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Endothelial cell-derived exosomes protect SH-SY5Y nerve cells against ischemia/reperfusion injury

Cerebral ischemia is a leading cause of death and disability. A previous study indicated that remote ischemic postconditioning (RIP) in the treatment of cerebral ischemia reduces ischemia/reperfusion (I/R) injury. However, the underlying mechanism is not well understood. In the present study, the au...

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Autores principales: Xiao, Bing, Chai, Yi, Lv, Shigang, Ye, Minhua, Wu, Miaojing, Xie, Liyuan, Fan, Yanghua, Zhu, Xingen, Gao, Ziyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5593464/
https://www.ncbi.nlm.nih.gov/pubmed/28849073
http://dx.doi.org/10.3892/ijmm.2017.3106
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author Xiao, Bing
Chai, Yi
Lv, Shigang
Ye, Minhua
Wu, Miaojing
Xie, Liyuan
Fan, Yanghua
Zhu, Xingen
Gao, Ziyun
author_facet Xiao, Bing
Chai, Yi
Lv, Shigang
Ye, Minhua
Wu, Miaojing
Xie, Liyuan
Fan, Yanghua
Zhu, Xingen
Gao, Ziyun
author_sort Xiao, Bing
collection PubMed
description Cerebral ischemia is a leading cause of death and disability. A previous study indicated that remote ischemic postconditioning (RIP) in the treatment of cerebral ischemia reduces ischemia/reperfusion (I/R) injury. However, the underlying mechanism is not well understood. In the present study, the authors hypothesized that the protective effect of RIP on neurological damage is mediated by exosomes that are released by endothelial cells in femoral arteries. To test this, right middle cerebral artery occlusion/reperfusion with RIP was performed in rats. In addition, an I/R injury cell model was tested that included human umbilical vein endothelial cells (HUVECs) and SH-SY5Y cells. Both the in vivo and in vitro models were examined for injury. Markers of exosomes (CD63, HSP70 and TSG101) were assessed by immunohistochemistry, western blot analysis and flow cytometry. Exosomes were extracted from both animal serum and HUVEC culture medium and identified by electron microscopy. They investigated the role of endothelial cell-derived exosomes in the proliferation, apoptosis, cell cycle, migration and invasion of I/R-injured SH-SY5Y cells. In addition, apoptosis-related molecules caspase-3, Bax and Bcl-2 were detected. RIP was determined to increase the number of exosomes and the expression levels of CD63, HSP70 and TSG101 in plasma, but not in brain hippocampal tissue. The size of exosomes released after I/R in HUVECs was similar to the size of exosomes released in rats subjected to RIP. Endothelial cell-derived exosomes partly suppressed the I/R-induced cell cycle arrest and apoptosis, and inhibited cell proliferation, migration and invasion in SH-SY5Y nerve cells. Endothelial cell-derived exosomes directly protect nerve cells against I/R injury, and are responsible for the protective role of RIP in I/R.
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spelling pubmed-55934642017-09-22 Endothelial cell-derived exosomes protect SH-SY5Y nerve cells against ischemia/reperfusion injury Xiao, Bing Chai, Yi Lv, Shigang Ye, Minhua Wu, Miaojing Xie, Liyuan Fan, Yanghua Zhu, Xingen Gao, Ziyun Int J Mol Med Articles Cerebral ischemia is a leading cause of death and disability. A previous study indicated that remote ischemic postconditioning (RIP) in the treatment of cerebral ischemia reduces ischemia/reperfusion (I/R) injury. However, the underlying mechanism is not well understood. In the present study, the authors hypothesized that the protective effect of RIP on neurological damage is mediated by exosomes that are released by endothelial cells in femoral arteries. To test this, right middle cerebral artery occlusion/reperfusion with RIP was performed in rats. In addition, an I/R injury cell model was tested that included human umbilical vein endothelial cells (HUVECs) and SH-SY5Y cells. Both the in vivo and in vitro models were examined for injury. Markers of exosomes (CD63, HSP70 and TSG101) were assessed by immunohistochemistry, western blot analysis and flow cytometry. Exosomes were extracted from both animal serum and HUVEC culture medium and identified by electron microscopy. They investigated the role of endothelial cell-derived exosomes in the proliferation, apoptosis, cell cycle, migration and invasion of I/R-injured SH-SY5Y cells. In addition, apoptosis-related molecules caspase-3, Bax and Bcl-2 were detected. RIP was determined to increase the number of exosomes and the expression levels of CD63, HSP70 and TSG101 in plasma, but not in brain hippocampal tissue. The size of exosomes released after I/R in HUVECs was similar to the size of exosomes released in rats subjected to RIP. Endothelial cell-derived exosomes partly suppressed the I/R-induced cell cycle arrest and apoptosis, and inhibited cell proliferation, migration and invasion in SH-SY5Y nerve cells. Endothelial cell-derived exosomes directly protect nerve cells against I/R injury, and are responsible for the protective role of RIP in I/R. D.A. Spandidos 2017-10 2017-08-23 /pmc/articles/PMC5593464/ /pubmed/28849073 http://dx.doi.org/10.3892/ijmm.2017.3106 Text en Copyright: © Xiao et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Xiao, Bing
Chai, Yi
Lv, Shigang
Ye, Minhua
Wu, Miaojing
Xie, Liyuan
Fan, Yanghua
Zhu, Xingen
Gao, Ziyun
Endothelial cell-derived exosomes protect SH-SY5Y nerve cells against ischemia/reperfusion injury
title Endothelial cell-derived exosomes protect SH-SY5Y nerve cells against ischemia/reperfusion injury
title_full Endothelial cell-derived exosomes protect SH-SY5Y nerve cells against ischemia/reperfusion injury
title_fullStr Endothelial cell-derived exosomes protect SH-SY5Y nerve cells against ischemia/reperfusion injury
title_full_unstemmed Endothelial cell-derived exosomes protect SH-SY5Y nerve cells against ischemia/reperfusion injury
title_short Endothelial cell-derived exosomes protect SH-SY5Y nerve cells against ischemia/reperfusion injury
title_sort endothelial cell-derived exosomes protect sh-sy5y nerve cells against ischemia/reperfusion injury
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5593464/
https://www.ncbi.nlm.nih.gov/pubmed/28849073
http://dx.doi.org/10.3892/ijmm.2017.3106
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