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Dysregulation of an X-linked primate-specific epididymal microRNA cluster in unexplained asthenozoospermia
Asthenoszoopermia, characterized by reduced sperm motility, is one of the primary forms of male infertility. Whereas most cases were diagnosed into unexplained asthenozoospermia (UA) because the etiology cannot be identified. In animal models, epigenetic dysregulation in epididymis can impair sperm...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5593606/ https://www.ncbi.nlm.nih.gov/pubmed/28915635 http://dx.doi.org/10.18632/oncotarget.18076 |
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author | Qing, Xingrong Shi, Jian Dong, Tingting Wu, Chunlin Hu, Lian Li, Honggang |
author_facet | Qing, Xingrong Shi, Jian Dong, Tingting Wu, Chunlin Hu, Lian Li, Honggang |
author_sort | Qing, Xingrong |
collection | PubMed |
description | Asthenoszoopermia, characterized by reduced sperm motility, is one of the primary forms of male infertility. Whereas most cases were diagnosed into unexplained asthenozoospermia (UA) because the etiology cannot be identified. In animal models, epigenetic dysregulation in epididymis can impair sperm maturation and result in asthenozoospermia. However, researches of epididymal epigenetic regulation on humans are impeded by the difficulty in obtaining epididymal tissues. We previously identified cell-free seminal microRNAs predominately derived from epididymis in human ejaculate. In the present study, these microRNAs were used to screen and validate the microRNA dysregulation in men with UA, which were divided into screening set and validation set. The expression of five miRNAs (miR-891b, miR-892b, miR-892a, miR-888 and miR-890) was found and confirmed to be dysregulated in men with UA. Interestingly, these five miRNAs belong to a primate-specific miRNA cluster located on the X chromosome with epididymis specific expression. Moreover, obvious coherent dysregulation of these miRNAs were observed in 13% men with UA. Regression analysis demonstrated that levels of these miRNAs were significantly correlated with progressive sperm motility. Functions and pathways of predicted target genes of this cluster suggested its role in sperm maturation. Dysregulation of this miRNA cluster might be an epigenetic basis for some patients with UA. We also showed a noninvasive and feasible approach to get epigenetic information of human epididymis. |
format | Online Article Text |
id | pubmed-5593606 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-55936062017-09-14 Dysregulation of an X-linked primate-specific epididymal microRNA cluster in unexplained asthenozoospermia Qing, Xingrong Shi, Jian Dong, Tingting Wu, Chunlin Hu, Lian Li, Honggang Oncotarget Research Paper Asthenoszoopermia, characterized by reduced sperm motility, is one of the primary forms of male infertility. Whereas most cases were diagnosed into unexplained asthenozoospermia (UA) because the etiology cannot be identified. In animal models, epigenetic dysregulation in epididymis can impair sperm maturation and result in asthenozoospermia. However, researches of epididymal epigenetic regulation on humans are impeded by the difficulty in obtaining epididymal tissues. We previously identified cell-free seminal microRNAs predominately derived from epididymis in human ejaculate. In the present study, these microRNAs were used to screen and validate the microRNA dysregulation in men with UA, which were divided into screening set and validation set. The expression of five miRNAs (miR-891b, miR-892b, miR-892a, miR-888 and miR-890) was found and confirmed to be dysregulated in men with UA. Interestingly, these five miRNAs belong to a primate-specific miRNA cluster located on the X chromosome with epididymis specific expression. Moreover, obvious coherent dysregulation of these miRNAs were observed in 13% men with UA. Regression analysis demonstrated that levels of these miRNAs were significantly correlated with progressive sperm motility. Functions and pathways of predicted target genes of this cluster suggested its role in sperm maturation. Dysregulation of this miRNA cluster might be an epigenetic basis for some patients with UA. We also showed a noninvasive and feasible approach to get epigenetic information of human epididymis. Impact Journals LLC 2017-05-23 /pmc/articles/PMC5593606/ /pubmed/28915635 http://dx.doi.org/10.18632/oncotarget.18076 Text en Copyright: © 2017 Qing et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Research Paper Qing, Xingrong Shi, Jian Dong, Tingting Wu, Chunlin Hu, Lian Li, Honggang Dysregulation of an X-linked primate-specific epididymal microRNA cluster in unexplained asthenozoospermia |
title | Dysregulation of an X-linked primate-specific epididymal microRNA cluster in unexplained asthenozoospermia |
title_full | Dysregulation of an X-linked primate-specific epididymal microRNA cluster in unexplained asthenozoospermia |
title_fullStr | Dysregulation of an X-linked primate-specific epididymal microRNA cluster in unexplained asthenozoospermia |
title_full_unstemmed | Dysregulation of an X-linked primate-specific epididymal microRNA cluster in unexplained asthenozoospermia |
title_short | Dysregulation of an X-linked primate-specific epididymal microRNA cluster in unexplained asthenozoospermia |
title_sort | dysregulation of an x-linked primate-specific epididymal microrna cluster in unexplained asthenozoospermia |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5593606/ https://www.ncbi.nlm.nih.gov/pubmed/28915635 http://dx.doi.org/10.18632/oncotarget.18076 |
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