: Wang, Xuyang, Ju, Shiming, Chen, Shiwen, Gao, Wenwei, Ding, Jun, Wang, Gan, Cao, Heli, Tian, Hengli, Li, Xiaoli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5592974/
https://www.ncbi.nlm.nih.gov/pubmed/28865235
http://dx.doi.org/10.12659/MSM.903056
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author Wang, Xuyang
Ju, Shiming
Chen, Shiwen
Gao, Wenwei
Ding, Jun
Wang, Gan
Cao, Heli
Tian, Hengli
Li, Xiaoli
author_facet Wang, Xuyang
Ju, Shiming
Chen, Shiwen
Gao, Wenwei
Ding, Jun
Wang, Gan
Cao, Heli
Tian, Hengli
Li, Xiaoli
author_sort Wang, Xuyang
collection PubMed
description BACKGROUND: This study aimed to evaluate the effects of electro-acupuncture (EA) on neuroplasticity associated with the expressions of neurotrophic factors (NTFs) and their receptors in rats subjected to spinal cord transection (SCT). MATERIAL/METHODS: A total of 144 rats were randomly divided into 3 groups (n=48 per group): sham-operated group, SCT group, and EA (electro-acupuncture) group. Rats in SCT and EA groups received spinal cord transection at T(10)–T(11) vertebral levels. Then, EA group rats received EA treatment. Reverse transcription polymerase chain reaction was used to detect NTFs and receptors at the mRNA level. In situ hybridization (ISH) and immunohistochemistry (IHC) were used to detect the expression of NTFs and their receptors. Basso, Beattie, Bresnahan (BBB) scores and cortical somato-sensory evoked potentials (CSEP) were evaluated to assess the recovery of motor and sensory functions. We also measured BDA (Biotinylated dextran amine) axonal tracing, CGRP (Calcitonin gene-related peptide), GAP-43 (Growth-associated protein), and synaptophysin immunohistochemistry (IHC). RESULTS: EA treatment led to obvious improvement in hindlimb locomotor and sensory functions. CNTF, FGF-2, and TrkB mRNA were significantly upregulated, while NGF, PDGF, TGF-β1, IGF-1, TrkA, and TrkC mRNA were concomitantly downregulated in the caudal spinal segment (CSS) following EA. Immunohistochemistry demonstrated an increased number of CGRP fibers, GAP-43, and synaptophysin profiles in the CSS in the EA rats. CONCLUSIONS: EA may promote the recovery of neuroplasticity in rats subjected to SCT. This could be attributed to the systematic regulation of NTFs and their receptors after EA.
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spelling pubmed-55929742017-09-15 Effect of Electro-Acupuncture on Neuroplasticity of Spinal Cord-Transected Rats Wang, Xuyang Ju, Shiming Chen, Shiwen Gao, Wenwei Ding, Jun Wang, Gan Cao, Heli Tian, Hengli Li, Xiaoli Med Sci Monit Animal Study BACKGROUND: This study aimed to evaluate the effects of electro-acupuncture (EA) on neuroplasticity associated with the expressions of neurotrophic factors (NTFs) and their receptors in rats subjected to spinal cord transection (SCT). MATERIAL/METHODS: A total of 144 rats were randomly divided into 3 groups (n=48 per group): sham-operated group, SCT group, and EA (electro-acupuncture) group. Rats in SCT and EA groups received spinal cord transection at T(10)–T(11) vertebral levels. Then, EA group rats received EA treatment. Reverse transcription polymerase chain reaction was used to detect NTFs and receptors at the mRNA level. In situ hybridization (ISH) and immunohistochemistry (IHC) were used to detect the expression of NTFs and their receptors. Basso, Beattie, Bresnahan (BBB) scores and cortical somato-sensory evoked potentials (CSEP) were evaluated to assess the recovery of motor and sensory functions. We also measured BDA (Biotinylated dextran amine) axonal tracing, CGRP (Calcitonin gene-related peptide), GAP-43 (Growth-associated protein), and synaptophysin immunohistochemistry (IHC). RESULTS: EA treatment led to obvious improvement in hindlimb locomotor and sensory functions. CNTF, FGF-2, and TrkB mRNA were significantly upregulated, while NGF, PDGF, TGF-β1, IGF-1, TrkA, and TrkC mRNA were concomitantly downregulated in the caudal spinal segment (CSS) following EA. Immunohistochemistry demonstrated an increased number of CGRP fibers, GAP-43, and synaptophysin profiles in the CSS in the EA rats. CONCLUSIONS: EA may promote the recovery of neuroplasticity in rats subjected to SCT. This could be attributed to the systematic regulation of NTFs and their receptors after EA. International Scientific Literature, Inc. 2017-09-02 /pmc/articles/PMC5592974/ /pubmed/28865235 http://dx.doi.org/10.12659/MSM.903056 Text en © Med Sci Monit, 2017 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Animal Study
Wang, Xuyang
Ju, Shiming
Chen, Shiwen
Gao, Wenwei
Ding, Jun
Wang, Gan
Cao, Heli
Tian, Hengli
Li, Xiaoli
Effect of Electro-Acupuncture on Neuroplasticity of Spinal Cord-Transected Rats
title Effect of Electro-Acupuncture on Neuroplasticity of Spinal Cord-Transected Rats
title_full Effect of Electro-Acupuncture on Neuroplasticity of Spinal Cord-Transected Rats
title_fullStr Effect of Electro-Acupuncture on Neuroplasticity of Spinal Cord-Transected Rats
title_full_unstemmed Effect of Electro-Acupuncture on Neuroplasticity of Spinal Cord-Transected Rats
title_short Effect of Electro-Acupuncture on Neuroplasticity of Spinal Cord-Transected Rats
title_sort effect of electro-acupuncture on neuroplasticity of spinal cord-transected rats
topic Animal Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5592974/
https://www.ncbi.nlm.nih.gov/pubmed/28865235
http://dx.doi.org/10.12659/MSM.903056
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