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Effect of Electro-Acupuncture on Neuroplasticity of Spinal Cord-Transected Rats
BACKGROUND: This study aimed to evaluate the effects of electro-acupuncture (EA) on neuroplasticity associated with the expressions of neurotrophic factors (NTFs) and their receptors in rats subjected to spinal cord transection (SCT). MATERIAL/METHODS: A total of 144 rats were randomly divided into...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5592974/ https://www.ncbi.nlm.nih.gov/pubmed/28865235 http://dx.doi.org/10.12659/MSM.903056 |
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author | Wang, Xuyang Ju, Shiming Chen, Shiwen Gao, Wenwei Ding, Jun Wang, Gan Cao, Heli Tian, Hengli Li, Xiaoli |
author_facet | Wang, Xuyang Ju, Shiming Chen, Shiwen Gao, Wenwei Ding, Jun Wang, Gan Cao, Heli Tian, Hengli Li, Xiaoli |
author_sort | Wang, Xuyang |
collection | PubMed |
description | BACKGROUND: This study aimed to evaluate the effects of electro-acupuncture (EA) on neuroplasticity associated with the expressions of neurotrophic factors (NTFs) and their receptors in rats subjected to spinal cord transection (SCT). MATERIAL/METHODS: A total of 144 rats were randomly divided into 3 groups (n=48 per group): sham-operated group, SCT group, and EA (electro-acupuncture) group. Rats in SCT and EA groups received spinal cord transection at T(10)–T(11) vertebral levels. Then, EA group rats received EA treatment. Reverse transcription polymerase chain reaction was used to detect NTFs and receptors at the mRNA level. In situ hybridization (ISH) and immunohistochemistry (IHC) were used to detect the expression of NTFs and their receptors. Basso, Beattie, Bresnahan (BBB) scores and cortical somato-sensory evoked potentials (CSEP) were evaluated to assess the recovery of motor and sensory functions. We also measured BDA (Biotinylated dextran amine) axonal tracing, CGRP (Calcitonin gene-related peptide), GAP-43 (Growth-associated protein), and synaptophysin immunohistochemistry (IHC). RESULTS: EA treatment led to obvious improvement in hindlimb locomotor and sensory functions. CNTF, FGF-2, and TrkB mRNA were significantly upregulated, while NGF, PDGF, TGF-β1, IGF-1, TrkA, and TrkC mRNA were concomitantly downregulated in the caudal spinal segment (CSS) following EA. Immunohistochemistry demonstrated an increased number of CGRP fibers, GAP-43, and synaptophysin profiles in the CSS in the EA rats. CONCLUSIONS: EA may promote the recovery of neuroplasticity in rats subjected to SCT. This could be attributed to the systematic regulation of NTFs and their receptors after EA. |
format | Online Article Text |
id | pubmed-5592974 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-55929742017-09-15 Effect of Electro-Acupuncture on Neuroplasticity of Spinal Cord-Transected Rats Wang, Xuyang Ju, Shiming Chen, Shiwen Gao, Wenwei Ding, Jun Wang, Gan Cao, Heli Tian, Hengli Li, Xiaoli Med Sci Monit Animal Study BACKGROUND: This study aimed to evaluate the effects of electro-acupuncture (EA) on neuroplasticity associated with the expressions of neurotrophic factors (NTFs) and their receptors in rats subjected to spinal cord transection (SCT). MATERIAL/METHODS: A total of 144 rats were randomly divided into 3 groups (n=48 per group): sham-operated group, SCT group, and EA (electro-acupuncture) group. Rats in SCT and EA groups received spinal cord transection at T(10)–T(11) vertebral levels. Then, EA group rats received EA treatment. Reverse transcription polymerase chain reaction was used to detect NTFs and receptors at the mRNA level. In situ hybridization (ISH) and immunohistochemistry (IHC) were used to detect the expression of NTFs and their receptors. Basso, Beattie, Bresnahan (BBB) scores and cortical somato-sensory evoked potentials (CSEP) were evaluated to assess the recovery of motor and sensory functions. We also measured BDA (Biotinylated dextran amine) axonal tracing, CGRP (Calcitonin gene-related peptide), GAP-43 (Growth-associated protein), and synaptophysin immunohistochemistry (IHC). RESULTS: EA treatment led to obvious improvement in hindlimb locomotor and sensory functions. CNTF, FGF-2, and TrkB mRNA were significantly upregulated, while NGF, PDGF, TGF-β1, IGF-1, TrkA, and TrkC mRNA were concomitantly downregulated in the caudal spinal segment (CSS) following EA. Immunohistochemistry demonstrated an increased number of CGRP fibers, GAP-43, and synaptophysin profiles in the CSS in the EA rats. CONCLUSIONS: EA may promote the recovery of neuroplasticity in rats subjected to SCT. This could be attributed to the systematic regulation of NTFs and their receptors after EA. International Scientific Literature, Inc. 2017-09-02 /pmc/articles/PMC5592974/ /pubmed/28865235 http://dx.doi.org/10.12659/MSM.903056 Text en © Med Sci Monit, 2017 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) ) |
spellingShingle | Animal Study Wang, Xuyang Ju, Shiming Chen, Shiwen Gao, Wenwei Ding, Jun Wang, Gan Cao, Heli Tian, Hengli Li, Xiaoli Effect of Electro-Acupuncture on Neuroplasticity of Spinal Cord-Transected Rats |
title | Effect of Electro-Acupuncture on Neuroplasticity of Spinal Cord-Transected Rats |
title_full | Effect of Electro-Acupuncture on Neuroplasticity of Spinal Cord-Transected Rats |
title_fullStr | Effect of Electro-Acupuncture on Neuroplasticity of Spinal Cord-Transected Rats |
title_full_unstemmed | Effect of Electro-Acupuncture on Neuroplasticity of Spinal Cord-Transected Rats |
title_short | Effect of Electro-Acupuncture on Neuroplasticity of Spinal Cord-Transected Rats |
title_sort | effect of electro-acupuncture on neuroplasticity of spinal cord-transected rats |
topic | Animal Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5592974/ https://www.ncbi.nlm.nih.gov/pubmed/28865235 http://dx.doi.org/10.12659/MSM.903056 |
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