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Oncolytic peptide LTX-315 induces an immune-mediated abscopal effect in a rat sarcoma model

LTX 315 is an oncolytic peptide with potent immunological properties. In the present study, we demonstrate that intratumoral treatment with LTX-315 resulted in a complete regression and systemic immune response in a rat fibrosarcoma model. The treatment was T-cell dependent, and also resulted in an...

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Autores principales: Nestvold, Janne, Wang, Meng-Yu, Camilio, Ketil A., Zinöcker, Severin, Tjelle, Torunn Elisabeth, Lindberg, Alf, Haug, Bengt Erik, Kvalheim, Gunnar, Sveinbjørnsson, Baldur, Rekdal, Øystein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5593701/
https://www.ncbi.nlm.nih.gov/pubmed/28920000
http://dx.doi.org/10.1080/2162402X.2017.1338236
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author Nestvold, Janne
Wang, Meng-Yu
Camilio, Ketil A.
Zinöcker, Severin
Tjelle, Torunn Elisabeth
Lindberg, Alf
Haug, Bengt Erik
Kvalheim, Gunnar
Sveinbjørnsson, Baldur
Rekdal, Øystein
author_facet Nestvold, Janne
Wang, Meng-Yu
Camilio, Ketil A.
Zinöcker, Severin
Tjelle, Torunn Elisabeth
Lindberg, Alf
Haug, Bengt Erik
Kvalheim, Gunnar
Sveinbjørnsson, Baldur
Rekdal, Øystein
author_sort Nestvold, Janne
collection PubMed
description LTX 315 is an oncolytic peptide with potent immunological properties. In the present study, we demonstrate that intratumoral treatment with LTX-315 resulted in a complete regression and systemic immune response in a rat fibrosarcoma model. The treatment was T-cell dependent, and also resulted in an abscopal effect as demonstrated by the regression of distal non-treated lesions. Significant infiltration of CD8(+) T cells was observed in both treated and non-treated lesions, as shown by immunohistochemical and flow cytometric analysis. LTX-315 rapidly killed the cells in vitro with a lytic mode of action followed by the subsequent release of Danger-Associated Molecular Pattern (DAMP) molecules such as HMGB1, ATP and Cytochrome c. Together, our data demonstrate that LTX-315 represents a new approach to cancer immunotherapy, which has the potential as a novel immunotherapeutic agent.
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spelling pubmed-55937012017-09-15 Oncolytic peptide LTX-315 induces an immune-mediated abscopal effect in a rat sarcoma model Nestvold, Janne Wang, Meng-Yu Camilio, Ketil A. Zinöcker, Severin Tjelle, Torunn Elisabeth Lindberg, Alf Haug, Bengt Erik Kvalheim, Gunnar Sveinbjørnsson, Baldur Rekdal, Øystein Oncoimmunology Original Research LTX 315 is an oncolytic peptide with potent immunological properties. In the present study, we demonstrate that intratumoral treatment with LTX-315 resulted in a complete regression and systemic immune response in a rat fibrosarcoma model. The treatment was T-cell dependent, and also resulted in an abscopal effect as demonstrated by the regression of distal non-treated lesions. Significant infiltration of CD8(+) T cells was observed in both treated and non-treated lesions, as shown by immunohistochemical and flow cytometric analysis. LTX-315 rapidly killed the cells in vitro with a lytic mode of action followed by the subsequent release of Danger-Associated Molecular Pattern (DAMP) molecules such as HMGB1, ATP and Cytochrome c. Together, our data demonstrate that LTX-315 represents a new approach to cancer immunotherapy, which has the potential as a novel immunotherapeutic agent. Taylor & Francis 2017-06-16 /pmc/articles/PMC5593701/ /pubmed/28920000 http://dx.doi.org/10.1080/2162402X.2017.1338236 Text en © 2017 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
spellingShingle Original Research
Nestvold, Janne
Wang, Meng-Yu
Camilio, Ketil A.
Zinöcker, Severin
Tjelle, Torunn Elisabeth
Lindberg, Alf
Haug, Bengt Erik
Kvalheim, Gunnar
Sveinbjørnsson, Baldur
Rekdal, Øystein
Oncolytic peptide LTX-315 induces an immune-mediated abscopal effect in a rat sarcoma model
title Oncolytic peptide LTX-315 induces an immune-mediated abscopal effect in a rat sarcoma model
title_full Oncolytic peptide LTX-315 induces an immune-mediated abscopal effect in a rat sarcoma model
title_fullStr Oncolytic peptide LTX-315 induces an immune-mediated abscopal effect in a rat sarcoma model
title_full_unstemmed Oncolytic peptide LTX-315 induces an immune-mediated abscopal effect in a rat sarcoma model
title_short Oncolytic peptide LTX-315 induces an immune-mediated abscopal effect in a rat sarcoma model
title_sort oncolytic peptide ltx-315 induces an immune-mediated abscopal effect in a rat sarcoma model
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5593701/
https://www.ncbi.nlm.nih.gov/pubmed/28920000
http://dx.doi.org/10.1080/2162402X.2017.1338236
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